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Spatial codes in dendritic BC1 RNA.

Muslimov IA, Iacoangeli A, Brosius J, Tiedge H - J. Cell Biol. (2006)

Bottom Line: This element features a GA kink-turn (KT) motif that is indispensable for distal targeting.It specifically interacts with heterogeneous nuclear ribonucleoprotein A2, a trans-acting targeting factor that has previously been implicated in the transport of MBP mRNA in oligodendrocytes and neurons.Our work suggests that a BC1 KT motif encodes distal targeting via the A2 pathway and that architectural RNA elements, such as KT motifs, may function as spatial codes in neural cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology and Pharmacology, The Robert F. Furchgott Center for Neural and Behavioral Science, State University of New York Health Science Center at Brooklyn, Brooklyn, NY 11203, USA.

ABSTRACT
BC1 RNA is a dendritic untranslated RNA that has been implicated in local translational control mechanisms in neurons. Prerequisite for a functional role of the RNA in synaptodendritic domains is its targeted delivery along the dendritic extent. We report here that the targeting-competent 5' BC1 domain carries two dendritic targeting codes. One code, specifying somatic export, is located in the medial-basal region of the 5' BC1 stem-loop structure. It is defined by an export-determinant stem-bulge motif. The second code, specifying long-range dendritic delivery, is located in the apical part of the 5' stem-loop domain. This element features a GA kink-turn (KT) motif that is indispensable for distal targeting. It specifically interacts with heterogeneous nuclear ribonucleoprotein A2, a trans-acting targeting factor that has previously been implicated in the transport of MBP mRNA in oligodendrocytes and neurons. Our work suggests that a BC1 KT motif encodes distal targeting via the A2 pathway and that architectural RNA elements, such as KT motifs, may function as spatial codes in neural cells.

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Contributions of 5′ BC1 motifs to dendritic targeting competence. The histogram summarizes the quantitative analyses shown in Figs. 2–5. (blue) Wild type and mutants that are indistinguishable from wild type. (green) Mutants that lack the long-range distal dendritic targeting code, but retain the somatic export code. (yellow/red) Mutants that lack the somatic export code. See Fig. 1 for motif abbreviations. Quantitative data are presented as the mean ± the SEM of relative signal intensities along the dendritic extent.
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fig8: Contributions of 5′ BC1 motifs to dendritic targeting competence. The histogram summarizes the quantitative analyses shown in Figs. 2–5. (blue) Wild type and mutants that are indistinguishable from wild type. (green) Mutants that lack the long-range distal dendritic targeting code, but retain the somatic export code. (yellow/red) Mutants that lack the somatic export code. See Fig. 1 for motif abbreviations. Quantitative data are presented as the mean ± the SEM of relative signal intensities along the dendritic extent.

Mentions: 3D architectural motifs serve as determinants of RNA function (Noller, 2005). We surmised that in dendritic RNAs, such motifs, when contained within cis-acting DTEs, may be carriers of spatial codes. To test this hypothesis, we functionally dissected the targeting-determinant 5′ domain of dendritic BC1 RNA. Our data (see Fig. 8 for synopsis) show that at least two spatial codes are expressed in the 5′ BC1 domain; a code to specify somatic export, contained within a cis-acting element that we call BC1 DTE1, and a code to specify long-range distal dendritic targeting, contained within BC1 DTE2. We identify a stem-bulge motif in the medial-basal region of the 5′ BC1 domain as a proximal DTE1 determinant, whereas a KT motif in the apical region serves as a distal DTE2 determinant.


Spatial codes in dendritic BC1 RNA.

Muslimov IA, Iacoangeli A, Brosius J, Tiedge H - J. Cell Biol. (2006)

Contributions of 5′ BC1 motifs to dendritic targeting competence. The histogram summarizes the quantitative analyses shown in Figs. 2–5. (blue) Wild type and mutants that are indistinguishable from wild type. (green) Mutants that lack the long-range distal dendritic targeting code, but retain the somatic export code. (yellow/red) Mutants that lack the somatic export code. See Fig. 1 for motif abbreviations. Quantitative data are presented as the mean ± the SEM of relative signal intensities along the dendritic extent.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC1808587&req=5

fig8: Contributions of 5′ BC1 motifs to dendritic targeting competence. The histogram summarizes the quantitative analyses shown in Figs. 2–5. (blue) Wild type and mutants that are indistinguishable from wild type. (green) Mutants that lack the long-range distal dendritic targeting code, but retain the somatic export code. (yellow/red) Mutants that lack the somatic export code. See Fig. 1 for motif abbreviations. Quantitative data are presented as the mean ± the SEM of relative signal intensities along the dendritic extent.
Mentions: 3D architectural motifs serve as determinants of RNA function (Noller, 2005). We surmised that in dendritic RNAs, such motifs, when contained within cis-acting DTEs, may be carriers of spatial codes. To test this hypothesis, we functionally dissected the targeting-determinant 5′ domain of dendritic BC1 RNA. Our data (see Fig. 8 for synopsis) show that at least two spatial codes are expressed in the 5′ BC1 domain; a code to specify somatic export, contained within a cis-acting element that we call BC1 DTE1, and a code to specify long-range distal dendritic targeting, contained within BC1 DTE2. We identify a stem-bulge motif in the medial-basal region of the 5′ BC1 domain as a proximal DTE1 determinant, whereas a KT motif in the apical region serves as a distal DTE2 determinant.

Bottom Line: This element features a GA kink-turn (KT) motif that is indispensable for distal targeting.It specifically interacts with heterogeneous nuclear ribonucleoprotein A2, a trans-acting targeting factor that has previously been implicated in the transport of MBP mRNA in oligodendrocytes and neurons.Our work suggests that a BC1 KT motif encodes distal targeting via the A2 pathway and that architectural RNA elements, such as KT motifs, may function as spatial codes in neural cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology and Pharmacology, The Robert F. Furchgott Center for Neural and Behavioral Science, State University of New York Health Science Center at Brooklyn, Brooklyn, NY 11203, USA.

ABSTRACT
BC1 RNA is a dendritic untranslated RNA that has been implicated in local translational control mechanisms in neurons. Prerequisite for a functional role of the RNA in synaptodendritic domains is its targeted delivery along the dendritic extent. We report here that the targeting-competent 5' BC1 domain carries two dendritic targeting codes. One code, specifying somatic export, is located in the medial-basal region of the 5' BC1 stem-loop structure. It is defined by an export-determinant stem-bulge motif. The second code, specifying long-range dendritic delivery, is located in the apical part of the 5' stem-loop domain. This element features a GA kink-turn (KT) motif that is indispensable for distal targeting. It specifically interacts with heterogeneous nuclear ribonucleoprotein A2, a trans-acting targeting factor that has previously been implicated in the transport of MBP mRNA in oligodendrocytes and neurons. Our work suggests that a BC1 KT motif encodes distal targeting via the A2 pathway and that architectural RNA elements, such as KT motifs, may function as spatial codes in neural cells.

Show MeSH