Limits...
Identification of a bipotential precursor cell in hepatic cell lines derived from transgenic mice expressing cyto-Met in the liver.

Spagnoli FM, Amicone L, Tripodi M, Weiss MC - J. Cell Biol. (1998)

Bottom Line: Palmate cells show none of these properties.Derivation of epithelial from palmate cells is confirmed by characterization of the progeny of individually fished cells.The clonal isolation of the palmate cell, an immortalized nontransformed bipotential cell that does not yet express the liver-enriched transcription factors and is a precursor of the epithelial-hepatocyte in MMH lines, provides a new tool for the study of mechanisms controlling liver development.

View Article: PubMed Central - PubMed

Affiliation: Unité de Génétique de la Différenciation, URA 1773 du Centre National de la Recherche Scientifique, Institut Pasteur, 75724 Paris Cedex 15, France.

ABSTRACT
Met murine hepatocyte (MMH) lines were established from livers of transgenic mice expressing constitutively active human Met. These lines harbor two cell types: epithelial cells resembling the parental populations and flattened cells with multiple projections and a dispersed growth habit that are designated palmate. Epithelial cells express the liver-enriched transcription factors HNF4 and HNF1alpha, and proteins associated with epithelial cell differentiation. Treatments that modulate their differentiation state, including acidic FGF, induce hepatic functions. Palmate cells show none of these properties. However, they can differentiate along the hepatic cell lineage, giving rise to: (a) epithelial cells that express hepatic transcription factors and are competent to express hepatic functions; (b) bile duct-like structures in three-dimensional Matrigel cultures. Derivation of epithelial from palmate cells is confirmed by characterization of the progeny of individually fished cells. Furthermore, karyotype analysis confirms the direction of the phenotypic transition: palmate cells are diploid and the epithelial cells are hypotetraploid. The clonal isolation of the palmate cell, an immortalized nontransformed bipotential cell that does not yet express the liver-enriched transcription factors and is a precursor of the epithelial-hepatocyte in MMH lines, provides a new tool for the study of mechanisms controlling liver development.

Show MeSH
Morphology of clones derived from the MMH E14 line.  Phase-contrast micrographs of the epithelial and the palmate  clones at low (top) and high or intermediate (bottom) cell density,  respectively. In the micrographs of the cultures at low density the  differences in growth habit and cellular shape between the epithelial and the palmate clone are highlighted; those at higher density document that these differences are maintained. Bar, 40 μm.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2132947&req=5

Figure 1: Morphology of clones derived from the MMH E14 line. Phase-contrast micrographs of the epithelial and the palmate clones at low (top) and high or intermediate (bottom) cell density, respectively. In the micrographs of the cultures at low density the differences in growth habit and cellular shape between the epithelial and the palmate clone are highlighted; those at higher density document that these differences are maintained. Bar, 40 μm.

Mentions: Fig. 1 shows the morphological characteristics of the two classes of clones. Although cells of both types of clones exhibit generation times of ∼24 h, striking differences concern: (a) the cell shape and (b) the growth habit. One class (referred to as epithelial) shows flattened epithelial morphology, similar to the parental lines (Amicone et al., 1997): the cells have a regular polygonal shape, with clear round nuclei, and grow in a tightly packed fashion. Cells of the second class show a flat fibroblast-like morphology: they are strongly attached to the dish, grow in a scattered and loosely packed fashion, and send out cytoplasmic extensions. To remain openminded concerning the origin of cells of this second type and to recall their characteristic form, we shall refer to them as palmate rather than fibroblast-like.


Identification of a bipotential precursor cell in hepatic cell lines derived from transgenic mice expressing cyto-Met in the liver.

Spagnoli FM, Amicone L, Tripodi M, Weiss MC - J. Cell Biol. (1998)

Morphology of clones derived from the MMH E14 line.  Phase-contrast micrographs of the epithelial and the palmate  clones at low (top) and high or intermediate (bottom) cell density,  respectively. In the micrographs of the cultures at low density the  differences in growth habit and cellular shape between the epithelial and the palmate clone are highlighted; those at higher density document that these differences are maintained. Bar, 40 μm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2132947&req=5

Figure 1: Morphology of clones derived from the MMH E14 line. Phase-contrast micrographs of the epithelial and the palmate clones at low (top) and high or intermediate (bottom) cell density, respectively. In the micrographs of the cultures at low density the differences in growth habit and cellular shape between the epithelial and the palmate clone are highlighted; those at higher density document that these differences are maintained. Bar, 40 μm.
Mentions: Fig. 1 shows the morphological characteristics of the two classes of clones. Although cells of both types of clones exhibit generation times of ∼24 h, striking differences concern: (a) the cell shape and (b) the growth habit. One class (referred to as epithelial) shows flattened epithelial morphology, similar to the parental lines (Amicone et al., 1997): the cells have a regular polygonal shape, with clear round nuclei, and grow in a tightly packed fashion. Cells of the second class show a flat fibroblast-like morphology: they are strongly attached to the dish, grow in a scattered and loosely packed fashion, and send out cytoplasmic extensions. To remain openminded concerning the origin of cells of this second type and to recall their characteristic form, we shall refer to them as palmate rather than fibroblast-like.

Bottom Line: Palmate cells show none of these properties.Derivation of epithelial from palmate cells is confirmed by characterization of the progeny of individually fished cells.The clonal isolation of the palmate cell, an immortalized nontransformed bipotential cell that does not yet express the liver-enriched transcription factors and is a precursor of the epithelial-hepatocyte in MMH lines, provides a new tool for the study of mechanisms controlling liver development.

View Article: PubMed Central - PubMed

Affiliation: Unité de Génétique de la Différenciation, URA 1773 du Centre National de la Recherche Scientifique, Institut Pasteur, 75724 Paris Cedex 15, France.

ABSTRACT
Met murine hepatocyte (MMH) lines were established from livers of transgenic mice expressing constitutively active human Met. These lines harbor two cell types: epithelial cells resembling the parental populations and flattened cells with multiple projections and a dispersed growth habit that are designated palmate. Epithelial cells express the liver-enriched transcription factors HNF4 and HNF1alpha, and proteins associated with epithelial cell differentiation. Treatments that modulate their differentiation state, including acidic FGF, induce hepatic functions. Palmate cells show none of these properties. However, they can differentiate along the hepatic cell lineage, giving rise to: (a) epithelial cells that express hepatic transcription factors and are competent to express hepatic functions; (b) bile duct-like structures in three-dimensional Matrigel cultures. Derivation of epithelial from palmate cells is confirmed by characterization of the progeny of individually fished cells. Furthermore, karyotype analysis confirms the direction of the phenotypic transition: palmate cells are diploid and the epithelial cells are hypotetraploid. The clonal isolation of the palmate cell, an immortalized nontransformed bipotential cell that does not yet express the liver-enriched transcription factors and is a precursor of the epithelial-hepatocyte in MMH lines, provides a new tool for the study of mechanisms controlling liver development.

Show MeSH