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Anatomical location of macrophage migration inhibitory factor in urogenital tissues, peripheral ganglia and lumbosacral spinal cord of the rat.

Vera PL, Meyer-Siegler KL - BMC Neurosci (2003)

Bottom Line: However, some cells in the lumbosacral dorsal horn were MIF positive, GFAP negative cells suggestive of neurons.Therefore, MIF, a pro-inflammatory cytokine, is localized to pelvic organs and also in neurons of the peripheral and central nervous tissues that innervate those organs.Changes in MIF's expression at the end organ and at peripheral and central nervous system sites suggest that MIF is involved in pelvic viscera inflammation and may act at several levels to promote inflammatory changes.

View Article: PubMed Central - HTML - PubMed

Affiliation: Research & Development (151), Bay Pines VA Medical Center, Bay Pines, FL 33744, USA. pvera@hsc.usf.edu

ABSTRACT

Background: Previous work suggested that macrophage migration inhibitory factor (MIF) may be involved in bladder inflammation. Therefore, the location of MIF was determined immunohistochemically in the bladder, prostate, major pelvic ganglia, sympathetic chain, the L6-S1 dorsal root ganglia (DRG) and the lumbosacral spinal cord of the rat.

Results: In the pelvic organs, MIF immunostaining was prominent in the epithelia. MIF was widely present in neurons in the MPG and the sympathetic chain. Some of those neurons also co-localized tyrosine hydroxylase (TH). In the DRGs, some of the neurons that stained for MIF also stained for Substance P. In the lumbosacral spinal cord, MIF immunostaining was observed in the white mater, the dorsal horn, the intermediolateral region and in the area around the central canal. Many cells were intensely stained for MIF and glial fibrillary acidic protein (GFAP) suggesting they were glial cells. However, some cells in the lumbosacral dorsal horn were MIF positive, GFAP negative cells suggestive of neurons.

Conclusions: Therefore, MIF, a pro-inflammatory cytokine, is localized to pelvic organs and also in neurons of the peripheral and central nervous tissues that innervate those organs. Changes in MIF's expression at the end organ and at peripheral and central nervous system sites suggest that MIF is involved in pelvic viscera inflammation and may act at several levels to promote inflammatory changes.

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Double immunofluorescence for MIF and GFAP in the lumbosacral spinal cord. Each panel is a composite of two different photographs, arranged to overlap each other using Adobe Photoshop. A) In the dorsal funiculi, GFAP immunostaining (red) was very prominent. MIF immunostained cells (green) were seen associated with GFAP fibers. Arrow points to midline. In the dorsal horn (B & C), many of the MIF immunostained cells (green) were associated with GFAP immunofluorescence (red). However, some cells appeared to contain only MIF immunostaining (arrow). D. Several MIF immunostained cells are visible in the IML region. All of them also reveal GFAP staining. Calibration bar = 100 μm.
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Figure 4: Double immunofluorescence for MIF and GFAP in the lumbosacral spinal cord. Each panel is a composite of two different photographs, arranged to overlap each other using Adobe Photoshop. A) In the dorsal funiculi, GFAP immunostaining (red) was very prominent. MIF immunostained cells (green) were seen associated with GFAP fibers. Arrow points to midline. In the dorsal horn (B & C), many of the MIF immunostained cells (green) were associated with GFAP immunofluorescence (red). However, some cells appeared to contain only MIF immunostaining (arrow). D. Several MIF immunostained cells are visible in the IML region. All of them also reveal GFAP staining. Calibration bar = 100 μm.

Mentions: In order to establish if the MIF stained cells were neurons or glia, we employed double-immunofluorescence with MIF and GFAP. All the MIF staining cells in the white matter also showed GFAP staining (Fig 4A), as would be expected. In the dorsal horn, although the great majority of the MIF cells also localized GFAP, indicating they were glial in origin, some cells also showed only MIF staining without GFAP suggesting that MIF could also be localized in neurons (Fig 4B,4C). In the IML region, only double-labeled cells were observed, suggesting that only glial cells containing MIF were detected in the IML region (Fig 4D).


Anatomical location of macrophage migration inhibitory factor in urogenital tissues, peripheral ganglia and lumbosacral spinal cord of the rat.

Vera PL, Meyer-Siegler KL - BMC Neurosci (2003)

Double immunofluorescence for MIF and GFAP in the lumbosacral spinal cord. Each panel is a composite of two different photographs, arranged to overlap each other using Adobe Photoshop. A) In the dorsal funiculi, GFAP immunostaining (red) was very prominent. MIF immunostained cells (green) were seen associated with GFAP fibers. Arrow points to midline. In the dorsal horn (B & C), many of the MIF immunostained cells (green) were associated with GFAP immunofluorescence (red). However, some cells appeared to contain only MIF immunostaining (arrow). D. Several MIF immunostained cells are visible in the IML region. All of them also reveal GFAP staining. Calibration bar = 100 μm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC184455&req=5

Figure 4: Double immunofluorescence for MIF and GFAP in the lumbosacral spinal cord. Each panel is a composite of two different photographs, arranged to overlap each other using Adobe Photoshop. A) In the dorsal funiculi, GFAP immunostaining (red) was very prominent. MIF immunostained cells (green) were seen associated with GFAP fibers. Arrow points to midline. In the dorsal horn (B & C), many of the MIF immunostained cells (green) were associated with GFAP immunofluorescence (red). However, some cells appeared to contain only MIF immunostaining (arrow). D. Several MIF immunostained cells are visible in the IML region. All of them also reveal GFAP staining. Calibration bar = 100 μm.
Mentions: In order to establish if the MIF stained cells were neurons or glia, we employed double-immunofluorescence with MIF and GFAP. All the MIF staining cells in the white matter also showed GFAP staining (Fig 4A), as would be expected. In the dorsal horn, although the great majority of the MIF cells also localized GFAP, indicating they were glial in origin, some cells also showed only MIF staining without GFAP suggesting that MIF could also be localized in neurons (Fig 4B,4C). In the IML region, only double-labeled cells were observed, suggesting that only glial cells containing MIF were detected in the IML region (Fig 4D).

Bottom Line: However, some cells in the lumbosacral dorsal horn were MIF positive, GFAP negative cells suggestive of neurons.Therefore, MIF, a pro-inflammatory cytokine, is localized to pelvic organs and also in neurons of the peripheral and central nervous tissues that innervate those organs.Changes in MIF's expression at the end organ and at peripheral and central nervous system sites suggest that MIF is involved in pelvic viscera inflammation and may act at several levels to promote inflammatory changes.

View Article: PubMed Central - HTML - PubMed

Affiliation: Research & Development (151), Bay Pines VA Medical Center, Bay Pines, FL 33744, USA. pvera@hsc.usf.edu

ABSTRACT

Background: Previous work suggested that macrophage migration inhibitory factor (MIF) may be involved in bladder inflammation. Therefore, the location of MIF was determined immunohistochemically in the bladder, prostate, major pelvic ganglia, sympathetic chain, the L6-S1 dorsal root ganglia (DRG) and the lumbosacral spinal cord of the rat.

Results: In the pelvic organs, MIF immunostaining was prominent in the epithelia. MIF was widely present in neurons in the MPG and the sympathetic chain. Some of those neurons also co-localized tyrosine hydroxylase (TH). In the DRGs, some of the neurons that stained for MIF also stained for Substance P. In the lumbosacral spinal cord, MIF immunostaining was observed in the white mater, the dorsal horn, the intermediolateral region and in the area around the central canal. Many cells were intensely stained for MIF and glial fibrillary acidic protein (GFAP) suggesting they were glial cells. However, some cells in the lumbosacral dorsal horn were MIF positive, GFAP negative cells suggestive of neurons.

Conclusions: Therefore, MIF, a pro-inflammatory cytokine, is localized to pelvic organs and also in neurons of the peripheral and central nervous tissues that innervate those organs. Changes in MIF's expression at the end organ and at peripheral and central nervous system sites suggest that MIF is involved in pelvic viscera inflammation and may act at several levels to promote inflammatory changes.

Show MeSH
Related in: MedlinePlus