Limits...
Inhibition of neutrophil activity improves cardiac function after cardiopulmonary bypass.

Abdel-Rahman U, Margraf S, Aybek T, Lögters T, Bitu-Moreno J, Francischetti I, Kranert T, Grünwald F, Windolf J, Moritz A, Scholz M - J Inflamm (Lond) (2007)

Bottom Line: LIM prevented CPB-associated increase of neutrophil counts in peripheral blood.In group II, the CI was only slightly reduced (post: 3.86 +/- 0.49; pre 4.21 +/- 1.32 l/min/m2; p = 0.23).LIM appeared to accelerate the sequestration of hyperactivated neutrophils in the spleen and to reduce neutrophil infiltration of heart and lung.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Traumatology and Hand Surgery, Heinrich-Heine University, Düsseldorf, Germany. martin.scholz@uni-duesseldorf.de.

ABSTRACT

Background: The arterial in line application of the leukocyte inhibition module (LIM) in the cardiopulmonary bypass (CPB) limits overshooting leukocyte activity during cardiac surgery. We studied in a porcine model whether LIM may have beneficial effects on cardiac function after CPB.

Methods: German landrace pigs underwent CPB (60 min myocardial ischemia; 30 min reperfusion) without (group I; n = 6) or with LIM (group II; n = 6). The cardiac indices (CI) and cardiac function were analyzed pre and post CPB with a Swan-Ganz catheter and the cardiac function analyzer. Neutrophil labeling with technetium, scintigraphy, and histological analyses were done to track activated neutrophils within the organs.

Results: LIM prevented CPB-associated increase of neutrophil counts in peripheral blood. In group I, the CI significantly declined post CPB (post: 3.26 +/- 0.31; pre: 4.05 +/- 0.45 l/min/m2; p < 0.01). In group II, the CI was only slightly reduced (post: 3.86 +/- 0.49; pre 4.21 +/- 1.32 l/min/m2; p = 0.23). Post CPB, the intergroup difference showed significantly higher CI values in the LIM group (p < 0.05) which was in conjunction with higher pre-load independent endsystolic pressure volume relationship (ESPVR) values (group I: 1.57 +/- 0.18; group II: 1.93 +/- 0.16; p < 0.001). Moreover, the systemic vascular resistance and pulmonary vascular resistance were lower in the LIM group. LIM appeared to accelerate the sequestration of hyperactivated neutrophils in the spleen and to reduce neutrophil infiltration of heart and lung.

Conclusion: Our data provides strong evidence that LIM improves perioperative hemodynamics and cardiac function after CPB by limiting neutrophil activity and inducing accelerated sequestration of neutrophils in the spleen.

No MeSH data available.


Related in: MedlinePlus

Chloroacetate esterase staining of heart and lung paraffin sections. Representative tissue samples for untreated healthy animals, animals undergoing CPB, and animals undergoing CPB with LIM. Magnification is 200-fold.
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Figure 4: Chloroacetate esterase staining of heart and lung paraffin sections. Representative tissue samples for untreated healthy animals, animals undergoing CPB, and animals undergoing CPB with LIM. Magnification is 200-fold.

Mentions: To study the possibility whether LIM may exert its beneficial effects on hemodynamics and cardiac function by reducing neutrophil tissue infiltration, tissue sections of heart and lung were stained with neutrophil specific chloracetate-esterase (Figure 4). Semi quantitative evaluation of tissue sections from CPB-treated pigs revealed neutrophil tissue infiltration in heart and lung when compared with sections from untreated control pigs. In tissue sections from LIM-treated pigs reduced numbers of neutrophils in heart and lung were found compared with the CPB group. High numbers of neutrophils were detected in the spleen of LIM-treated pigs but not in control pigs.


Inhibition of neutrophil activity improves cardiac function after cardiopulmonary bypass.

Abdel-Rahman U, Margraf S, Aybek T, Lögters T, Bitu-Moreno J, Francischetti I, Kranert T, Grünwald F, Windolf J, Moritz A, Scholz M - J Inflamm (Lond) (2007)

Chloroacetate esterase staining of heart and lung paraffin sections. Representative tissue samples for untreated healthy animals, animals undergoing CPB, and animals undergoing CPB with LIM. Magnification is 200-fold.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2100046&req=5

Figure 4: Chloroacetate esterase staining of heart and lung paraffin sections. Representative tissue samples for untreated healthy animals, animals undergoing CPB, and animals undergoing CPB with LIM. Magnification is 200-fold.
Mentions: To study the possibility whether LIM may exert its beneficial effects on hemodynamics and cardiac function by reducing neutrophil tissue infiltration, tissue sections of heart and lung were stained with neutrophil specific chloracetate-esterase (Figure 4). Semi quantitative evaluation of tissue sections from CPB-treated pigs revealed neutrophil tissue infiltration in heart and lung when compared with sections from untreated control pigs. In tissue sections from LIM-treated pigs reduced numbers of neutrophils in heart and lung were found compared with the CPB group. High numbers of neutrophils were detected in the spleen of LIM-treated pigs but not in control pigs.

Bottom Line: LIM prevented CPB-associated increase of neutrophil counts in peripheral blood.In group II, the CI was only slightly reduced (post: 3.86 +/- 0.49; pre 4.21 +/- 1.32 l/min/m2; p = 0.23).LIM appeared to accelerate the sequestration of hyperactivated neutrophils in the spleen and to reduce neutrophil infiltration of heart and lung.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Traumatology and Hand Surgery, Heinrich-Heine University, Düsseldorf, Germany. martin.scholz@uni-duesseldorf.de.

ABSTRACT

Background: The arterial in line application of the leukocyte inhibition module (LIM) in the cardiopulmonary bypass (CPB) limits overshooting leukocyte activity during cardiac surgery. We studied in a porcine model whether LIM may have beneficial effects on cardiac function after CPB.

Methods: German landrace pigs underwent CPB (60 min myocardial ischemia; 30 min reperfusion) without (group I; n = 6) or with LIM (group II; n = 6). The cardiac indices (CI) and cardiac function were analyzed pre and post CPB with a Swan-Ganz catheter and the cardiac function analyzer. Neutrophil labeling with technetium, scintigraphy, and histological analyses were done to track activated neutrophils within the organs.

Results: LIM prevented CPB-associated increase of neutrophil counts in peripheral blood. In group I, the CI significantly declined post CPB (post: 3.26 +/- 0.31; pre: 4.05 +/- 0.45 l/min/m2; p < 0.01). In group II, the CI was only slightly reduced (post: 3.86 +/- 0.49; pre 4.21 +/- 1.32 l/min/m2; p = 0.23). Post CPB, the intergroup difference showed significantly higher CI values in the LIM group (p < 0.05) which was in conjunction with higher pre-load independent endsystolic pressure volume relationship (ESPVR) values (group I: 1.57 +/- 0.18; group II: 1.93 +/- 0.16; p < 0.001). Moreover, the systemic vascular resistance and pulmonary vascular resistance were lower in the LIM group. LIM appeared to accelerate the sequestration of hyperactivated neutrophils in the spleen and to reduce neutrophil infiltration of heart and lung.

Conclusion: Our data provides strong evidence that LIM improves perioperative hemodynamics and cardiac function after CPB by limiting neutrophil activity and inducing accelerated sequestration of neutrophils in the spleen.

No MeSH data available.


Related in: MedlinePlus