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The use of immunoadsorbent columns for the isolation of antibodies specific for antigens associated with human bronchogenic carcinoma.

Watson RD, Smith AG, Levy JG - Br. J. Cancer (1974)

Bottom Line: An immunoadsorbent technique is described whereby tumour-specific antibodies may be isolated.Extracts from normal human lung tissue were pooled and bound to cyanogen bromide activated Sepharose 4B.Antisera raised in rabbits to a variety of extracts from human bronchogenic carcinoma were passed through these immunoadsorbent columns to yield antisera specific for tumour-associated antigens as demonstrated by immunodiffusion and immunoelectrophoresis.

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ABSTRACT
An immunoadsorbent technique is described whereby tumour-specific antibodies may be isolated. Extracts from normal human lung tissue were pooled and bound to cyanogen bromide activated Sepharose 4B. Antisera raised in rabbits to a variety of extracts from human bronchogenic carcinoma were passed through these immunoadsorbent columns to yield antisera specific for tumour-associated antigens as demonstrated by immunodiffusion and immunoelectrophoresis.

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Related in: MedlinePlus

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The use of immunoadsorbent columns for the isolation of antibodies specific for antigens associated with human bronchogenic carcinoma.

Watson RD, Smith AG, Levy JG - Br. J. Cancer (1974)

© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2009101&req=5

Bottom Line: An immunoadsorbent technique is described whereby tumour-specific antibodies may be isolated.Extracts from normal human lung tissue were pooled and bound to cyanogen bromide activated Sepharose 4B.Antisera raised in rabbits to a variety of extracts from human bronchogenic carcinoma were passed through these immunoadsorbent columns to yield antisera specific for tumour-associated antigens as demonstrated by immunodiffusion and immunoelectrophoresis.

View Article: PubMed Central - PubMed

ABSTRACT
An immunoadsorbent technique is described whereby tumour-specific antibodies may be isolated. Extracts from normal human lung tissue were pooled and bound to cyanogen bromide activated Sepharose 4B. Antisera raised in rabbits to a variety of extracts from human bronchogenic carcinoma were passed through these immunoadsorbent columns to yield antisera specific for tumour-associated antigens as demonstrated by immunodiffusion and immunoelectrophoresis.

Show MeSH
Related in: MedlinePlus