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An in vitro colony assay for human tumours grown in immune-suppressed mice and treated in vivo with cytotoxic agents.

Courtenay VD, Mills J - Br. J. Cancer (1978)

Bottom Line: The novel feature of the technique is the addition of a replenishable liquid phase which permits the maintenance of relatively slowly growing cells.The results obtained by this in vitro method are in agreement with those from the agar diffusion chamber technique.This culture method has also been successfully used for the growth of cells taken directly from human tumour biopsy samples obtained in the clinic.

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ABSTRACT
An in vitro agar colony technique has been developed for the growth of tumour cells taken directly from human tumours grown in immune-suppressed mice. The novel feature of the technique is the addition of a replenishable liquid phase which permits the maintenance of relatively slowly growing cells. A number of different xenografted tumours have been cultured successfully in this system, with red blood cells added to the agar and using 5% O2 in the gas phase. The technique has been used to assay cell survival in tumours treated in vivo with cytotoxic agents, and examples are given of survival curves obtained from a pancreatic tumours irradiated with gamma-rays and a colonic tumour from mice treated with cyclophosphamide. The results obtained by this in vitro method are in agreement with those from the agar diffusion chamber technique. This culture method has also been successfully used for the growth of cells taken directly from human tumour biopsy samples obtained in the clinic.

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Related in: MedlinePlus

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An in vitro colony assay for human tumours grown in immune-suppressed mice and treated in vivo with cytotoxic agents.

Courtenay VD, Mills J - Br. J. Cancer (1978)

© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2009601&req=5

Bottom Line: The novel feature of the technique is the addition of a replenishable liquid phase which permits the maintenance of relatively slowly growing cells.The results obtained by this in vitro method are in agreement with those from the agar diffusion chamber technique.This culture method has also been successfully used for the growth of cells taken directly from human tumour biopsy samples obtained in the clinic.

View Article: PubMed Central - PubMed

ABSTRACT
An in vitro agar colony technique has been developed for the growth of tumour cells taken directly from human tumours grown in immune-suppressed mice. The novel feature of the technique is the addition of a replenishable liquid phase which permits the maintenance of relatively slowly growing cells. A number of different xenografted tumours have been cultured successfully in this system, with red blood cells added to the agar and using 5% O2 in the gas phase. The technique has been used to assay cell survival in tumours treated in vivo with cytotoxic agents, and examples are given of survival curves obtained from a pancreatic tumours irradiated with gamma-rays and a colonic tumour from mice treated with cyclophosphamide. The results obtained by this in vitro method are in agreement with those from the agar diffusion chamber technique. This culture method has also been successfully used for the growth of cells taken directly from human tumour biopsy samples obtained in the clinic.

Show MeSH
Related in: MedlinePlus