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Inter-familial relationships of the shorebirds (Aves: Charadriiformes) based on nuclear DNA sequence data.

Ericson PG, Envall I, Irestedt M, Norman JA - BMC Evol. Biol. (2003)

Bottom Line: The DNA sequence data contains a strong phylogenetic signal that results in a well-resolved phylogenetic tree with many strongly supported internodes.Taxonomically it is the most inclusive study of shorebird families that relies on nucleotide sequences.The presented phylogenetic hypothesis provides a solid framework for analyses of macroevolution of ecological, morphological and behavioural adaptations observed within the order Charadriiformes.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Vertebrate Zoology and Molecular Systematics Laboratory, Swedish Museum of Natural History, Stockholm, Sweden. per.ericson@nrm.se

ABSTRACT

Background: Phylogenetic hypotheses of higher-level relationships in the order Charadriiformes based on morphological data, partly disagree with those based on DNA-DNA hybridisation data. So far, these relationships have not been tested by analysis of DNA sequence data. Herein we utilize 1692 bp of aligned, nuclear DNA sequences obtained from 23 charadriiform species, representing 15 families. We also test earlier suggestions that bustards and sandgrouses may be nested with the charadriiforms. The data is analysed with methods based on the parsimony and maximum-likelihood criteria.

Results: Several novel phylogenetic relationships were recovered and strongly supported by the data, regardless of which method of analysis was employed. These include placing the gulls and allied groups as a sistergroup to the sandpiper-like birds, and not to the plover-like birds. The auks clearly belong to the clade with the gulls and allies, and are not basal to most other charadriiform birds as suggested in analyses of morphological data. Pluvialis, which has been supposed to belong to the plover family (Charadriidae), represents a basal branch that constitutes the sister taxon to a clade with plovers, oystercatchers and avocets. The thick-knees and sheathbills unexpectedly cluster together.

Conclusion: The DNA sequence data contains a strong phylogenetic signal that results in a well-resolved phylogenetic tree with many strongly supported internodes. Taxonomically it is the most inclusive study of shorebird families that relies on nucleotide sequences. The presented phylogenetic hypothesis provides a solid framework for analyses of macroevolution of ecological, morphological and behavioural adaptations observed within the order Charadriiformes.

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Comparison between pairwise sequence divergences in RAG-1 and myoglobin intron II Solid circles indicate observed pairwise distances observed within the charadriiforms; open circles indicate distances between the charadriiform taxa and the bustard, crane, pigeon and sandgrouse; and triangles indicate distances between charadriiforms and the anseriform and galliform outgroup.
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Figure 6: Comparison between pairwise sequence divergences in RAG-1 and myoglobin intron II Solid circles indicate observed pairwise distances observed within the charadriiforms; open circles indicate distances between the charadriiform taxa and the bustard, crane, pigeon and sandgrouse; and triangles indicate distances between charadriiforms and the anseriform and galliform outgroup.

Mentions: Neither RAG-1, nor myoglobin intron II, showed any sign of saturation with both transitions and transversions linearly related to the observed pairwise sequence distances, with no obvious tendency to level off (Figs. 4,5). A plot of the pairwise, uncorrected sequence distances observed in RAG-1 and myoglobin intron II, respectively, showed an almost linear relationship between the two genic regions (Fig. 6). The slope of the regression line estimated to fit these data indicates that the myoglobin intron II evolves at a rate that is about 60% faster than that in RAG-1.


Inter-familial relationships of the shorebirds (Aves: Charadriiformes) based on nuclear DNA sequence data.

Ericson PG, Envall I, Irestedt M, Norman JA - BMC Evol. Biol. (2003)

Comparison between pairwise sequence divergences in RAG-1 and myoglobin intron II Solid circles indicate observed pairwise distances observed within the charadriiforms; open circles indicate distances between the charadriiform taxa and the bustard, crane, pigeon and sandgrouse; and triangles indicate distances between charadriiforms and the anseriform and galliform outgroup.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC184354&req=5

Figure 6: Comparison between pairwise sequence divergences in RAG-1 and myoglobin intron II Solid circles indicate observed pairwise distances observed within the charadriiforms; open circles indicate distances between the charadriiform taxa and the bustard, crane, pigeon and sandgrouse; and triangles indicate distances between charadriiforms and the anseriform and galliform outgroup.
Mentions: Neither RAG-1, nor myoglobin intron II, showed any sign of saturation with both transitions and transversions linearly related to the observed pairwise sequence distances, with no obvious tendency to level off (Figs. 4,5). A plot of the pairwise, uncorrected sequence distances observed in RAG-1 and myoglobin intron II, respectively, showed an almost linear relationship between the two genic regions (Fig. 6). The slope of the regression line estimated to fit these data indicates that the myoglobin intron II evolves at a rate that is about 60% faster than that in RAG-1.

Bottom Line: The DNA sequence data contains a strong phylogenetic signal that results in a well-resolved phylogenetic tree with many strongly supported internodes.Taxonomically it is the most inclusive study of shorebird families that relies on nucleotide sequences.The presented phylogenetic hypothesis provides a solid framework for analyses of macroevolution of ecological, morphological and behavioural adaptations observed within the order Charadriiformes.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Vertebrate Zoology and Molecular Systematics Laboratory, Swedish Museum of Natural History, Stockholm, Sweden. per.ericson@nrm.se

ABSTRACT

Background: Phylogenetic hypotheses of higher-level relationships in the order Charadriiformes based on morphological data, partly disagree with those based on DNA-DNA hybridisation data. So far, these relationships have not been tested by analysis of DNA sequence data. Herein we utilize 1692 bp of aligned, nuclear DNA sequences obtained from 23 charadriiform species, representing 15 families. We also test earlier suggestions that bustards and sandgrouses may be nested with the charadriiforms. The data is analysed with methods based on the parsimony and maximum-likelihood criteria.

Results: Several novel phylogenetic relationships were recovered and strongly supported by the data, regardless of which method of analysis was employed. These include placing the gulls and allied groups as a sistergroup to the sandpiper-like birds, and not to the plover-like birds. The auks clearly belong to the clade with the gulls and allies, and are not basal to most other charadriiform birds as suggested in analyses of morphological data. Pluvialis, which has been supposed to belong to the plover family (Charadriidae), represents a basal branch that constitutes the sister taxon to a clade with plovers, oystercatchers and avocets. The thick-knees and sheathbills unexpectedly cluster together.

Conclusion: The DNA sequence data contains a strong phylogenetic signal that results in a well-resolved phylogenetic tree with many strongly supported internodes. Taxonomically it is the most inclusive study of shorebird families that relies on nucleotide sequences. The presented phylogenetic hypothesis provides a solid framework for analyses of macroevolution of ecological, morphological and behavioural adaptations observed within the order Charadriiformes.

Show MeSH