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Inter-familial relationships of the shorebirds (Aves: Charadriiformes) based on nuclear DNA sequence data.

Ericson PG, Envall I, Irestedt M, Norman JA - BMC Evol. Biol. (2003)

Bottom Line: The DNA sequence data contains a strong phylogenetic signal that results in a well-resolved phylogenetic tree with many strongly supported internodes.Taxonomically it is the most inclusive study of shorebird families that relies on nucleotide sequences.The presented phylogenetic hypothesis provides a solid framework for analyses of macroevolution of ecological, morphological and behavioural adaptations observed within the order Charadriiformes.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Vertebrate Zoology and Molecular Systematics Laboratory, Swedish Museum of Natural History, Stockholm, Sweden. per.ericson@nrm.se

ABSTRACT

Background: Phylogenetic hypotheses of higher-level relationships in the order Charadriiformes based on morphological data, partly disagree with those based on DNA-DNA hybridisation data. So far, these relationships have not been tested by analysis of DNA sequence data. Herein we utilize 1692 bp of aligned, nuclear DNA sequences obtained from 23 charadriiform species, representing 15 families. We also test earlier suggestions that bustards and sandgrouses may be nested with the charadriiforms. The data is analysed with methods based on the parsimony and maximum-likelihood criteria.

Results: Several novel phylogenetic relationships were recovered and strongly supported by the data, regardless of which method of analysis was employed. These include placing the gulls and allied groups as a sistergroup to the sandpiper-like birds, and not to the plover-like birds. The auks clearly belong to the clade with the gulls and allies, and are not basal to most other charadriiform birds as suggested in analyses of morphological data. Pluvialis, which has been supposed to belong to the plover family (Charadriidae), represents a basal branch that constitutes the sister taxon to a clade with plovers, oystercatchers and avocets. The thick-knees and sheathbills unexpectedly cluster together.

Conclusion: The DNA sequence data contains a strong phylogenetic signal that results in a well-resolved phylogenetic tree with many strongly supported internodes. Taxonomically it is the most inclusive study of shorebird families that relies on nucleotide sequences. The presented phylogenetic hypothesis provides a solid framework for analyses of macroevolution of ecological, morphological and behavioural adaptations observed within the order Charadriiformes.

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Maximum parsimony analysis Strict consensus tree calculated from the four most parsimonious trees (length 1116 steps, CI 0.71, RI 0.60) based on the combined data set.
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Figure 8: Maximum parsimony analysis Strict consensus tree calculated from the four most parsimonious trees (length 1116 steps, CI 0.71, RI 0.60) based on the combined data set.

Mentions: Parsimony and maximum-likelihood analyses of the combined sequence data yielded virtually identical tree topologies, as did the parsimony analyses of the individual genic regions (Figs. 7,8,9). The parsimony analyses yielded four most parsimonious trees (length 1128 steps, consistency index [CI] 0.71, retention index [RI] 0.60) for the combined data set, 72 most parsimonious trees (length 483, CI 0.70, RI 0.60) for RAG-1, and 76 most parsimonious trees (length 639, CI 0.72, RI 0.61) for myoglobin intron II. Bootstrap values and Bayesian support values are indicated in the trees.


Inter-familial relationships of the shorebirds (Aves: Charadriiformes) based on nuclear DNA sequence data.

Ericson PG, Envall I, Irestedt M, Norman JA - BMC Evol. Biol. (2003)

Maximum parsimony analysis Strict consensus tree calculated from the four most parsimonious trees (length 1116 steps, CI 0.71, RI 0.60) based on the combined data set.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC184354&req=5

Figure 8: Maximum parsimony analysis Strict consensus tree calculated from the four most parsimonious trees (length 1116 steps, CI 0.71, RI 0.60) based on the combined data set.
Mentions: Parsimony and maximum-likelihood analyses of the combined sequence data yielded virtually identical tree topologies, as did the parsimony analyses of the individual genic regions (Figs. 7,8,9). The parsimony analyses yielded four most parsimonious trees (length 1128 steps, consistency index [CI] 0.71, retention index [RI] 0.60) for the combined data set, 72 most parsimonious trees (length 483, CI 0.70, RI 0.60) for RAG-1, and 76 most parsimonious trees (length 639, CI 0.72, RI 0.61) for myoglobin intron II. Bootstrap values and Bayesian support values are indicated in the trees.

Bottom Line: The DNA sequence data contains a strong phylogenetic signal that results in a well-resolved phylogenetic tree with many strongly supported internodes.Taxonomically it is the most inclusive study of shorebird families that relies on nucleotide sequences.The presented phylogenetic hypothesis provides a solid framework for analyses of macroevolution of ecological, morphological and behavioural adaptations observed within the order Charadriiformes.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Vertebrate Zoology and Molecular Systematics Laboratory, Swedish Museum of Natural History, Stockholm, Sweden. per.ericson@nrm.se

ABSTRACT

Background: Phylogenetic hypotheses of higher-level relationships in the order Charadriiformes based on morphological data, partly disagree with those based on DNA-DNA hybridisation data. So far, these relationships have not been tested by analysis of DNA sequence data. Herein we utilize 1692 bp of aligned, nuclear DNA sequences obtained from 23 charadriiform species, representing 15 families. We also test earlier suggestions that bustards and sandgrouses may be nested with the charadriiforms. The data is analysed with methods based on the parsimony and maximum-likelihood criteria.

Results: Several novel phylogenetic relationships were recovered and strongly supported by the data, regardless of which method of analysis was employed. These include placing the gulls and allied groups as a sistergroup to the sandpiper-like birds, and not to the plover-like birds. The auks clearly belong to the clade with the gulls and allies, and are not basal to most other charadriiform birds as suggested in analyses of morphological data. Pluvialis, which has been supposed to belong to the plover family (Charadriidae), represents a basal branch that constitutes the sister taxon to a clade with plovers, oystercatchers and avocets. The thick-knees and sheathbills unexpectedly cluster together.

Conclusion: The DNA sequence data contains a strong phylogenetic signal that results in a well-resolved phylogenetic tree with many strongly supported internodes. Taxonomically it is the most inclusive study of shorebird families that relies on nucleotide sequences. The presented phylogenetic hypothesis provides a solid framework for analyses of macroevolution of ecological, morphological and behavioural adaptations observed within the order Charadriiformes.

Show MeSH