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The mechanism of photodynamic inactivation of human cells in vitro in the presence of haematoporphyrin.

Moan J, Pettersen EO, Christensen T - Br. J. Cancer (1979)

Bottom Line: The photosensitizing effect of haematoporphyrin (HP) on human cells of the established line NHIK 3025 has been studied.Serum proteins also bind HP, and the presence of 10% human serum during incubation with HP (3 X 10(-4)M) reduces the cellular uptake of HP by 75% or more.This indicates that singlet oxygen is involved in the inactivation.

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ABSTRACT
The photosensitizing effect of haematoporphyrin (HP) on human cells of the established line NHIK 3025 has been studied. Fluorescence measurements show that HP is bound to these cells. Serum proteins also bind HP, and the presence of 10% human serum during incubation with HP (3 X 10(-4)M) reduces the cellular uptake of HP by 75% or more. The photosensitized inactivation is enhanced when the cells are suspended in D2O-buffer during irradiation. This indicates that singlet oxygen is involved in the inactivation. Two findings indicate that the photoinduced damage is repairable: firstly, the fraction of cells surviving a given light dose decreases with decreasing irradiation temperature, and secondly, the survival curves have a shoulder at low exposures of light.

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The mechanism of photodynamic inactivation of human cells in vitro in the presence of haematoporphyrin.

Moan J, Pettersen EO, Christensen T - Br. J. Cancer (1979)

© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2009925&req=5

Bottom Line: The photosensitizing effect of haematoporphyrin (HP) on human cells of the established line NHIK 3025 has been studied.Serum proteins also bind HP, and the presence of 10% human serum during incubation with HP (3 X 10(-4)M) reduces the cellular uptake of HP by 75% or more.This indicates that singlet oxygen is involved in the inactivation.

View Article: PubMed Central - PubMed

ABSTRACT
The photosensitizing effect of haematoporphyrin (HP) on human cells of the established line NHIK 3025 has been studied. Fluorescence measurements show that HP is bound to these cells. Serum proteins also bind HP, and the presence of 10% human serum during incubation with HP (3 X 10(-4)M) reduces the cellular uptake of HP by 75% or more. The photosensitized inactivation is enhanced when the cells are suspended in D2O-buffer during irradiation. This indicates that singlet oxygen is involved in the inactivation. Two findings indicate that the photoinduced damage is repairable: firstly, the fraction of cells surviving a given light dose decreases with decreasing irradiation temperature, and secondly, the survival curves have a shoulder at low exposures of light.

Show MeSH
Related in: MedlinePlus