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Effect of pH of amine fluoride containing toothpastes on enamel remineralization in vitro.

Arnold WH, Haase A, Hacklaender J, Gintner Z, Bánóczy J, Gaengler P - BMC Oral Health (2007)

Bottom Line: The PLM results showed a decreased porous volume of the body of the lesion after incubation with fluoridated toothpaste at pH 4.53 and 5.16.No differences between the experimental window and the control window were found in the other groups.From the results it can be concluded that slightly acidified fluoridated dentifrices may have a certain positive effect on enamel remineralization.

View Article: PubMed Central - HTML - PubMed

Affiliation: Faculty of Dental Medicine, University of Witten/Herdecke, Witten, Germany. wolfa@uni-wh.de

ABSTRACT

Background: One of the important factors of the demineralization and remineralization equilibrium of enamel is the pH of the surrounding solutions. Effort has been laid in the formulation of different fluoride compounds and the fluoride content in toothpastes but much less is known about the influence of the pH of the toothpastes on their effectiveness. It was therefore the aim of this study to investigate the influence of different pH levels on enamel remineralization in an in vitro experiment using polarization light microscopy and EDX quantitative element analysis.

Methods: A 5 x 5 mm window on the enamel surface of 40 caries free extracted human premolars was demineralized in a hydroxyethylcellulose solution at pH 4.8. The teeth were divided into 8 groups and the lower half of the window was covered with varnish serving as control. Each group was then immersed in toothpaste slurry containing amine fluoride (1400 ppm) at pH 4.1, 4.5, 5.1 and 6.9 or control toothpaste slurry without fluoride at pH 4.3, 4.7, 5.3 and 7.0. Serial sections were cut through the lesions and investigated with polarization light microscopy and quantitative EDX element analysis.

Results: The PLM results showed a decreased porous volume of the body of the lesion after incubation with fluoridated toothpaste at pH 4.53 and 5.16. No differences between the experimental window and the control window were found in the other groups. The quantitative element analysis showed no differences in the element content of any of the groups.

Conclusion: From the results it can be concluded that slightly acidified fluoridated dentifrices may have a certain positive effect on enamel remineralization.

No MeSH data available.


Related in: MedlinePlus

Quantitative distribution of the different control lesions. Quantitative distribution of lesions according to their morphological appearance in control and experimental windows after application of control toothpaste at different pH levels. The different morphologies are more or less equally distributed.
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Figure 3: Quantitative distribution of the different control lesions. Quantitative distribution of lesions according to their morphological appearance in control and experimental windows after application of control toothpaste at different pH levels. The different morphologies are more or less equally distributed.

Mentions: Morphological analysis of the sections with PLM revealed variable expression of the lesions after incubation at different pH values (Fig. 1). They were mainly expressed as inhomogeneous or homogeneous lesions, interrupted bands and as single porosities. In the experimental windows most of the lesions were expressed as single porosities and interrupted bands after treatment with fluoride Fig. 2), whereas with non fluoridated toothpastes the experimental lesions were mostly inhomogeneous or homogeneous Fig. 3). Significant differences in the lesion morphology between the control window and the experimental window were found in group 2 (pH 4.53; p = 0.032) and group 3 (pH 5.16; p = 0.014) after fluoride treatment. In the experimental windows a larger number of lesions with single porosities or interrupted bands was found than in the control windows. In all other groups no significant difference was found (p > 0.05).


Effect of pH of amine fluoride containing toothpastes on enamel remineralization in vitro.

Arnold WH, Haase A, Hacklaender J, Gintner Z, Bánóczy J, Gaengler P - BMC Oral Health (2007)

Quantitative distribution of the different control lesions. Quantitative distribution of lesions according to their morphological appearance in control and experimental windows after application of control toothpaste at different pH levels. The different morphologies are more or less equally distributed.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2099426&req=5

Figure 3: Quantitative distribution of the different control lesions. Quantitative distribution of lesions according to their morphological appearance in control and experimental windows after application of control toothpaste at different pH levels. The different morphologies are more or less equally distributed.
Mentions: Morphological analysis of the sections with PLM revealed variable expression of the lesions after incubation at different pH values (Fig. 1). They were mainly expressed as inhomogeneous or homogeneous lesions, interrupted bands and as single porosities. In the experimental windows most of the lesions were expressed as single porosities and interrupted bands after treatment with fluoride Fig. 2), whereas with non fluoridated toothpastes the experimental lesions were mostly inhomogeneous or homogeneous Fig. 3). Significant differences in the lesion morphology between the control window and the experimental window were found in group 2 (pH 4.53; p = 0.032) and group 3 (pH 5.16; p = 0.014) after fluoride treatment. In the experimental windows a larger number of lesions with single porosities or interrupted bands was found than in the control windows. In all other groups no significant difference was found (p > 0.05).

Bottom Line: The PLM results showed a decreased porous volume of the body of the lesion after incubation with fluoridated toothpaste at pH 4.53 and 5.16.No differences between the experimental window and the control window were found in the other groups.From the results it can be concluded that slightly acidified fluoridated dentifrices may have a certain positive effect on enamel remineralization.

View Article: PubMed Central - HTML - PubMed

Affiliation: Faculty of Dental Medicine, University of Witten/Herdecke, Witten, Germany. wolfa@uni-wh.de

ABSTRACT

Background: One of the important factors of the demineralization and remineralization equilibrium of enamel is the pH of the surrounding solutions. Effort has been laid in the formulation of different fluoride compounds and the fluoride content in toothpastes but much less is known about the influence of the pH of the toothpastes on their effectiveness. It was therefore the aim of this study to investigate the influence of different pH levels on enamel remineralization in an in vitro experiment using polarization light microscopy and EDX quantitative element analysis.

Methods: A 5 x 5 mm window on the enamel surface of 40 caries free extracted human premolars was demineralized in a hydroxyethylcellulose solution at pH 4.8. The teeth were divided into 8 groups and the lower half of the window was covered with varnish serving as control. Each group was then immersed in toothpaste slurry containing amine fluoride (1400 ppm) at pH 4.1, 4.5, 5.1 and 6.9 or control toothpaste slurry without fluoride at pH 4.3, 4.7, 5.3 and 7.0. Serial sections were cut through the lesions and investigated with polarization light microscopy and quantitative EDX element analysis.

Results: The PLM results showed a decreased porous volume of the body of the lesion after incubation with fluoridated toothpaste at pH 4.53 and 5.16. No differences between the experimental window and the control window were found in the other groups. The quantitative element analysis showed no differences in the element content of any of the groups.

Conclusion: From the results it can be concluded that slightly acidified fluoridated dentifrices may have a certain positive effect on enamel remineralization.

No MeSH data available.


Related in: MedlinePlus