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Dynamic resolution of functionally related gene sets in response to acute heat stress.

Szustakowski JD, Kosinski PA, Marrese CA, Lee JH, Elliman SJ, Nirmala N, Kemp DM - BMC Mol. Biol. (2007)

Bottom Line: Protein turnover-related pathways were significantly affected including protein folding, pre-mRNA processing, mRNA splicing, proteolysis and proteasome-related pathways.Many responses were transient, tending to normalize within 24 hours.In summary, we show that the transcriptional response to acute cell stress is largely transient and proteosome-centric.

View Article: PubMed Central - HTML - PubMed

Affiliation: Developmental and Molecular Pathways, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, USA. joseph.szustakowski@novartis.com <joseph.szustakowski@novartis.com>

ABSTRACT

Background: Using a gene clustering strategy we determined intracellular pathway relationships within skeletal myotubes in response to an acute heat stress stimuli. Following heat shock, the transcriptome was analyzed by microarray in a temporal fashion to characterize the dynamic relationship of signaling pathways.

Results: Bioinformatics analyses exposed coordination of functionally-related gene sets, depicting mechanism-based responses to heat shock. Protein turnover-related pathways were significantly affected including protein folding, pre-mRNA processing, mRNA splicing, proteolysis and proteasome-related pathways. Many responses were transient, tending to normalize within 24 hours.

Conclusion: In summary, we show that the transcriptional response to acute cell stress is largely transient and proteosome-centric.

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Related in: MedlinePlus

Dynamic regulation of the BMP gene set following heat shock. (A-E) Dotplot diagrams represent the temporal shift in mean expression levels of the BMP pathway gene set, relative to the 0 hour time point. (F), an overview of the dynamic pattern of mean expression levels of the BMP pathway gene set. Shifts to the right or left of the vertical center line represent statistical significance of directed shift (q value), rather than the mean expression level per se. The list of genes/probes that constitute the BMP pathway gene set can be found in Additional file 1.
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Figure 5: Dynamic regulation of the BMP gene set following heat shock. (A-E) Dotplot diagrams represent the temporal shift in mean expression levels of the BMP pathway gene set, relative to the 0 hour time point. (F), an overview of the dynamic pattern of mean expression levels of the BMP pathway gene set. Shifts to the right or left of the vertical center line represent statistical significance of directed shift (q value), rather than the mean expression level per se. The list of genes/probes that constitute the BMP pathway gene set can be found in Additional file 1.

Mentions: A similar analysis was carried out on the BMP signaling pathway (see Additional file 1). When traced as a function of time over the 24 hour period, the BMP signaling pathway was initially decreased at 1, 2 and 4 hours at the transcriptional level, and recovered through 8 and 24 hours post treatment (Figure 5). This result was consistent with the established role of BMP signaling in antagonizing myogenic specification during development, and in regenerating adult muscle tissue [23]. Furthermore, along with the stress response pathway and the EMP-M pathway expression traces, it appears that much of the initial response to heat shock was reversed within 24 hours of recovery.


Dynamic resolution of functionally related gene sets in response to acute heat stress.

Szustakowski JD, Kosinski PA, Marrese CA, Lee JH, Elliman SJ, Nirmala N, Kemp DM - BMC Mol. Biol. (2007)

Dynamic regulation of the BMP gene set following heat shock. (A-E) Dotplot diagrams represent the temporal shift in mean expression levels of the BMP pathway gene set, relative to the 0 hour time point. (F), an overview of the dynamic pattern of mean expression levels of the BMP pathway gene set. Shifts to the right or left of the vertical center line represent statistical significance of directed shift (q value), rather than the mean expression level per se. The list of genes/probes that constitute the BMP pathway gene set can be found in Additional file 1.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC1904231&req=5

Figure 5: Dynamic regulation of the BMP gene set following heat shock. (A-E) Dotplot diagrams represent the temporal shift in mean expression levels of the BMP pathway gene set, relative to the 0 hour time point. (F), an overview of the dynamic pattern of mean expression levels of the BMP pathway gene set. Shifts to the right or left of the vertical center line represent statistical significance of directed shift (q value), rather than the mean expression level per se. The list of genes/probes that constitute the BMP pathway gene set can be found in Additional file 1.
Mentions: A similar analysis was carried out on the BMP signaling pathway (see Additional file 1). When traced as a function of time over the 24 hour period, the BMP signaling pathway was initially decreased at 1, 2 and 4 hours at the transcriptional level, and recovered through 8 and 24 hours post treatment (Figure 5). This result was consistent with the established role of BMP signaling in antagonizing myogenic specification during development, and in regenerating adult muscle tissue [23]. Furthermore, along with the stress response pathway and the EMP-M pathway expression traces, it appears that much of the initial response to heat shock was reversed within 24 hours of recovery.

Bottom Line: Protein turnover-related pathways were significantly affected including protein folding, pre-mRNA processing, mRNA splicing, proteolysis and proteasome-related pathways.Many responses were transient, tending to normalize within 24 hours.In summary, we show that the transcriptional response to acute cell stress is largely transient and proteosome-centric.

View Article: PubMed Central - HTML - PubMed

Affiliation: Developmental and Molecular Pathways, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, USA. joseph.szustakowski@novartis.com <joseph.szustakowski@novartis.com>

ABSTRACT

Background: Using a gene clustering strategy we determined intracellular pathway relationships within skeletal myotubes in response to an acute heat stress stimuli. Following heat shock, the transcriptome was analyzed by microarray in a temporal fashion to characterize the dynamic relationship of signaling pathways.

Results: Bioinformatics analyses exposed coordination of functionally-related gene sets, depicting mechanism-based responses to heat shock. Protein turnover-related pathways were significantly affected including protein folding, pre-mRNA processing, mRNA splicing, proteolysis and proteasome-related pathways. Many responses were transient, tending to normalize within 24 hours.

Conclusion: In summary, we show that the transcriptional response to acute cell stress is largely transient and proteosome-centric.

Show MeSH
Related in: MedlinePlus