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Concerted action of Aurora B, Polo and NHK-1 kinases in centromere-specific histone 2A phosphorylation.

Brittle AL, Nanba Y, Ito T, Ohkura H - Exp. Cell Res. (2007)

Bottom Line: We found that the Aurora B kinase complex is essential for this phosphorylation at centromeres, while Polo kinase is required to down-regulate H2A phosphorylation on chromosome arms in mitosis.Epistasis analysis indicated that Polo functions upstream of the H2A kinase NHK-1 but parallel to Aurora B.Therefore, multiple mitotic kinases work together to specify the spatial and temporal pattern of H2A T119 phosphorylation.

View Article: PubMed Central - PubMed

Affiliation: The Wellcome Trust Centre for Cell Biology and Institute of Cell Biology, School of Biological Sciences, The University of Edinburgh, Edinburgh, UK.

ABSTRACT
The spatial and temporal control of histone modifications is crucial for precise regulation of chromatin structure and function. Here we report that phosphorylation of H2A at threonine 119 (T119) is enriched at centromere regions in Drosophila mitosis. We found that the Aurora B kinase complex is essential for this phosphorylation at centromeres, while Polo kinase is required to down-regulate H2A phosphorylation on chromosome arms in mitosis. Cyclin B degradation triggers loss of centromeric H2A phosphorylation at anaphase onset. Epistasis analysis indicated that Polo functions upstream of the H2A kinase NHK-1 but parallel to Aurora B. Therefore, multiple mitotic kinases work together to specify the spatial and temporal pattern of H2A T119 phosphorylation.

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H2A T119 phosphorylation on chromosome arms in the absence of Polo kinase depends on NHK-1 kinase. (A) S2 cells were immunostained using anti-dH2A-pT119 antibody after single or double depletion of NHK-1 and Polo by RNAi. (B) Average pixel intensity of anti-dH2A-pT119 immunofluorescent signals on chromosome arms. The differences between Polo and others are statistically significant (p < 0.001), while the differences between the control, NHK-1 and Polo/NHK-1 are not significant (p ?>> 0.2). The H2A phosphorylation on chromosome arms in Polo-depleted cells was dependent on NHK-1, which is known to phosphorylate H2A T119.
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fig3: H2A T119 phosphorylation on chromosome arms in the absence of Polo kinase depends on NHK-1 kinase. (A) S2 cells were immunostained using anti-dH2A-pT119 antibody after single or double depletion of NHK-1 and Polo by RNAi. (B) Average pixel intensity of anti-dH2A-pT119 immunofluorescent signals on chromosome arms. The differences between Polo and others are statistically significant (p < 0.001), while the differences between the control, NHK-1 and Polo/NHK-1 are not significant (p ?>> 0.2). The H2A phosphorylation on chromosome arms in Polo-depleted cells was dependent on NHK-1, which is known to phosphorylate H2A T119.

Mentions: Next we tested the relationship between Polo and the H2A kinase NHK-1 by co-depletion. We found that NHK-1 depletion suppresses H2A T119 phosphorylation on arms induced by a loss of Polo (Fig. 3). Quantitative analysis confirmed that the phospho-H2A signal on chromosome arms in Polo NHK-1 double depletions was decreased to a level comparable to that of the control or NHK-1 depletion.


Concerted action of Aurora B, Polo and NHK-1 kinases in centromere-specific histone 2A phosphorylation.

Brittle AL, Nanba Y, Ito T, Ohkura H - Exp. Cell Res. (2007)

H2A T119 phosphorylation on chromosome arms in the absence of Polo kinase depends on NHK-1 kinase. (A) S2 cells were immunostained using anti-dH2A-pT119 antibody after single or double depletion of NHK-1 and Polo by RNAi. (B) Average pixel intensity of anti-dH2A-pT119 immunofluorescent signals on chromosome arms. The differences between Polo and others are statistically significant (p < 0.001), while the differences between the control, NHK-1 and Polo/NHK-1 are not significant (p ?>> 0.2). The H2A phosphorylation on chromosome arms in Polo-depleted cells was dependent on NHK-1, which is known to phosphorylate H2A T119.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2131725&req=5

fig3: H2A T119 phosphorylation on chromosome arms in the absence of Polo kinase depends on NHK-1 kinase. (A) S2 cells were immunostained using anti-dH2A-pT119 antibody after single or double depletion of NHK-1 and Polo by RNAi. (B) Average pixel intensity of anti-dH2A-pT119 immunofluorescent signals on chromosome arms. The differences between Polo and others are statistically significant (p < 0.001), while the differences between the control, NHK-1 and Polo/NHK-1 are not significant (p ?>> 0.2). The H2A phosphorylation on chromosome arms in Polo-depleted cells was dependent on NHK-1, which is known to phosphorylate H2A T119.
Mentions: Next we tested the relationship between Polo and the H2A kinase NHK-1 by co-depletion. We found that NHK-1 depletion suppresses H2A T119 phosphorylation on arms induced by a loss of Polo (Fig. 3). Quantitative analysis confirmed that the phospho-H2A signal on chromosome arms in Polo NHK-1 double depletions was decreased to a level comparable to that of the control or NHK-1 depletion.

Bottom Line: We found that the Aurora B kinase complex is essential for this phosphorylation at centromeres, while Polo kinase is required to down-regulate H2A phosphorylation on chromosome arms in mitosis.Epistasis analysis indicated that Polo functions upstream of the H2A kinase NHK-1 but parallel to Aurora B.Therefore, multiple mitotic kinases work together to specify the spatial and temporal pattern of H2A T119 phosphorylation.

View Article: PubMed Central - PubMed

Affiliation: The Wellcome Trust Centre for Cell Biology and Institute of Cell Biology, School of Biological Sciences, The University of Edinburgh, Edinburgh, UK.

ABSTRACT
The spatial and temporal control of histone modifications is crucial for precise regulation of chromatin structure and function. Here we report that phosphorylation of H2A at threonine 119 (T119) is enriched at centromere regions in Drosophila mitosis. We found that the Aurora B kinase complex is essential for this phosphorylation at centromeres, while Polo kinase is required to down-regulate H2A phosphorylation on chromosome arms in mitosis. Cyclin B degradation triggers loss of centromeric H2A phosphorylation at anaphase onset. Epistasis analysis indicated that Polo functions upstream of the H2A kinase NHK-1 but parallel to Aurora B. Therefore, multiple mitotic kinases work together to specify the spatial and temporal pattern of H2A T119 phosphorylation.

Show MeSH
Related in: MedlinePlus