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Localization of estrogen receptors alpha and beta in the articular surface of the rat femur.

Oshima Y, Matsuda K, Yoshida A, Watanabe N, Kawata M, Kubo T - Acta Histochem Cytochem (2007)

Bottom Line: Although many investigators have reported the presence of the estrogen receptors (ERs) alpha and beta in the articular cartilage, the localization of these receptors and the difference in their in vivo expression have not yet been clearly demonstrated.We performed immunofluorescence staining of ERalpha and ERbeta to elucidate the localization of the ERs and to note the effects of gender and the aging process on these receptors.Therefore, this study suggests the direct effects of estrogen and ER expression on articular surface metabolism.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedics, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan.

ABSTRACT
It has been suggested that the degradation of the articular cartilage and osteoarthritis (OA) are associated with gender and the estrogen hormone. Although many investigators have reported the presence of the estrogen receptors (ERs) alpha and beta in the articular cartilage, the localization of these receptors and the difference in their in vivo expression have not yet been clearly demonstrated. We performed immunofluorescence staining of ERalpha and ERbeta to elucidate the localization of the ERs and to note the effects of gender and the aging process on these receptors. The results revealed that ERalpha and ERbeta were expressed in the articular cartilage and subchondral bone layers of adult rats of both sexes. We also observed the high expression of these receptors in immature rats. In contrast, their expression levels decreased in an ovariectomised model, as a simulation of postmenopause, and in aged female rats. Therefore, this study suggests the direct effects of estrogen and ER expression on articular surface metabolism.

No MeSH data available.


Related in: MedlinePlus

1-month-old female rat. Articular surface was constructed with articular cartilage and subchondral bone layers (A). Almost all of the cells in both the articular cartilage and subchondral bone layers were stained with ERα (B) and ERβ (C). Bar=50 µm.
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Figure 3: 1-month-old female rat. Articular surface was constructed with articular cartilage and subchondral bone layers (A). Almost all of the cells in both the articular cartilage and subchondral bone layers were stained with ERα (B) and ERβ (C). Bar=50 µm.

Mentions: Endochondral ossification was started, and subchondral bone had formed; however, articular cartilage was not well developed at this time interval (Fig. 3A). Almost all of the cells in both the articular cartilage and subchondral bone layers were stained with ERα and ERβ. The strength of the ERα signal was similar to that of ERβ (Fig. 3B, C, Table 1).


Localization of estrogen receptors alpha and beta in the articular surface of the rat femur.

Oshima Y, Matsuda K, Yoshida A, Watanabe N, Kawata M, Kubo T - Acta Histochem Cytochem (2007)

1-month-old female rat. Articular surface was constructed with articular cartilage and subchondral bone layers (A). Almost all of the cells in both the articular cartilage and subchondral bone layers were stained with ERα (B) and ERβ (C). Bar=50 µm.
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Related In: Results  -  Collection

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Figure 3: 1-month-old female rat. Articular surface was constructed with articular cartilage and subchondral bone layers (A). Almost all of the cells in both the articular cartilage and subchondral bone layers were stained with ERα (B) and ERβ (C). Bar=50 µm.
Mentions: Endochondral ossification was started, and subchondral bone had formed; however, articular cartilage was not well developed at this time interval (Fig. 3A). Almost all of the cells in both the articular cartilage and subchondral bone layers were stained with ERα and ERβ. The strength of the ERα signal was similar to that of ERβ (Fig. 3B, C, Table 1).

Bottom Line: Although many investigators have reported the presence of the estrogen receptors (ERs) alpha and beta in the articular cartilage, the localization of these receptors and the difference in their in vivo expression have not yet been clearly demonstrated.We performed immunofluorescence staining of ERalpha and ERbeta to elucidate the localization of the ERs and to note the effects of gender and the aging process on these receptors.Therefore, this study suggests the direct effects of estrogen and ER expression on articular surface metabolism.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedics, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan.

ABSTRACT
It has been suggested that the degradation of the articular cartilage and osteoarthritis (OA) are associated with gender and the estrogen hormone. Although many investigators have reported the presence of the estrogen receptors (ERs) alpha and beta in the articular cartilage, the localization of these receptors and the difference in their in vivo expression have not yet been clearly demonstrated. We performed immunofluorescence staining of ERalpha and ERbeta to elucidate the localization of the ERs and to note the effects of gender and the aging process on these receptors. The results revealed that ERalpha and ERbeta were expressed in the articular cartilage and subchondral bone layers of adult rats of both sexes. We also observed the high expression of these receptors in immature rats. In contrast, their expression levels decreased in an ovariectomised model, as a simulation of postmenopause, and in aged female rats. Therefore, this study suggests the direct effects of estrogen and ER expression on articular surface metabolism.

No MeSH data available.


Related in: MedlinePlus