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Lysophospholipid receptors are differentially expressed in rat terminal Schwann cells, as revealed by a single cell rt-PCR and in situ hybridization.

Kobashi H, Yaoi T, Oda R, Okajima S, Fujiwara H, Kubo T, Fushiki S - Acta Histochem Cytochem (2006)

Bottom Line: It turned out that LPA3 was expressed only in TSCs, whereas S1P1 was expressed in TSCs and skeletal muscle, but not in MSCs.Other types of LPL receptor genes, including LPA1, S1P2, S1P3, S1P4, were expressed in both types of Schwann cells.None of the LPL receptor gene family showed MSCs-specific expression.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology and Applied Neurobiology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine,Kawaramachi-Hirokoji, Kyoto 602-8566, Japan.

ABSTRACT
Terminal Schwann cells (TSCs) that cover motor neuron terminals, are known to play an important role in maintaining neuromuscular junctions, as well as in the repair process after nerve injury. However, the molecular characteristics of TSCs remain unknown, because of the difficulties in analyzing them due to their paucity. By using our previously reported method of selectively and efficiently collecting TSCs, we have analyzed the difference in expression patterns of lysophospholipid (LPL) receptor genes (LPA1, LPA2, LPA3, S1P1, S1P2, S1P3, S1P4, and S1P5) between TSCs and myelinating Schwann cells (MSCs). LPL, which includes lysophosphatidic acid (LPA) and sphingosine 1-phosphate (S1P), is the bioactive lipid that induces a myriad of cellular responses through specific members of G-protein coupled receptors for LPA. It turned out that LPA3 was expressed only in TSCs, whereas S1P1 was expressed in TSCs and skeletal muscle, but not in MSCs. Other types of LPL receptor genes, including LPA1, S1P2, S1P3, S1P4, were expressed in both types of Schwann cells. None of the LPL receptor gene family showed MSCs-specific expression.

No MeSH data available.


Related in: MedlinePlus

In situ hybridization in adult rat soleus using LPA3 sense cRNA probe as a negative control (A) and LPA3 antisense cRNA probe (B–D). Cell bodies of terminal Schwann cells on the rat soleus were stained (black arrowheads in B, C, D). Unstained axons were visualized under a differential interference contrast microscope (black arrows). D is a higher magnification image of the area indicated by a rectangle in C. Longitudinal and horizontal stripes were muscle fibers. Bar=50 µm (A), 20 µm (B), 40 µm (C), and 10 µm (D).
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Figure 2: In situ hybridization in adult rat soleus using LPA3 sense cRNA probe as a negative control (A) and LPA3 antisense cRNA probe (B–D). Cell bodies of terminal Schwann cells on the rat soleus were stained (black arrowheads in B, C, D). Unstained axons were visualized under a differential interference contrast microscope (black arrows). D is a higher magnification image of the area indicated by a rectangle in C. Longitudinal and horizontal stripes were muscle fibers. Bar=50 µm (A), 20 µm (B), 40 µm (C), and 10 µm (D).

Mentions: To confirm whether LPA3 was expressed selectively in TSCs, we have performed in situ hybridization in NMJs of adult rat soleus. In situ hybridization was carried out for the longitudinal sections of adult rat soleus muscle, according to a whole mount in situ hybridization protocol. With antisense probe, strong signals were detected specifically in the cell bodies located on top of motor nerve terminals, but not in nerve terminals themselves, axons, or postsynaptic areas (Fig. 2B, C). No signal was detected using sense probe as a negative control (Fig. 2A).


Lysophospholipid receptors are differentially expressed in rat terminal Schwann cells, as revealed by a single cell rt-PCR and in situ hybridization.

Kobashi H, Yaoi T, Oda R, Okajima S, Fujiwara H, Kubo T, Fushiki S - Acta Histochem Cytochem (2006)

In situ hybridization in adult rat soleus using LPA3 sense cRNA probe as a negative control (A) and LPA3 antisense cRNA probe (B–D). Cell bodies of terminal Schwann cells on the rat soleus were stained (black arrowheads in B, C, D). Unstained axons were visualized under a differential interference contrast microscope (black arrows). D is a higher magnification image of the area indicated by a rectangle in C. Longitudinal and horizontal stripes were muscle fibers. Bar=50 µm (A), 20 µm (B), 40 µm (C), and 10 µm (D).
© Copyright Policy
Related In: Results  -  Collection

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Figure 2: In situ hybridization in adult rat soleus using LPA3 sense cRNA probe as a negative control (A) and LPA3 antisense cRNA probe (B–D). Cell bodies of terminal Schwann cells on the rat soleus were stained (black arrowheads in B, C, D). Unstained axons were visualized under a differential interference contrast microscope (black arrows). D is a higher magnification image of the area indicated by a rectangle in C. Longitudinal and horizontal stripes were muscle fibers. Bar=50 µm (A), 20 µm (B), 40 µm (C), and 10 µm (D).
Mentions: To confirm whether LPA3 was expressed selectively in TSCs, we have performed in situ hybridization in NMJs of adult rat soleus. In situ hybridization was carried out for the longitudinal sections of adult rat soleus muscle, according to a whole mount in situ hybridization protocol. With antisense probe, strong signals were detected specifically in the cell bodies located on top of motor nerve terminals, but not in nerve terminals themselves, axons, or postsynaptic areas (Fig. 2B, C). No signal was detected using sense probe as a negative control (Fig. 2A).

Bottom Line: It turned out that LPA3 was expressed only in TSCs, whereas S1P1 was expressed in TSCs and skeletal muscle, but not in MSCs.Other types of LPL receptor genes, including LPA1, S1P2, S1P3, S1P4, were expressed in both types of Schwann cells.None of the LPL receptor gene family showed MSCs-specific expression.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology and Applied Neurobiology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine,Kawaramachi-Hirokoji, Kyoto 602-8566, Japan.

ABSTRACT
Terminal Schwann cells (TSCs) that cover motor neuron terminals, are known to play an important role in maintaining neuromuscular junctions, as well as in the repair process after nerve injury. However, the molecular characteristics of TSCs remain unknown, because of the difficulties in analyzing them due to their paucity. By using our previously reported method of selectively and efficiently collecting TSCs, we have analyzed the difference in expression patterns of lysophospholipid (LPL) receptor genes (LPA1, LPA2, LPA3, S1P1, S1P2, S1P3, S1P4, and S1P5) between TSCs and myelinating Schwann cells (MSCs). LPL, which includes lysophosphatidic acid (LPA) and sphingosine 1-phosphate (S1P), is the bioactive lipid that induces a myriad of cellular responses through specific members of G-protein coupled receptors for LPA. It turned out that LPA3 was expressed only in TSCs, whereas S1P1 was expressed in TSCs and skeletal muscle, but not in MSCs. Other types of LPL receptor genes, including LPA1, S1P2, S1P3, S1P4, were expressed in both types of Schwann cells. None of the LPL receptor gene family showed MSCs-specific expression.

No MeSH data available.


Related in: MedlinePlus