Limits...
Green Tea Polyphenols Reduce Gastric Epithelial Cell Proliferation and Apoptosis Stimulated by Helicobacter pylori Infection.

Akai Y, Nakajima N, Ito Y, Matsui T, Iwasaki A, Arakawa Y - J Clin Biochem Nutr (2007)

Bottom Line: Polyphenols contained in green tea and related compounds were reported to have a variety anti-tumor effects and bactericidal properties.H. pylori infection resulted in increased BrdU-labeled cells in both the antrum and the bodies.We conclude the administration with polyphenols might suppress gastric carcinogenesis that is in part related to H. pylori infection.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology & Hepatology, Nihon University School of Medicine, Tokyo 101-8309, Japan.

ABSTRACT
Recently the finding of gastric cancer in Helicobacter pylori (H. pylori)-infected mouse models was reported. Studies of humans and animal models have shown that H. pylori infection stimulates gastric epithelial cell proliferation and apoptosis. Polyphenols contained in green tea and related compounds were reported to have a variety anti-tumor effects and bactericidal properties. We studied the effect of green tea polyphenols on gastric cell proliferation and apoptosis in an H. pylori-infected mouse model. This model was prepared by inoculating Balb/c mice with 10(8) cfu of H. pylori (NCTC 11637 strain) by gavage. Beginning 18 weeks after inoculation, 0.5% polyphenols were given in drinking water every day for 2 weeks. Mice were sacrificed 1 h after bromodeoxyuridine (BrdU) was given i.p. for preparation of paraffin-embedded specimens. Cell proliferation and apoptosis were examined by the avidin-biotin complex method using anti-BrdU antibody and the TUNEL method, respectively. H. pylori infection resulted in increased BrdU-labeled cells in both the antrum and the bodies. Administration of polyphenols suppressed this increased proliferation. H. pylori infection increased apoptotic cells in both the antrum and the corpus in comparison with controls. This increase was not seen in H. pylori-infected mice given polyphenols. We conclude the administration with polyphenols might suppress gastric carcinogenesis that is in part related to H. pylori infection.

No MeSH data available.


Related in: MedlinePlus

There was no difference in the total number of cells in the crypt between the H. pylori (HP) infected group and the control. Green tea polyphenols (GTP) did not significantly change total cell number.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2127227&req=5

Figure 6: There was no difference in the total number of cells in the crypt between the H. pylori (HP) infected group and the control. Green tea polyphenols (GTP) did not significantly change total cell number.

Mentions: There was no significant difference in the number of total crypt cells between the H. pylori-inoculated group and the control. Even with GTP administration the total cell numbers were not very different (antrum: control 20.40 ± 3.66, H. pylori 20.93 ± 2.93, H. pylori + GTP 18.83 ± 2.66, bodies: control 35.08 ± 7.64, H. pylori 40.16 ± 3.88, H. pylori + GTP 36.77 ± 4.14) (Fig. 6).


Green Tea Polyphenols Reduce Gastric Epithelial Cell Proliferation and Apoptosis Stimulated by Helicobacter pylori Infection.

Akai Y, Nakajima N, Ito Y, Matsui T, Iwasaki A, Arakawa Y - J Clin Biochem Nutr (2007)

There was no difference in the total number of cells in the crypt between the H. pylori (HP) infected group and the control. Green tea polyphenols (GTP) did not significantly change total cell number.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2127227&req=5

Figure 6: There was no difference in the total number of cells in the crypt between the H. pylori (HP) infected group and the control. Green tea polyphenols (GTP) did not significantly change total cell number.
Mentions: There was no significant difference in the number of total crypt cells between the H. pylori-inoculated group and the control. Even with GTP administration the total cell numbers were not very different (antrum: control 20.40 ± 3.66, H. pylori 20.93 ± 2.93, H. pylori + GTP 18.83 ± 2.66, bodies: control 35.08 ± 7.64, H. pylori 40.16 ± 3.88, H. pylori + GTP 36.77 ± 4.14) (Fig. 6).

Bottom Line: Polyphenols contained in green tea and related compounds were reported to have a variety anti-tumor effects and bactericidal properties.H. pylori infection resulted in increased BrdU-labeled cells in both the antrum and the bodies.We conclude the administration with polyphenols might suppress gastric carcinogenesis that is in part related to H. pylori infection.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology & Hepatology, Nihon University School of Medicine, Tokyo 101-8309, Japan.

ABSTRACT
Recently the finding of gastric cancer in Helicobacter pylori (H. pylori)-infected mouse models was reported. Studies of humans and animal models have shown that H. pylori infection stimulates gastric epithelial cell proliferation and apoptosis. Polyphenols contained in green tea and related compounds were reported to have a variety anti-tumor effects and bactericidal properties. We studied the effect of green tea polyphenols on gastric cell proliferation and apoptosis in an H. pylori-infected mouse model. This model was prepared by inoculating Balb/c mice with 10(8) cfu of H. pylori (NCTC 11637 strain) by gavage. Beginning 18 weeks after inoculation, 0.5% polyphenols were given in drinking water every day for 2 weeks. Mice were sacrificed 1 h after bromodeoxyuridine (BrdU) was given i.p. for preparation of paraffin-embedded specimens. Cell proliferation and apoptosis were examined by the avidin-biotin complex method using anti-BrdU antibody and the TUNEL method, respectively. H. pylori infection resulted in increased BrdU-labeled cells in both the antrum and the bodies. Administration of polyphenols suppressed this increased proliferation. H. pylori infection increased apoptotic cells in both the antrum and the corpus in comparison with controls. This increase was not seen in H. pylori-infected mice given polyphenols. We conclude the administration with polyphenols might suppress gastric carcinogenesis that is in part related to H. pylori infection.

No MeSH data available.


Related in: MedlinePlus