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Measuring the lung function in the mouse: the challenge of size.

Irvin CG, Bates JH - Respir. Res. (2003)

Bottom Line: Measurement of the effects of drugs, mediators and infectious agents on various models of lung disease, as well as assessment of lung function in the intact mouse has the potential for significantly advancing our knowledge of lung disease.Because of compromises made between precision and noninvasiveness, data obtained may have an uncertain bearing on the mechanical response of the lung.These advances, resulting in our current ability to measure sophisticated indices of lung function in laboratory animals, are likely to lead to important insights into the mechanisms of lung disease.

View Article: PubMed Central - HTML - PubMed

Affiliation: Vermont Lung Center, Department of Medicine, College of Medicine, University of Vermont, Room 226, HSRF, 149 Beaumont Avenue, Burlington, VT 05405, USA. Charles.Irvin@uvm.edu

ABSTRACT
Measurement of the effects of drugs, mediators and infectious agents on various models of lung disease, as well as assessment of lung function in the intact mouse has the potential for significantly advancing our knowledge of lung disease. However, the small size of the mouse presents significant challenges for the assessment of lung function. Because of compromises made between precision and noninvasiveness, data obtained may have an uncertain bearing on the mechanical response of the lung. Nevertheless, considerable recent progress has been made in developing valid and useful measures of mouse lung function. These advances, resulting in our current ability to measure sophisticated indices of lung function in laboratory animals, are likely to lead to important insights into the mechanisms of lung disease.

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Pulmonary impedance measurements in anesthetized mice. The diagram shows airway opening pressure plotted against time. Volume and flow excursions for each breath are maintained constant by use of a volume-cycled ventilator. Increases in the magnitude of lung impedance following acethycholine injection are assessed as the increase in pressure above baseline. Note the increased responsiveness in the A/J compared the C3He/J strain of mouse. APTI: Airway pressure time index. Used with permission [38].
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Figure 4: Pulmonary impedance measurements in anesthetized mice. The diagram shows airway opening pressure plotted against time. Volume and flow excursions for each breath are maintained constant by use of a volume-cycled ventilator. Increases in the magnitude of lung impedance following acethycholine injection are assessed as the increase in pressure above baseline. Note the increased responsiveness in the A/J compared the C3He/J strain of mouse. APTI: Airway pressure time index. Used with permission [38].

Mentions: As seen in the example in Figure 4, pressure is obtained by placing a pressure transducer at the airway opening, while flow is assumed to be constant as the animal is mechanically ventilated with a volume-cycled ventilator [9,10,37,38]. When a bronchoactive agent is introduced, the peak pressure with each breath goes up, so /Zrs/ increases commensurately. Hence, by merely measuring airway-opening pressure, a useful index of lung function is derived. This technique has been used because it is simple and gives a direct assessment of lung mechanics [9,10,37,38].


Measuring the lung function in the mouse: the challenge of size.

Irvin CG, Bates JH - Respir. Res. (2003)

Pulmonary impedance measurements in anesthetized mice. The diagram shows airway opening pressure plotted against time. Volume and flow excursions for each breath are maintained constant by use of a volume-cycled ventilator. Increases in the magnitude of lung impedance following acethycholine injection are assessed as the increase in pressure above baseline. Note the increased responsiveness in the A/J compared the C3He/J strain of mouse. APTI: Airway pressure time index. Used with permission [38].
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC184039&req=5

Figure 4: Pulmonary impedance measurements in anesthetized mice. The diagram shows airway opening pressure plotted against time. Volume and flow excursions for each breath are maintained constant by use of a volume-cycled ventilator. Increases in the magnitude of lung impedance following acethycholine injection are assessed as the increase in pressure above baseline. Note the increased responsiveness in the A/J compared the C3He/J strain of mouse. APTI: Airway pressure time index. Used with permission [38].
Mentions: As seen in the example in Figure 4, pressure is obtained by placing a pressure transducer at the airway opening, while flow is assumed to be constant as the animal is mechanically ventilated with a volume-cycled ventilator [9,10,37,38]. When a bronchoactive agent is introduced, the peak pressure with each breath goes up, so /Zrs/ increases commensurately. Hence, by merely measuring airway-opening pressure, a useful index of lung function is derived. This technique has been used because it is simple and gives a direct assessment of lung mechanics [9,10,37,38].

Bottom Line: Measurement of the effects of drugs, mediators and infectious agents on various models of lung disease, as well as assessment of lung function in the intact mouse has the potential for significantly advancing our knowledge of lung disease.Because of compromises made between precision and noninvasiveness, data obtained may have an uncertain bearing on the mechanical response of the lung.These advances, resulting in our current ability to measure sophisticated indices of lung function in laboratory animals, are likely to lead to important insights into the mechanisms of lung disease.

View Article: PubMed Central - HTML - PubMed

Affiliation: Vermont Lung Center, Department of Medicine, College of Medicine, University of Vermont, Room 226, HSRF, 149 Beaumont Avenue, Burlington, VT 05405, USA. Charles.Irvin@uvm.edu

ABSTRACT
Measurement of the effects of drugs, mediators and infectious agents on various models of lung disease, as well as assessment of lung function in the intact mouse has the potential for significantly advancing our knowledge of lung disease. However, the small size of the mouse presents significant challenges for the assessment of lung function. Because of compromises made between precision and noninvasiveness, data obtained may have an uncertain bearing on the mechanical response of the lung. Nevertheless, considerable recent progress has been made in developing valid and useful measures of mouse lung function. These advances, resulting in our current ability to measure sophisticated indices of lung function in laboratory animals, are likely to lead to important insights into the mechanisms of lung disease.

Show MeSH
Related in: MedlinePlus