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Anatomical location of macrophage migration inhibitory factor in urogenital tissues, peripheral ganglia and lumbosacral spinal cord of the rat.

Vera PL, Meyer-Siegler KL - BMC Neurosci (2003)

Bottom Line: However, some cells in the lumbosacral dorsal horn were MIF positive, GFAP negative cells suggestive of neurons.Therefore, MIF, a pro-inflammatory cytokine, is localized to pelvic organs and also in neurons of the peripheral and central nervous tissues that innervate those organs.Changes in MIF's expression at the end organ and at peripheral and central nervous system sites suggest that MIF is involved in pelvic viscera inflammation and may act at several levels to promote inflammatory changes.

View Article: PubMed Central - HTML - PubMed

Affiliation: Research & Development (151), Bay Pines VA Medical Center, Bay Pines, FL 33744, USA. pvera@hsc.usf.edu

ABSTRACT

Background: Previous work suggested that macrophage migration inhibitory factor (MIF) may be involved in bladder inflammation. Therefore, the location of MIF was determined immunohistochemically in the bladder, prostate, major pelvic ganglia, sympathetic chain, the L6-S1 dorsal root ganglia (DRG) and the lumbosacral spinal cord of the rat.

Results: In the pelvic organs, MIF immunostaining was prominent in the epithelia. MIF was widely present in neurons in the MPG and the sympathetic chain. Some of those neurons also co-localized tyrosine hydroxylase (TH). In the DRGs, some of the neurons that stained for MIF also stained for Substance P. In the lumbosacral spinal cord, MIF immunostaining was observed in the white mater, the dorsal horn, the intermediolateral region and in the area around the central canal. Many cells were intensely stained for MIF and glial fibrillary acidic protein (GFAP) suggesting they were glial cells. However, some cells in the lumbosacral dorsal horn were MIF positive, GFAP negative cells suggestive of neurons.

Conclusions: Therefore, MIF, a pro-inflammatory cytokine, is localized to pelvic organs and also in neurons of the peripheral and central nervous tissues that innervate those organs. Changes in MIF's expression at the end organ and at peripheral and central nervous system sites suggest that MIF is involved in pelvic viscera inflammation and may act at several levels to promote inflammatory changes.

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MIF immunohistochemistry in the lumbosacral spinal cord. A) Low power view of a section processed for MIF PAP. Many intensely labeled cells are observed in the white mater, the dorsal horn and in the IML region. Diffuse staining was observed in the dorsal horn. Moderately stained cells can be seen throughout the intermediate grey and in the area around the central canal. B) Higher magnification of the area of the dorsal horn showing labeled profiles. In addition, the dorsal root entry zone (marked with an asterisk) also appears labeled with MIF. Diffuse staining over the dorsal horn is marked by the arrows. C) Weak to moderately stained cells in the around the central canal. D) MIF stained cells were also observed in the intermediolateral cell region (IML), a region that contains sacral parasympathetic preganglionic neurons and interneurons. Calibration bar, A = 400 um; B-D = 100 um.
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Figure 3: MIF immunohistochemistry in the lumbosacral spinal cord. A) Low power view of a section processed for MIF PAP. Many intensely labeled cells are observed in the white mater, the dorsal horn and in the IML region. Diffuse staining was observed in the dorsal horn. Moderately stained cells can be seen throughout the intermediate grey and in the area around the central canal. B) Higher magnification of the area of the dorsal horn showing labeled profiles. In addition, the dorsal root entry zone (marked with an asterisk) also appears labeled with MIF. Diffuse staining over the dorsal horn is marked by the arrows. C) Weak to moderately stained cells in the around the central canal. D) MIF stained cells were also observed in the intermediolateral cell region (IML), a region that contains sacral parasympathetic preganglionic neurons and interneurons. Calibration bar, A = 400 um; B-D = 100 um.

Mentions: In the L6-S1 spinal cord, many cells were immunostained with MIF antisera. Intensely stained cells were observed throughout the white matter (Fig 3A). In addition, some intensely stained and some weakly stained cells were observed in the dorsal horn (Fig 3B). The dorsal horn also showed diffuse staining that could not be associated with particular cells but was limited to the dorsal horn and did not extent to the intermediate grey matter (Fig 3B).


Anatomical location of macrophage migration inhibitory factor in urogenital tissues, peripheral ganglia and lumbosacral spinal cord of the rat.

Vera PL, Meyer-Siegler KL - BMC Neurosci (2003)

MIF immunohistochemistry in the lumbosacral spinal cord. A) Low power view of a section processed for MIF PAP. Many intensely labeled cells are observed in the white mater, the dorsal horn and in the IML region. Diffuse staining was observed in the dorsal horn. Moderately stained cells can be seen throughout the intermediate grey and in the area around the central canal. B) Higher magnification of the area of the dorsal horn showing labeled profiles. In addition, the dorsal root entry zone (marked with an asterisk) also appears labeled with MIF. Diffuse staining over the dorsal horn is marked by the arrows. C) Weak to moderately stained cells in the around the central canal. D) MIF stained cells were also observed in the intermediolateral cell region (IML), a region that contains sacral parasympathetic preganglionic neurons and interneurons. Calibration bar, A = 400 um; B-D = 100 um.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC184455&req=5

Figure 3: MIF immunohistochemistry in the lumbosacral spinal cord. A) Low power view of a section processed for MIF PAP. Many intensely labeled cells are observed in the white mater, the dorsal horn and in the IML region. Diffuse staining was observed in the dorsal horn. Moderately stained cells can be seen throughout the intermediate grey and in the area around the central canal. B) Higher magnification of the area of the dorsal horn showing labeled profiles. In addition, the dorsal root entry zone (marked with an asterisk) also appears labeled with MIF. Diffuse staining over the dorsal horn is marked by the arrows. C) Weak to moderately stained cells in the around the central canal. D) MIF stained cells were also observed in the intermediolateral cell region (IML), a region that contains sacral parasympathetic preganglionic neurons and interneurons. Calibration bar, A = 400 um; B-D = 100 um.
Mentions: In the L6-S1 spinal cord, many cells were immunostained with MIF antisera. Intensely stained cells were observed throughout the white matter (Fig 3A). In addition, some intensely stained and some weakly stained cells were observed in the dorsal horn (Fig 3B). The dorsal horn also showed diffuse staining that could not be associated with particular cells but was limited to the dorsal horn and did not extent to the intermediate grey matter (Fig 3B).

Bottom Line: However, some cells in the lumbosacral dorsal horn were MIF positive, GFAP negative cells suggestive of neurons.Therefore, MIF, a pro-inflammatory cytokine, is localized to pelvic organs and also in neurons of the peripheral and central nervous tissues that innervate those organs.Changes in MIF's expression at the end organ and at peripheral and central nervous system sites suggest that MIF is involved in pelvic viscera inflammation and may act at several levels to promote inflammatory changes.

View Article: PubMed Central - HTML - PubMed

Affiliation: Research & Development (151), Bay Pines VA Medical Center, Bay Pines, FL 33744, USA. pvera@hsc.usf.edu

ABSTRACT

Background: Previous work suggested that macrophage migration inhibitory factor (MIF) may be involved in bladder inflammation. Therefore, the location of MIF was determined immunohistochemically in the bladder, prostate, major pelvic ganglia, sympathetic chain, the L6-S1 dorsal root ganglia (DRG) and the lumbosacral spinal cord of the rat.

Results: In the pelvic organs, MIF immunostaining was prominent in the epithelia. MIF was widely present in neurons in the MPG and the sympathetic chain. Some of those neurons also co-localized tyrosine hydroxylase (TH). In the DRGs, some of the neurons that stained for MIF also stained for Substance P. In the lumbosacral spinal cord, MIF immunostaining was observed in the white mater, the dorsal horn, the intermediolateral region and in the area around the central canal. Many cells were intensely stained for MIF and glial fibrillary acidic protein (GFAP) suggesting they were glial cells. However, some cells in the lumbosacral dorsal horn were MIF positive, GFAP negative cells suggestive of neurons.

Conclusions: Therefore, MIF, a pro-inflammatory cytokine, is localized to pelvic organs and also in neurons of the peripheral and central nervous tissues that innervate those organs. Changes in MIF's expression at the end organ and at peripheral and central nervous system sites suggest that MIF is involved in pelvic viscera inflammation and may act at several levels to promote inflammatory changes.

Show MeSH
Related in: MedlinePlus