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LYVE-1, a new homologue of the CD44 glycoprotein, is a lymph-specific receptor for hyaluronan.

Banerji S, Ni J, Wang SX, Clasper S, Su J, Tammi R, Jones M, Jackson DG - J. Cell Biol. (1999)

Bottom Line: Like CD44, the LYVE-1 molecule binds both soluble and immobilized HA.However, unlike CD44, the LYVE-1 molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels.Hence, LYVE-1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves.

View Article: PubMed Central - PubMed

Affiliation: University of Oxford, Molecular Immunology Group, Nuffield Department of Medicine, John Radcliff Hospital, Headington, Oxford OX3 9DU, United Kingdom.

ABSTRACT
The extracellular matrix glycosaminoglycan hyaluronan (HA) is an abundant component of skin and mesenchymal tissues where it facilitates cell migration during wound healing, inflammation, and embryonic morphogenesis. Both during normal tissue homeostasis and particularly after tissue injury, HA is mobilized from these sites through lymphatic vessels to the lymph nodes where it is degraded before entering the circulation for rapid uptake by the liver. Currently, however, the identities of HA binding molecules which control this pathway are unknown. Here we describe the first such molecule, LYVE-1, which we have identified as a major receptor for HA on the lymph vessel wall. The deduced amino acid sequence of LYVE-1 predicts a 322-residue type I integral membrane polypeptide 41% similar to the CD44 HA receptor with a 212-residue extracellular domain containing a single Link module the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE-1 molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE-1 molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE-1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves.

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Related in: MedlinePlus

RT-PCR analysis of LYVE-1 mRNA  expression in tissue culture cell lines. LYVE-1  and CD44 mRNA levels were compared within a  panel of tissue culture cell lines representing different lineages. Samples of cDNA prepared  from each of the cell lines shown were amplified  with primers specific for either LYVE-1 (top),  CD44 (middle), or glyceraldehyde-3-phosphate  dehydrogenase (control, bottom) followed by  electrophoresis on 1.25% agarose gels, staining  with ethidium bromide (glyceraldehyde-3-phosphate dehydrogenase only), or Southern blotting  and hybridization with the appropriate 32P-labeled detection oligonucleotides as described  in Materials and Methods. Arrows depict the  sizes of the PCR products in each case.
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Figure 6: RT-PCR analysis of LYVE-1 mRNA expression in tissue culture cell lines. LYVE-1 and CD44 mRNA levels were compared within a panel of tissue culture cell lines representing different lineages. Samples of cDNA prepared from each of the cell lines shown were amplified with primers specific for either LYVE-1 (top), CD44 (middle), or glyceraldehyde-3-phosphate dehydrogenase (control, bottom) followed by electrophoresis on 1.25% agarose gels, staining with ethidium bromide (glyceraldehyde-3-phosphate dehydrogenase only), or Southern blotting and hybridization with the appropriate 32P-labeled detection oligonucleotides as described in Materials and Methods. Arrows depict the sizes of the PCR products in each case.

Mentions: Intriguingly, RT-PCR analyses (Fig. 6) of individual cell lines derived from lymphocytes, myeloid cells, monocytes, microvascular endothelial cells, and fibroblasts yielded no products in any cell type with the exception of late passage HUVEC, and placental tissue, used as a positive control. In contrast, each cell line yielded abundant PCR products when amplified with primers to CD44, or the housekeeping gene glyceraldehyde-3-phosphate dehydrogenase. These results suggested an unusually specific pattern of tissue expression for LYVE-1 that was clearly distinct from that of the CD44 molecule.


LYVE-1, a new homologue of the CD44 glycoprotein, is a lymph-specific receptor for hyaluronan.

Banerji S, Ni J, Wang SX, Clasper S, Su J, Tammi R, Jones M, Jackson DG - J. Cell Biol. (1999)

RT-PCR analysis of LYVE-1 mRNA  expression in tissue culture cell lines. LYVE-1  and CD44 mRNA levels were compared within a  panel of tissue culture cell lines representing different lineages. Samples of cDNA prepared  from each of the cell lines shown were amplified  with primers specific for either LYVE-1 (top),  CD44 (middle), or glyceraldehyde-3-phosphate  dehydrogenase (control, bottom) followed by  electrophoresis on 1.25% agarose gels, staining  with ethidium bromide (glyceraldehyde-3-phosphate dehydrogenase only), or Southern blotting  and hybridization with the appropriate 32P-labeled detection oligonucleotides as described  in Materials and Methods. Arrows depict the  sizes of the PCR products in each case.
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Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2132933&req=5

Figure 6: RT-PCR analysis of LYVE-1 mRNA expression in tissue culture cell lines. LYVE-1 and CD44 mRNA levels were compared within a panel of tissue culture cell lines representing different lineages. Samples of cDNA prepared from each of the cell lines shown were amplified with primers specific for either LYVE-1 (top), CD44 (middle), or glyceraldehyde-3-phosphate dehydrogenase (control, bottom) followed by electrophoresis on 1.25% agarose gels, staining with ethidium bromide (glyceraldehyde-3-phosphate dehydrogenase only), or Southern blotting and hybridization with the appropriate 32P-labeled detection oligonucleotides as described in Materials and Methods. Arrows depict the sizes of the PCR products in each case.
Mentions: Intriguingly, RT-PCR analyses (Fig. 6) of individual cell lines derived from lymphocytes, myeloid cells, monocytes, microvascular endothelial cells, and fibroblasts yielded no products in any cell type with the exception of late passage HUVEC, and placental tissue, used as a positive control. In contrast, each cell line yielded abundant PCR products when amplified with primers to CD44, or the housekeeping gene glyceraldehyde-3-phosphate dehydrogenase. These results suggested an unusually specific pattern of tissue expression for LYVE-1 that was clearly distinct from that of the CD44 molecule.

Bottom Line: Like CD44, the LYVE-1 molecule binds both soluble and immobilized HA.However, unlike CD44, the LYVE-1 molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels.Hence, LYVE-1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves.

View Article: PubMed Central - PubMed

Affiliation: University of Oxford, Molecular Immunology Group, Nuffield Department of Medicine, John Radcliff Hospital, Headington, Oxford OX3 9DU, United Kingdom.

ABSTRACT
The extracellular matrix glycosaminoglycan hyaluronan (HA) is an abundant component of skin and mesenchymal tissues where it facilitates cell migration during wound healing, inflammation, and embryonic morphogenesis. Both during normal tissue homeostasis and particularly after tissue injury, HA is mobilized from these sites through lymphatic vessels to the lymph nodes where it is degraded before entering the circulation for rapid uptake by the liver. Currently, however, the identities of HA binding molecules which control this pathway are unknown. Here we describe the first such molecule, LYVE-1, which we have identified as a major receptor for HA on the lymph vessel wall. The deduced amino acid sequence of LYVE-1 predicts a 322-residue type I integral membrane polypeptide 41% similar to the CD44 HA receptor with a 212-residue extracellular domain containing a single Link module the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE-1 molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE-1 molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE-1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves.

Show MeSH
Related in: MedlinePlus