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Rearrangement and expression of immunoglobulin light chain genes can precede heavy chain expression during normal B cell development in mice.

Novobrantseva TI, Martin VM, Pelanda R, Müller W, Rajewsky K, Ehlich A - J. Exp. Med. (1999)

Bottom Line: In agreement with these earlier results, we show by a molecular single cell analysis that 4-7% of CD43(+) B cell progenitors in wild-type mice rearrange immunoglobulin (Ig)kappa genes before the assembly of a productive VHDHJH joint.Thus, mu chain expression is not a prerequisite to Igkappa light chain gene rearrangements in normal development.In addition, we show that in B cell progenitors VkappaJkappa joining rapidly initiates kappa chain expression, irrespective of the presence of a mu chain.

View Article: PubMed Central - PubMed

Affiliation: Institute for Genetics, University of Cologne,Weyertal 121, 50931 Cologne, Germany. ntanya@mac.genetik.umi-koeln.de

ABSTRACT
In mouse mutants incapable of expressing mu chains, VkappaJkappa joints are detected in the CD43(+) B cell progenitors. In agreement with these earlier results, we show by a molecular single cell analysis that 4-7% of CD43(+) B cell progenitors in wild-type mice rearrange immunoglobulin (Ig)kappa genes before the assembly of a productive VHDHJH joint. Thus, mu chain expression is not a prerequisite to Igkappa light chain gene rearrangements in normal development. Overall, approximately 15% of the total CD43(+) B cell progenitor population carry Igkappa gene rearrangements in wild-type mice. Together with the results obtained in the mouse mutants, these data fit a model in which CD43(+) progenitors rearrange IgH and Igkappa loci independently, with a seven times higher frequency in the former. In addition, we show that in B cell progenitors VkappaJkappa joining rapidly initiates kappa chain expression, irrespective of the presence of a mu chain.

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Staining of bone marrow B cell precursors for intracellular  Igκ expression. Fraction B cells from wild-type (A) and 3-83κi/+ mice  (B). Fraction D cells from wild-type (C) and 3-83κi/+ mice (D). Anti-Igκ chain antibody is shown on the y-axis, and the forward scatter of the  cells is shown on the x-axis. Fraction D cells were used to gate κ+ cells.  Numbers indicate the percentage of cells in the window.
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Figure 1: Staining of bone marrow B cell precursors for intracellular Igκ expression. Fraction B cells from wild-type (A) and 3-83κi/+ mice (B). Fraction D cells from wild-type (C) and 3-83κi/+ mice (D). Anti-Igκ chain antibody is shown on the y-axis, and the forward scatter of the cells is shown on the x-axis. Fraction D cells were used to gate κ+ cells. Numbers indicate the percentage of cells in the window.

Mentions: 3-83κi mice (21) were used at 8–12 wk of age. Wild-type mice used in the staining shown in Fig. 1 were F1 at the age of 8–12 wk from a 129sv × BALB/c cross.


Rearrangement and expression of immunoglobulin light chain genes can precede heavy chain expression during normal B cell development in mice.

Novobrantseva TI, Martin VM, Pelanda R, Müller W, Rajewsky K, Ehlich A - J. Exp. Med. (1999)

Staining of bone marrow B cell precursors for intracellular  Igκ expression. Fraction B cells from wild-type (A) and 3-83κi/+ mice  (B). Fraction D cells from wild-type (C) and 3-83κi/+ mice (D). Anti-Igκ chain antibody is shown on the y-axis, and the forward scatter of the  cells is shown on the x-axis. Fraction D cells were used to gate κ+ cells.  Numbers indicate the percentage of cells in the window.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC1887695&req=5

Figure 1: Staining of bone marrow B cell precursors for intracellular Igκ expression. Fraction B cells from wild-type (A) and 3-83κi/+ mice (B). Fraction D cells from wild-type (C) and 3-83κi/+ mice (D). Anti-Igκ chain antibody is shown on the y-axis, and the forward scatter of the cells is shown on the x-axis. Fraction D cells were used to gate κ+ cells. Numbers indicate the percentage of cells in the window.
Mentions: 3-83κi mice (21) were used at 8–12 wk of age. Wild-type mice used in the staining shown in Fig. 1 were F1 at the age of 8–12 wk from a 129sv × BALB/c cross.

Bottom Line: In agreement with these earlier results, we show by a molecular single cell analysis that 4-7% of CD43(+) B cell progenitors in wild-type mice rearrange immunoglobulin (Ig)kappa genes before the assembly of a productive VHDHJH joint.Thus, mu chain expression is not a prerequisite to Igkappa light chain gene rearrangements in normal development.In addition, we show that in B cell progenitors VkappaJkappa joining rapidly initiates kappa chain expression, irrespective of the presence of a mu chain.

View Article: PubMed Central - PubMed

Affiliation: Institute for Genetics, University of Cologne,Weyertal 121, 50931 Cologne, Germany. ntanya@mac.genetik.umi-koeln.de

ABSTRACT
In mouse mutants incapable of expressing mu chains, VkappaJkappa joints are detected in the CD43(+) B cell progenitors. In agreement with these earlier results, we show by a molecular single cell analysis that 4-7% of CD43(+) B cell progenitors in wild-type mice rearrange immunoglobulin (Ig)kappa genes before the assembly of a productive VHDHJH joint. Thus, mu chain expression is not a prerequisite to Igkappa light chain gene rearrangements in normal development. Overall, approximately 15% of the total CD43(+) B cell progenitor population carry Igkappa gene rearrangements in wild-type mice. Together with the results obtained in the mouse mutants, these data fit a model in which CD43(+) progenitors rearrange IgH and Igkappa loci independently, with a seven times higher frequency in the former. In addition, we show that in B cell progenitors VkappaJkappa joining rapidly initiates kappa chain expression, irrespective of the presence of a mu chain.

Show MeSH