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LYVE-1, a new homologue of the CD44 glycoprotein, is a lymph-specific receptor for hyaluronan.

Banerji S, Ni J, Wang SX, Clasper S, Su J, Tammi R, Jones M, Jackson DG - J. Cell Biol. (1999)

Bottom Line: Like CD44, the LYVE-1 molecule binds both soluble and immobilized HA.However, unlike CD44, the LYVE-1 molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels.Hence, LYVE-1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves.

View Article: PubMed Central - PubMed

Affiliation: University of Oxford, Molecular Immunology Group, Nuffield Department of Medicine, John Radcliff Hospital, Headington, Oxford OX3 9DU, United Kingdom.

ABSTRACT
The extracellular matrix glycosaminoglycan hyaluronan (HA) is an abundant component of skin and mesenchymal tissues where it facilitates cell migration during wound healing, inflammation, and embryonic morphogenesis. Both during normal tissue homeostasis and particularly after tissue injury, HA is mobilized from these sites through lymphatic vessels to the lymph nodes where it is degraded before entering the circulation for rapid uptake by the liver. Currently, however, the identities of HA binding molecules which control this pathway are unknown. Here we describe the first such molecule, LYVE-1, which we have identified as a major receptor for HA on the lymph vessel wall. The deduced amino acid sequence of LYVE-1 predicts a 322-residue type I integral membrane polypeptide 41% similar to the CD44 HA receptor with a 212-residue extracellular domain containing a single Link module the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE-1 molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE-1 molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE-1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves.

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Related in: MedlinePlus

Northern blot hybridization analysis of LYVE-1 receptor mRNA. RNA blots containing 2 μg poly(A)+ RNA per lane  from each of the tissues shown were hybridized to a 32P-labeled  full-length LYVE-1 DNA probe (top), or glyceraldehyde-3-phosphate dehydrogenase probe (bottom), and washed at high stringency before autoradiography (see Materials and Methods). The  migration positions of RNA calibration markers (kilodaltons)  are shown to the left of the figure.
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Figure 5: Northern blot hybridization analysis of LYVE-1 receptor mRNA. RNA blots containing 2 μg poly(A)+ RNA per lane from each of the tissues shown were hybridized to a 32P-labeled full-length LYVE-1 DNA probe (top), or glyceraldehyde-3-phosphate dehydrogenase probe (bottom), and washed at high stringency before autoradiography (see Materials and Methods). The migration positions of RNA calibration markers (kilodaltons) are shown to the left of the figure.

Mentions: Expression of the LYVE-1 gene in vivo was investigated by Northern blot hybridization to poly(A)+ RNAs prepared from a range of human tissues (Fig. 5). Two major bands of ∼2 and 2.6 kb were abundant in spleen, lymph node, heart, lung, and fetal liver RNA and to a lesser degree in appendix, bone marrow, placenta, muscle, and adult liver RNA. These were largely if not completely absent from peripheral blood lymphocytes, thymus, brain, kidney, and pancreas RNA. In addition, minor bands of 6–7 kb were detected to a variable extent in spleen, lymph node, fetal liver, heart, lung, and muscle. The 2.6-kb band is predicted to correspond to the LYVE-1 transcript shown in Fig. 1, whereas the 2-kb band likely represents a transcript that is polyadenylated on one or other of the consensus AATAAA motifs at positions 1819 and 1905 within the 3′ untranslated region (see Fig. 1). The minor 6–7-kb bands have not yet been characterized. Hence it is possible they represent alternatively spliced LYVE-1 transcripts.


LYVE-1, a new homologue of the CD44 glycoprotein, is a lymph-specific receptor for hyaluronan.

Banerji S, Ni J, Wang SX, Clasper S, Su J, Tammi R, Jones M, Jackson DG - J. Cell Biol. (1999)

Northern blot hybridization analysis of LYVE-1 receptor mRNA. RNA blots containing 2 μg poly(A)+ RNA per lane  from each of the tissues shown were hybridized to a 32P-labeled  full-length LYVE-1 DNA probe (top), or glyceraldehyde-3-phosphate dehydrogenase probe (bottom), and washed at high stringency before autoradiography (see Materials and Methods). The  migration positions of RNA calibration markers (kilodaltons)  are shown to the left of the figure.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2132933&req=5

Figure 5: Northern blot hybridization analysis of LYVE-1 receptor mRNA. RNA blots containing 2 μg poly(A)+ RNA per lane from each of the tissues shown were hybridized to a 32P-labeled full-length LYVE-1 DNA probe (top), or glyceraldehyde-3-phosphate dehydrogenase probe (bottom), and washed at high stringency before autoradiography (see Materials and Methods). The migration positions of RNA calibration markers (kilodaltons) are shown to the left of the figure.
Mentions: Expression of the LYVE-1 gene in vivo was investigated by Northern blot hybridization to poly(A)+ RNAs prepared from a range of human tissues (Fig. 5). Two major bands of ∼2 and 2.6 kb were abundant in spleen, lymph node, heart, lung, and fetal liver RNA and to a lesser degree in appendix, bone marrow, placenta, muscle, and adult liver RNA. These were largely if not completely absent from peripheral blood lymphocytes, thymus, brain, kidney, and pancreas RNA. In addition, minor bands of 6–7 kb were detected to a variable extent in spleen, lymph node, fetal liver, heart, lung, and muscle. The 2.6-kb band is predicted to correspond to the LYVE-1 transcript shown in Fig. 1, whereas the 2-kb band likely represents a transcript that is polyadenylated on one or other of the consensus AATAAA motifs at positions 1819 and 1905 within the 3′ untranslated region (see Fig. 1). The minor 6–7-kb bands have not yet been characterized. Hence it is possible they represent alternatively spliced LYVE-1 transcripts.

Bottom Line: Like CD44, the LYVE-1 molecule binds both soluble and immobilized HA.However, unlike CD44, the LYVE-1 molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels.Hence, LYVE-1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves.

View Article: PubMed Central - PubMed

Affiliation: University of Oxford, Molecular Immunology Group, Nuffield Department of Medicine, John Radcliff Hospital, Headington, Oxford OX3 9DU, United Kingdom.

ABSTRACT
The extracellular matrix glycosaminoglycan hyaluronan (HA) is an abundant component of skin and mesenchymal tissues where it facilitates cell migration during wound healing, inflammation, and embryonic morphogenesis. Both during normal tissue homeostasis and particularly after tissue injury, HA is mobilized from these sites through lymphatic vessels to the lymph nodes where it is degraded before entering the circulation for rapid uptake by the liver. Currently, however, the identities of HA binding molecules which control this pathway are unknown. Here we describe the first such molecule, LYVE-1, which we have identified as a major receptor for HA on the lymph vessel wall. The deduced amino acid sequence of LYVE-1 predicts a 322-residue type I integral membrane polypeptide 41% similar to the CD44 HA receptor with a 212-residue extracellular domain containing a single Link module the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE-1 molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE-1 molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE-1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves.

Show MeSH
Related in: MedlinePlus