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delta-catenin, an adhesive junction-associated protein which promotes cell scattering.

Lu Q, Paredes M, Medina M, Zhou J, Cavallo R, Peifer M, Orecchio L, Kosik KS - J. Cell Biol. (1999)

Bottom Line: We found that delta-catenin can be immunoprecipitated as a complex with other components of the adherens junction, including cadherin and beta-catenin, from transfected cells and brain.In developing mouse brain, staining with delta-catenin antibodies is prominent towards the apical boundary of the neuroepithelial cells in the ventricular zone.The Arm domain alone was sufficient for achieving localization and coimmunoprecipitation with cadherin.

View Article: PubMed Central - PubMed

Affiliation: Center for Neurologic Diseases, Department of Neurology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.

ABSTRACT
The classical adherens junction that holds epithelial cells together consists of a protein complex in which members of the cadherin family linked to various catenins are the principal components. delta-catenin is a mammalian brain protein in the Armadillo repeat superfamily with sequence similarity to the adherens junction protein p120(ctn). We found that delta-catenin can be immunoprecipitated as a complex with other components of the adherens junction, including cadherin and beta-catenin, from transfected cells and brain. The interaction with cadherin involves direct contact within the highly conserved juxtamembrane region of the COOH terminus, where p120(ctn) also binds. In developing mouse brain, staining with delta-catenin antibodies is prominent towards the apical boundary of the neuroepithelial cells in the ventricular zone. When transfected into Madin-Darby canine kidney (MDCK) epithelial cells delta-catenin colocalized with cadherin, p120(ctn), and beta-catenin. The Arm domain alone was sufficient for achieving localization and coimmunoprecipitation with cadherin. The ectopic expression of delta-catenin in MDCK cells altered their morphology, induced the elaboration of lamellipodia, interfered with monolayer formation, and increased scattering in response to hepatocyte growth factor treatment. We propose that delta-catenin can regulate adhesion molecules to implement the organization of large cellular arrays necessary for tissue morphogenesis.

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Confocal immunofluorescence microscopy of MDCK  cells transiently transfected with  δ-catenin cDNA. The cells were  double labeled with (A) δ-catenin antibodies and with (B) E-cadherin antibody. The arrow points  to the transfected cell. (C) Merged  fluorescent image showing colocalization of δ-catenin and E-cadherin. The horizontal line indicates where the XZ plane was  selected for D–F. (D–F) Respective XZ vertical sections of A–C.  Bar, 15 μm.
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Figure 4: Confocal immunofluorescence microscopy of MDCK cells transiently transfected with δ-catenin cDNA. The cells were double labeled with (A) δ-catenin antibodies and with (B) E-cadherin antibody. The arrow points to the transfected cell. (C) Merged fluorescent image showing colocalization of δ-catenin and E-cadherin. The horizontal line indicates where the XZ plane was selected for D–F. (D–F) Respective XZ vertical sections of A–C. Bar, 15 μm.

Mentions: To study the subcellular distribution of δ-catenin, both transient and stably transfected MDCK cells were analyzed by immunofluorescent confocal light microscopy. rAb62 immunostaining showed that δ-catenin was localized to the periphery of transiently transfected cells in a pattern suggestive of cell–cell junctions (Figs. 4 A and 5 A). Although less intense than the peripheral staining, labeling of the cytoplasm was above background (Figs. 4 A and 5 A) suggesting that in transfected cells, a cytoplasmic pool of δ-catenin was also present. The pattern was reminiscent of the honeycomb pattern observed in the neuroepithelium. The immunofluorescent staining was specific because rAb62 did not stain cell–cell junctions in those cells in the untransfected dish. Double labeling immunofluorescence microscopy experiments showed that δ-catenin colocalized with E-cadherin (Fig. 4, A–C) and β-catenin (data not shown).


delta-catenin, an adhesive junction-associated protein which promotes cell scattering.

Lu Q, Paredes M, Medina M, Zhou J, Cavallo R, Peifer M, Orecchio L, Kosik KS - J. Cell Biol. (1999)

Confocal immunofluorescence microscopy of MDCK  cells transiently transfected with  δ-catenin cDNA. The cells were  double labeled with (A) δ-catenin antibodies and with (B) E-cadherin antibody. The arrow points  to the transfected cell. (C) Merged  fluorescent image showing colocalization of δ-catenin and E-cadherin. The horizontal line indicates where the XZ plane was  selected for D–F. (D–F) Respective XZ vertical sections of A–C.  Bar, 15 μm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2132907&req=5

Figure 4: Confocal immunofluorescence microscopy of MDCK cells transiently transfected with δ-catenin cDNA. The cells were double labeled with (A) δ-catenin antibodies and with (B) E-cadherin antibody. The arrow points to the transfected cell. (C) Merged fluorescent image showing colocalization of δ-catenin and E-cadherin. The horizontal line indicates where the XZ plane was selected for D–F. (D–F) Respective XZ vertical sections of A–C. Bar, 15 μm.
Mentions: To study the subcellular distribution of δ-catenin, both transient and stably transfected MDCK cells were analyzed by immunofluorescent confocal light microscopy. rAb62 immunostaining showed that δ-catenin was localized to the periphery of transiently transfected cells in a pattern suggestive of cell–cell junctions (Figs. 4 A and 5 A). Although less intense than the peripheral staining, labeling of the cytoplasm was above background (Figs. 4 A and 5 A) suggesting that in transfected cells, a cytoplasmic pool of δ-catenin was also present. The pattern was reminiscent of the honeycomb pattern observed in the neuroepithelium. The immunofluorescent staining was specific because rAb62 did not stain cell–cell junctions in those cells in the untransfected dish. Double labeling immunofluorescence microscopy experiments showed that δ-catenin colocalized with E-cadherin (Fig. 4, A–C) and β-catenin (data not shown).

Bottom Line: We found that delta-catenin can be immunoprecipitated as a complex with other components of the adherens junction, including cadherin and beta-catenin, from transfected cells and brain.In developing mouse brain, staining with delta-catenin antibodies is prominent towards the apical boundary of the neuroepithelial cells in the ventricular zone.The Arm domain alone was sufficient for achieving localization and coimmunoprecipitation with cadherin.

View Article: PubMed Central - PubMed

Affiliation: Center for Neurologic Diseases, Department of Neurology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.

ABSTRACT
The classical adherens junction that holds epithelial cells together consists of a protein complex in which members of the cadherin family linked to various catenins are the principal components. delta-catenin is a mammalian brain protein in the Armadillo repeat superfamily with sequence similarity to the adherens junction protein p120(ctn). We found that delta-catenin can be immunoprecipitated as a complex with other components of the adherens junction, including cadherin and beta-catenin, from transfected cells and brain. The interaction with cadherin involves direct contact within the highly conserved juxtamembrane region of the COOH terminus, where p120(ctn) also binds. In developing mouse brain, staining with delta-catenin antibodies is prominent towards the apical boundary of the neuroepithelial cells in the ventricular zone. When transfected into Madin-Darby canine kidney (MDCK) epithelial cells delta-catenin colocalized with cadherin, p120(ctn), and beta-catenin. The Arm domain alone was sufficient for achieving localization and coimmunoprecipitation with cadherin. The ectopic expression of delta-catenin in MDCK cells altered their morphology, induced the elaboration of lamellipodia, interfered with monolayer formation, and increased scattering in response to hepatocyte growth factor treatment. We propose that delta-catenin can regulate adhesion molecules to implement the organization of large cellular arrays necessary for tissue morphogenesis.

Show MeSH
Related in: MedlinePlus