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Null effect of antidepressants on the astrocytes-mediated proliferation of hippocampal progenitor cells in vitro.

Ko HG, Lee SJ, Son H, Kaang BK - Mol Pain (2007)

Bottom Line: The increase of neurogenesis might contribute to the behavioral effects of antidepressants.In both of these systems, in comparison with naïve astrocytes, antidepressant-treated astrocytes did not further increase the proliferation of NPCs.These results suggest that astrocytes increase the proliferation of hippocampal NPCs, however, this may not be directly involved in the antidepressant-induced proliferation of NPCs.

View Article: PubMed Central - HTML - PubMed

Affiliation: National Creative Research Initiative Center for Memory and Institute of Molecular Biology and Genetics, Department of Biological Sciences, College of Natural Sciences, Seoul National University, Gwanak-gu, Seoul, Korea. dhelix2@snu.ac.kr

ABSTRACT

Background: It is well known that antidepressants increase neurogenesis in the dentate gyrus of the hippocampus. The increase of neurogenesis might contribute to the behavioral effects of antidepressants. However, the mechanism by which antidepressants increase hippocampal neurogenesis is largely unknown. It has been recently reported that astroglia induce the neurogenesis of the hippocampal neural progenitor cells (NPCs). Therefore, we hypothesized that antidepressants may act on astrocytes, and this in turn induces neurogenesis of NPCs.

Results: To examine this hypothesis, we used two co-culture systems, i.e., a contact-independent Banker culture and a contact-dependent overlay co-culture. In both of these systems, in comparison with naïve astrocytes, antidepressant-treated astrocytes did not further increase the proliferation of NPCs.

Conclusion: These results suggest that astrocytes increase the proliferation of hippocampal NPCs, however, this may not be directly involved in the antidepressant-induced proliferation of NPCs.

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Related in: MedlinePlus

Effects of antidepressant-treated astrocytes on the proliferation of NPCs in a contact-dependent culture system. (A) Proliferation of NPCs co-cultured with astrocytes using an overlay co-culture system. After treating astrocytes with antidepressants, NPCs were overlaid on the astrocytes. NPCs were labeled with GFP using a retrovirus. After BrdU immunocytochemical analysis, the nuclei were stained with DAPI (blue). An arrowhead (pink) indicates BrdU and GFP double-positive NPCs, and the arrow (pink) shows BrdU-positive cells. These BrdU-only-positive cells represent astrocytes or NPCs that were not infected with the GFP-expressing retrovirus. Scale bar, 50 μm. (B) Quantification of the proliferation of NPCs. BrdU and GFP double-positive cells were counted. In comparison with naïve astrocytes, those treated with 10 μM of desipramine or fluoxetine did not further increase the proliferation of NPCs. Data shown are mean values ± SEM. One-way ANOVA and Tukey's multiple comparison test were used for data analysis.
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Figure 3: Effects of antidepressant-treated astrocytes on the proliferation of NPCs in a contact-dependent culture system. (A) Proliferation of NPCs co-cultured with astrocytes using an overlay co-culture system. After treating astrocytes with antidepressants, NPCs were overlaid on the astrocytes. NPCs were labeled with GFP using a retrovirus. After BrdU immunocytochemical analysis, the nuclei were stained with DAPI (blue). An arrowhead (pink) indicates BrdU and GFP double-positive NPCs, and the arrow (pink) shows BrdU-positive cells. These BrdU-only-positive cells represent astrocytes or NPCs that were not infected with the GFP-expressing retrovirus. Scale bar, 50 μm. (B) Quantification of the proliferation of NPCs. BrdU and GFP double-positive cells were counted. In comparison with naïve astrocytes, those treated with 10 μM of desipramine or fluoxetine did not further increase the proliferation of NPCs. Data shown are mean values ± SEM. One-way ANOVA and Tukey's multiple comparison test were used for data analysis.

Mentions: On the other hand, the involvement of astrocytes in the proliferation of NPCs could possibly occur in a contact-dependent manner [15]. To test this possibility, we used the overlay co-culture system. In this culture system, astrocytes serve as the substrate cells for NPCs that have been labeled with GFP (Fig. 3A). As shown in Fig. 3B, in comparison with naïve astrocytes, those treated with 10 μM of desipramine or fluoxetine did not increase the number of BrdU and GFP double-positive cells (naïve: 28.5% ± 1.8%, n = 4; Dep.: 25.6% ± 0.7%, n = 5; Flx.: 23.1% ± 1.6%, n = 5, p > 0.05), indicating that desipramine- or fluoxetine-treated astrocytes may not induce proliferation of NPCs in a contact-dependent manner.


Null effect of antidepressants on the astrocytes-mediated proliferation of hippocampal progenitor cells in vitro.

Ko HG, Lee SJ, Son H, Kaang BK - Mol Pain (2007)

Effects of antidepressant-treated astrocytes on the proliferation of NPCs in a contact-dependent culture system. (A) Proliferation of NPCs co-cultured with astrocytes using an overlay co-culture system. After treating astrocytes with antidepressants, NPCs were overlaid on the astrocytes. NPCs were labeled with GFP using a retrovirus. After BrdU immunocytochemical analysis, the nuclei were stained with DAPI (blue). An arrowhead (pink) indicates BrdU and GFP double-positive NPCs, and the arrow (pink) shows BrdU-positive cells. These BrdU-only-positive cells represent astrocytes or NPCs that were not infected with the GFP-expressing retrovirus. Scale bar, 50 μm. (B) Quantification of the proliferation of NPCs. BrdU and GFP double-positive cells were counted. In comparison with naïve astrocytes, those treated with 10 μM of desipramine or fluoxetine did not further increase the proliferation of NPCs. Data shown are mean values ± SEM. One-way ANOVA and Tukey's multiple comparison test were used for data analysis.
© Copyright Policy - open-access
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC1904438&req=5

Figure 3: Effects of antidepressant-treated astrocytes on the proliferation of NPCs in a contact-dependent culture system. (A) Proliferation of NPCs co-cultured with astrocytes using an overlay co-culture system. After treating astrocytes with antidepressants, NPCs were overlaid on the astrocytes. NPCs were labeled with GFP using a retrovirus. After BrdU immunocytochemical analysis, the nuclei were stained with DAPI (blue). An arrowhead (pink) indicates BrdU and GFP double-positive NPCs, and the arrow (pink) shows BrdU-positive cells. These BrdU-only-positive cells represent astrocytes or NPCs that were not infected with the GFP-expressing retrovirus. Scale bar, 50 μm. (B) Quantification of the proliferation of NPCs. BrdU and GFP double-positive cells were counted. In comparison with naïve astrocytes, those treated with 10 μM of desipramine or fluoxetine did not further increase the proliferation of NPCs. Data shown are mean values ± SEM. One-way ANOVA and Tukey's multiple comparison test were used for data analysis.
Mentions: On the other hand, the involvement of astrocytes in the proliferation of NPCs could possibly occur in a contact-dependent manner [15]. To test this possibility, we used the overlay co-culture system. In this culture system, astrocytes serve as the substrate cells for NPCs that have been labeled with GFP (Fig. 3A). As shown in Fig. 3B, in comparison with naïve astrocytes, those treated with 10 μM of desipramine or fluoxetine did not increase the number of BrdU and GFP double-positive cells (naïve: 28.5% ± 1.8%, n = 4; Dep.: 25.6% ± 0.7%, n = 5; Flx.: 23.1% ± 1.6%, n = 5, p > 0.05), indicating that desipramine- or fluoxetine-treated astrocytes may not induce proliferation of NPCs in a contact-dependent manner.

Bottom Line: The increase of neurogenesis might contribute to the behavioral effects of antidepressants.In both of these systems, in comparison with naïve astrocytes, antidepressant-treated astrocytes did not further increase the proliferation of NPCs.These results suggest that astrocytes increase the proliferation of hippocampal NPCs, however, this may not be directly involved in the antidepressant-induced proliferation of NPCs.

View Article: PubMed Central - HTML - PubMed

Affiliation: National Creative Research Initiative Center for Memory and Institute of Molecular Biology and Genetics, Department of Biological Sciences, College of Natural Sciences, Seoul National University, Gwanak-gu, Seoul, Korea. dhelix2@snu.ac.kr

ABSTRACT

Background: It is well known that antidepressants increase neurogenesis in the dentate gyrus of the hippocampus. The increase of neurogenesis might contribute to the behavioral effects of antidepressants. However, the mechanism by which antidepressants increase hippocampal neurogenesis is largely unknown. It has been recently reported that astroglia induce the neurogenesis of the hippocampal neural progenitor cells (NPCs). Therefore, we hypothesized that antidepressants may act on astrocytes, and this in turn induces neurogenesis of NPCs.

Results: To examine this hypothesis, we used two co-culture systems, i.e., a contact-independent Banker culture and a contact-dependent overlay co-culture. In both of these systems, in comparison with naïve astrocytes, antidepressant-treated astrocytes did not further increase the proliferation of NPCs.

Conclusion: These results suggest that astrocytes increase the proliferation of hippocampal NPCs, however, this may not be directly involved in the antidepressant-induced proliferation of NPCs.

Show MeSH
Related in: MedlinePlus