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Genetic diversity of Leishmania amazonensis strains isolated in northeastern Brazil as revealed by DNA sequencing, PCR-based analyses and molecular karyotyping.

de Oliveira JP, Fernandes F, Cruz AK, Trombela V, Monteiro E, Camargo AA, Barral A, de Oliveira CI - Kinetoplastid Biol Dis (2007)

Bottom Line: Leishmania (Leishmania) amazonensis infection in man results in a clinical spectrum of disease manifestations ranging from cutaneous to mucosal or visceral involvement.Unrooted genetic trees separated strains into different groups.These results indicate that L. amazonensis strains isolated from leishmaniasis patients from northeastern Brazil are genetically diverse, however, no correlation between genetic polymorphism and phenotype were found.

View Article: PubMed Central - HTML - PubMed

Affiliation: Centro de Pesquisas Gonçalo Moniz, FIOCRUZ, Rua Waldemar Falcão, 121, 40296-710, Salvador, BA, Brazil. jp-ol@bol.com.br

ABSTRACT

Background: Leishmania (Leishmania) amazonensis infection in man results in a clinical spectrum of disease manifestations ranging from cutaneous to mucosal or visceral involvement. In the present study, we have investigated the genetic variability of 18 L. amazonensis strains isolated in northeastern Brazil from patients with different clinical manifestations of leishmaniasis. Parasite DNA was analyzed by sequencing of the ITS flanking the 5.8 S subunit of the ribosomal RNA genes, by RAPD and SSR-PCR and by PFGE followed by hybridization with gene-specific probes.

Results: ITS sequencing and PCR-based methods revealed genetic heterogeneity among the L. amazonensis isolates examined and molecular karyotyping also showed variation in the chromosome size of different isolates. Unrooted genetic trees separated strains into different groups.

Conclusion: These results indicate that L. amazonensis strains isolated from leishmaniasis patients from northeastern Brazil are genetically diverse, however, no correlation between genetic polymorphism and phenotype were found.

No MeSH data available.


Related in: MedlinePlus

UPGMA dendrogram based on the combined RAPD and SSR-PCR amplification patterns obtained for L. amazonensis strains. Bootstrap values above 50% are indicated.
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Figure 2: UPGMA dendrogram based on the combined RAPD and SSR-PCR amplification patterns obtained for L. amazonensis strains. Bootstrap values above 50% are indicated.

Mentions: We then compared the topology of phylogenetic tree obtained by ITS sequencing with the dendrograms generated by RAPD and SSR-PCR. Branches observed in the resulting RAPD or SSR-PCR dendrograms were not strongly supported by bootstrap analysis (data not shown). A combined analysis of RAPD and SSR-PCR results is shown in Fig. 2. The grouping of BA109 and BA112 (both obtained from VL patients) as well as the grouping of strains BA113 and BA114 (both from ML patients) were supported by significant bootstrap values (91 and 98%, respectively). In this combined analysis, BA106 (obtained from a DCL strain) also remained in a separate branch, as observed in the individuals RAPD and SSR-PCR dendrograms (data not shown). Overall, either by ITS, RAPD or SSR-PCR, we did not detect any association between genetic polymorphism and geographic origin.


Genetic diversity of Leishmania amazonensis strains isolated in northeastern Brazil as revealed by DNA sequencing, PCR-based analyses and molecular karyotyping.

de Oliveira JP, Fernandes F, Cruz AK, Trombela V, Monteiro E, Camargo AA, Barral A, de Oliveira CI - Kinetoplastid Biol Dis (2007)

UPGMA dendrogram based on the combined RAPD and SSR-PCR amplification patterns obtained for L. amazonensis strains. Bootstrap values above 50% are indicated.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC1919383&req=5

Figure 2: UPGMA dendrogram based on the combined RAPD and SSR-PCR amplification patterns obtained for L. amazonensis strains. Bootstrap values above 50% are indicated.
Mentions: We then compared the topology of phylogenetic tree obtained by ITS sequencing with the dendrograms generated by RAPD and SSR-PCR. Branches observed in the resulting RAPD or SSR-PCR dendrograms were not strongly supported by bootstrap analysis (data not shown). A combined analysis of RAPD and SSR-PCR results is shown in Fig. 2. The grouping of BA109 and BA112 (both obtained from VL patients) as well as the grouping of strains BA113 and BA114 (both from ML patients) were supported by significant bootstrap values (91 and 98%, respectively). In this combined analysis, BA106 (obtained from a DCL strain) also remained in a separate branch, as observed in the individuals RAPD and SSR-PCR dendrograms (data not shown). Overall, either by ITS, RAPD or SSR-PCR, we did not detect any association between genetic polymorphism and geographic origin.

Bottom Line: Leishmania (Leishmania) amazonensis infection in man results in a clinical spectrum of disease manifestations ranging from cutaneous to mucosal or visceral involvement.Unrooted genetic trees separated strains into different groups.These results indicate that L. amazonensis strains isolated from leishmaniasis patients from northeastern Brazil are genetically diverse, however, no correlation between genetic polymorphism and phenotype were found.

View Article: PubMed Central - HTML - PubMed

Affiliation: Centro de Pesquisas Gonçalo Moniz, FIOCRUZ, Rua Waldemar Falcão, 121, 40296-710, Salvador, BA, Brazil. jp-ol@bol.com.br

ABSTRACT

Background: Leishmania (Leishmania) amazonensis infection in man results in a clinical spectrum of disease manifestations ranging from cutaneous to mucosal or visceral involvement. In the present study, we have investigated the genetic variability of 18 L. amazonensis strains isolated in northeastern Brazil from patients with different clinical manifestations of leishmaniasis. Parasite DNA was analyzed by sequencing of the ITS flanking the 5.8 S subunit of the ribosomal RNA genes, by RAPD and SSR-PCR and by PFGE followed by hybridization with gene-specific probes.

Results: ITS sequencing and PCR-based methods revealed genetic heterogeneity among the L. amazonensis isolates examined and molecular karyotyping also showed variation in the chromosome size of different isolates. Unrooted genetic trees separated strains into different groups.

Conclusion: These results indicate that L. amazonensis strains isolated from leishmaniasis patients from northeastern Brazil are genetically diverse, however, no correlation between genetic polymorphism and phenotype were found.

No MeSH data available.


Related in: MedlinePlus