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Detection of an oestrogen receptor-like protein in human meningiomas by band shift assay using a synthetic oestrogen responsive element (ERE).

Koehorst SG, Jacobs HM, Thijssen JH, Blankenstein MA - Br. J. Cancer (1993)

Bottom Line: When a ligand binding or enzyme immunoassay is used, meningiomas are found to be rich in progestin receptors (PR) whereas oestrogen receptors (ER) are virtually undetectable.A protein that can bind to an oestrogen responsive element (ERE) was detected in meningiomas, by using the band-shift assay.The binding of ER to the ERE is inhibited by the anti-ER monoclonal antibody ER-P31, which is directed to the A/B domain of the ER, indicating that the binding protein is an ER-like protein.

View Article: PubMed Central - PubMed

Affiliation: Department of Endocrinology, Academic Hospital Utrecht, The Netherlands.

ABSTRACT
When a ligand binding or enzyme immunoassay is used, meningiomas are found to be rich in progestin receptors (PR) whereas oestrogen receptors (ER) are virtually undetectable. A protein that can bind to an oestrogen responsive element (ERE) was detected in meningiomas, by using the band-shift assay. The binding of ER to the ERE is inhibited by the anti-ER monoclonal antibody ER-P31, which is directed to the A/B domain of the ER, indicating that the binding protein is an ER-like protein.

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Related in: MedlinePlus

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Detection of an oestrogen receptor-like protein in human meningiomas by band shift assay using a synthetic oestrogen responsive element (ERE).

Koehorst SG, Jacobs HM, Thijssen JH, Blankenstein MA - Br. J. Cancer (1993)

© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC1968567&req=5

Bottom Line: When a ligand binding or enzyme immunoassay is used, meningiomas are found to be rich in progestin receptors (PR) whereas oestrogen receptors (ER) are virtually undetectable.A protein that can bind to an oestrogen responsive element (ERE) was detected in meningiomas, by using the band-shift assay.The binding of ER to the ERE is inhibited by the anti-ER monoclonal antibody ER-P31, which is directed to the A/B domain of the ER, indicating that the binding protein is an ER-like protein.

View Article: PubMed Central - PubMed

Affiliation: Department of Endocrinology, Academic Hospital Utrecht, The Netherlands.

ABSTRACT
When a ligand binding or enzyme immunoassay is used, meningiomas are found to be rich in progestin receptors (PR) whereas oestrogen receptors (ER) are virtually undetectable. A protein that can bind to an oestrogen responsive element (ERE) was detected in meningiomas, by using the band-shift assay. The binding of ER to the ERE is inhibited by the anti-ER monoclonal antibody ER-P31, which is directed to the A/B domain of the ER, indicating that the binding protein is an ER-like protein.

Show MeSH
Related in: MedlinePlus