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A soft-agar procedure measuring growth of human colonic carcinomas.

Kimball PM, Brattain MG, Pitts AM - Br. J. Cancer (1978)

Bottom Line: The effects of varying the incubation period and inoculum size upon growth were studied using unseparated cell suspensions from 6 specimens.Cluster formation appeared concentration-dependent, and individual specimens varied with respect to the inoculum most conducive to growth.The maximum plating efficiencies for unseparated cells were unseparated cells were 0.4-1.7%.

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ABSTRACT
Cell suspensions from 5 human colonic carcinomas were fractionated by velocity sedimentation and plated in soft agar. Cluster formation was restricted to the purest fraction of epithelial cells, as had been determined by immuno- and histochemical criteria. Plating efficiencies for the 5 specimens were 1.0-4.5%. The effects of varying the incubation period and inoculum size upon growth were studied using unseparated cell suspensions from 6 specimens. Clusters were apparent after 3 weeks in culture, and maximum cluster formation was typically seen by 5 weeks. Cluster formation appeared concentration-dependent, and individual specimens varied with respect to the inoculum most conducive to growth. The maximum plating efficiencies for unseparated cells were unseparated cells were 0.4-1.7%.

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Related in: MedlinePlus

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A soft-agar procedure measuring growth of human colonic carcinomas.

Kimball PM, Brattain MG, Pitts AM - Br. J. Cancer (1978)

© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2009639&req=5

Bottom Line: The effects of varying the incubation period and inoculum size upon growth were studied using unseparated cell suspensions from 6 specimens.Cluster formation appeared concentration-dependent, and individual specimens varied with respect to the inoculum most conducive to growth.The maximum plating efficiencies for unseparated cells were unseparated cells were 0.4-1.7%.

View Article: PubMed Central - PubMed

ABSTRACT
Cell suspensions from 5 human colonic carcinomas were fractionated by velocity sedimentation and plated in soft agar. Cluster formation was restricted to the purest fraction of epithelial cells, as had been determined by immuno- and histochemical criteria. Plating efficiencies for the 5 specimens were 1.0-4.5%. The effects of varying the incubation period and inoculum size upon growth were studied using unseparated cell suspensions from 6 specimens. Clusters were apparent after 3 weeks in culture, and maximum cluster formation was typically seen by 5 weeks. Cluster formation appeared concentration-dependent, and individual specimens varied with respect to the inoculum most conducive to growth. The maximum plating efficiencies for unseparated cells were unseparated cells were 0.4-1.7%.

Show MeSH
Related in: MedlinePlus