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Decreased expression of beta1- and beta2-adrenoceptors in human diabetic atrial appendage.

Dinçer UD, Güner S, Tay A, Arioğlu E, Taşdelen A, Aşlamaci S, Bidasee KR - Cardiovasc Diabetol (2003)

Bottom Line: Using the streptozotocin-induced diabetic rat model, we have recently showed that the expression and function of beta1-adrenoreceptor were decreased in the diabetic rat heart.All diabetic patients received insulin for at least two years and had been diagnosed as diabetics for at least five years.When compared with levels in nondiabetics, steady state levels of mRNA encoding beta1-adrenoreceptor decreased by 69.2 +/- 7.6% in diabetic patients while beta2-adrenoreceptor mRNA decreased by 32.2 +/- 5.5% (p < 0.001).

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pharmacology, Faculty of Pharmacy, University of Ankara, Ankara, Turkey. dincer@pharmacy.ankara.edu.tr

ABSTRACT

Background: Using the streptozotocin-induced diabetic rat model, we have recently showed that the expression and function of beta1-adrenoreceptor were decreased in the diabetic rat heart. However, the effect of diabetes on expression of beta-adrenoreceptors in human cardiac tissue remains undefined. Therefore, the focus of the present study was to investigate the effect of diabetes on mRNA encoding beta1- and beta2-ARs in human atrial tissues.

Methods: Right atrial appendages from five diabetic (mean age 65 +/- 4.5; 4 female, 1 male) and five nondiabetic patients (mean age 56.2 +/- 2.8; 4 male, 1 female) undergoing coronary artery bypass grafting were collected and assayed using reverse transcriptase-polymerase chain reaction (RT-PCR) for their mRNA content. No patient from these two groups suffered from acute myocardial infarction and/or failure. All diabetic patients received insulin for at least two years and had been diagnosed as diabetics for at least five years.

Results: When compared with levels in nondiabetics, steady state levels of mRNA encoding beta1-adrenoreceptor decreased by 69.2 +/- 7.6% in diabetic patients while beta2-adrenoreceptor mRNA decreased by 32.2 +/- 5.5% (p < 0.001).

Conclusions: Our findings show a decreased expression of beta1- and beta2-adrenoreceptors in human diabetic atrial appendage.

No MeSH data available.


Related in: MedlinePlus

β1/β2 mRNA ratio in diabetic and non-diabetic human atrial appendages.
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Figure 3: β1/β2 mRNA ratio in diabetic and non-diabetic human atrial appendages.

Mentions: After converting mRNAs into more stable cDNAs, polymerase chain reactions were used to determine the amounts of β-AR transcripts in hearts of control and diabetic human atria. As shown in Figure 2, diabetes significantly decreases (P < 0.001) mRNA levels of β1-ARs to 69.2 ± 7.6 (Figure 1A) and β2-ARs to 32.2 ± 5.5 % (Figure 2B) of control in diabetic human atria. Also β1/β2 mRNA ratio was 67% in nondiabetic and this ratio was lowered to 43% in diabetic human atrial appendages (Figure 3). All data points were normalized to β-actin as its mRNA levels did not change significantly in this experimental paradigm (Figure 2C).


Decreased expression of beta1- and beta2-adrenoceptors in human diabetic atrial appendage.

Dinçer UD, Güner S, Tay A, Arioğlu E, Taşdelen A, Aşlamaci S, Bidasee KR - Cardiovasc Diabetol (2003)

β1/β2 mRNA ratio in diabetic and non-diabetic human atrial appendages.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC203305&req=5

Figure 3: β1/β2 mRNA ratio in diabetic and non-diabetic human atrial appendages.
Mentions: After converting mRNAs into more stable cDNAs, polymerase chain reactions were used to determine the amounts of β-AR transcripts in hearts of control and diabetic human atria. As shown in Figure 2, diabetes significantly decreases (P < 0.001) mRNA levels of β1-ARs to 69.2 ± 7.6 (Figure 1A) and β2-ARs to 32.2 ± 5.5 % (Figure 2B) of control in diabetic human atria. Also β1/β2 mRNA ratio was 67% in nondiabetic and this ratio was lowered to 43% in diabetic human atrial appendages (Figure 3). All data points were normalized to β-actin as its mRNA levels did not change significantly in this experimental paradigm (Figure 2C).

Bottom Line: Using the streptozotocin-induced diabetic rat model, we have recently showed that the expression and function of beta1-adrenoreceptor were decreased in the diabetic rat heart.All diabetic patients received insulin for at least two years and had been diagnosed as diabetics for at least five years.When compared with levels in nondiabetics, steady state levels of mRNA encoding beta1-adrenoreceptor decreased by 69.2 +/- 7.6% in diabetic patients while beta2-adrenoreceptor mRNA decreased by 32.2 +/- 5.5% (p < 0.001).

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pharmacology, Faculty of Pharmacy, University of Ankara, Ankara, Turkey. dincer@pharmacy.ankara.edu.tr

ABSTRACT

Background: Using the streptozotocin-induced diabetic rat model, we have recently showed that the expression and function of beta1-adrenoreceptor were decreased in the diabetic rat heart. However, the effect of diabetes on expression of beta-adrenoreceptors in human cardiac tissue remains undefined. Therefore, the focus of the present study was to investigate the effect of diabetes on mRNA encoding beta1- and beta2-ARs in human atrial tissues.

Methods: Right atrial appendages from five diabetic (mean age 65 +/- 4.5; 4 female, 1 male) and five nondiabetic patients (mean age 56.2 +/- 2.8; 4 male, 1 female) undergoing coronary artery bypass grafting were collected and assayed using reverse transcriptase-polymerase chain reaction (RT-PCR) for their mRNA content. No patient from these two groups suffered from acute myocardial infarction and/or failure. All diabetic patients received insulin for at least two years and had been diagnosed as diabetics for at least five years.

Results: When compared with levels in nondiabetics, steady state levels of mRNA encoding beta1-adrenoreceptor decreased by 69.2 +/- 7.6% in diabetic patients while beta2-adrenoreceptor mRNA decreased by 32.2 +/- 5.5% (p < 0.001).

Conclusions: Our findings show a decreased expression of beta1- and beta2-adrenoreceptors in human diabetic atrial appendage.

No MeSH data available.


Related in: MedlinePlus