Limits...
Primary histiocytic sarcoma arising in the head and neck with predominant spindle cell component.

Alexiev BA, Sailey CJ, McClure SA, Ord RA, Zhao XF, Papadimitriou JC - Diagn Pathol (2007)

Bottom Line: Sheets, fascicles, and whorls of spindle cells with spindled to ovoid vesicular nuclei, small to medium-sized distinct nucleoli, and eosinophilic cytoplasm were frequently observed.Focal expression of CD4 and S-100 was also noted.Chromosome study revealed a 57-80 hyperdiploid [7]/46, XY [13] karyotype, including 3 to 4 copies of various chromosomes.

View Article: PubMed Central - HTML - PubMed

Affiliation: University of Maryland Medical Center, Department of Pathology, NBW43, 22 S Greene Street, Baltimore, MD 21201, USA. balexiev@umm.edu

ABSTRACT
This is the first case report of Histiocytic Sarcoma (HS) with predominant spindle cell component occurring in the head and neck region of a 41-year-old man. The tumor was composed of sheets of large round to oval cells with pleomorphic vesicular nuclei, prominent nucleoli and abundant eosinophilic cytoplasm. Multinucleated forms, numerous mitoses, and tumor necrosis were also noted. Sheets, fascicles, and whorls of spindle cells with spindled to ovoid vesicular nuclei, small to medium-sized distinct nucleoli, and eosinophilic cytoplasm were frequently observed. Immunohistochemical staining in the tumor cells was positive for CD163, CD68, lysozyme, CD45, and NSE. Focal expression of CD4 and S-100 was also noted. Electron microscopy demonstrated an abundance of lysosomes in the cytoplasm of tumor cells. Chromosome study revealed a 57-80 hyperdiploid [7]/46, XY [13] karyotype, including 3 to 4 copies of various chromosomes. The immunohistochemical and ultrastructural findings confirmed the diagnosis of HS.

No MeSH data available.


Related in: MedlinePlus

Histiocytic sarcoma. Note prominent spindle cell differentiation. (hematoxylin-eosin, original magnification × 200).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC1808440&req=5

Figure 3: Histiocytic sarcoma. Note prominent spindle cell differentiation. (hematoxylin-eosin, original magnification × 200).

Mentions: Hematoxylin-eosin stained sections showed a tumor mass remotely resembling a completely effaced lymph node, composed of a diffuse proliferation of large round to oval cells with pleomorphic vesicular nuclei, prominent nucleoli and abundant eosinophilic cytoplasm (Fig. 2). Sheets, fascicles, and whorls of spindle cells with spindled to ovoid vesicular nuclei, small to medium, distinct nucleoli, and eosinophilic cytoplasm were frequently observed (Fig. 3). Large multinucleated cells, mitotic figures (27/10 HPFs), and necrosis were also noted. There were numerous admixed lymphocytes, polymorphonuclear leukocytes, and less commonly, plasma cells. The neoplastic cells invaded the lymph node capsule, perinodal soft tissues and periosteum. They were strongly positive for CD163 (Fig. 4), CD68, lysozyme, NSE, and vimentin. Weak to moderate immunostaining for S-100, CD4, and CD45 were also noted. Reactive lymphocytes stained strongly for CD3, CD4, CD8, and to a lesser degree for CD20. Residual CD21 positive, and CD23 positive follicular dentritic cells were also seen (Fig. 5). The Ki-67 index was approximately 70% (Fig. 6). The neoplastic cells were negative for CD43, ALK, CD30, EMA, smooth muscle actin, cytokeratin, desmin, myogenin, TTF1, CD99, CD1a, LMP-1, CD117, HMB45, and Mart-1/Melan A. Electron microscopy revealed presence of numerous lysosomes and lack of desmosomes, Birbeck granules and interdigitating cell processes (Fig. 7). In situ hybridization for EBV-encoded RNAs was negative. Cytogenetic studies revealed a 57–80 hyperdiploid [7]/46, XY [13] karyotype, including 3 to 4 copies of various chromosomes.


Primary histiocytic sarcoma arising in the head and neck with predominant spindle cell component.

Alexiev BA, Sailey CJ, McClure SA, Ord RA, Zhao XF, Papadimitriou JC - Diagn Pathol (2007)

Histiocytic sarcoma. Note prominent spindle cell differentiation. (hematoxylin-eosin, original magnification × 200).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC1808440&req=5

Figure 3: Histiocytic sarcoma. Note prominent spindle cell differentiation. (hematoxylin-eosin, original magnification × 200).
Mentions: Hematoxylin-eosin stained sections showed a tumor mass remotely resembling a completely effaced lymph node, composed of a diffuse proliferation of large round to oval cells with pleomorphic vesicular nuclei, prominent nucleoli and abundant eosinophilic cytoplasm (Fig. 2). Sheets, fascicles, and whorls of spindle cells with spindled to ovoid vesicular nuclei, small to medium, distinct nucleoli, and eosinophilic cytoplasm were frequently observed (Fig. 3). Large multinucleated cells, mitotic figures (27/10 HPFs), and necrosis were also noted. There were numerous admixed lymphocytes, polymorphonuclear leukocytes, and less commonly, plasma cells. The neoplastic cells invaded the lymph node capsule, perinodal soft tissues and periosteum. They were strongly positive for CD163 (Fig. 4), CD68, lysozyme, NSE, and vimentin. Weak to moderate immunostaining for S-100, CD4, and CD45 were also noted. Reactive lymphocytes stained strongly for CD3, CD4, CD8, and to a lesser degree for CD20. Residual CD21 positive, and CD23 positive follicular dentritic cells were also seen (Fig. 5). The Ki-67 index was approximately 70% (Fig. 6). The neoplastic cells were negative for CD43, ALK, CD30, EMA, smooth muscle actin, cytokeratin, desmin, myogenin, TTF1, CD99, CD1a, LMP-1, CD117, HMB45, and Mart-1/Melan A. Electron microscopy revealed presence of numerous lysosomes and lack of desmosomes, Birbeck granules and interdigitating cell processes (Fig. 7). In situ hybridization for EBV-encoded RNAs was negative. Cytogenetic studies revealed a 57–80 hyperdiploid [7]/46, XY [13] karyotype, including 3 to 4 copies of various chromosomes.

Bottom Line: Sheets, fascicles, and whorls of spindle cells with spindled to ovoid vesicular nuclei, small to medium-sized distinct nucleoli, and eosinophilic cytoplasm were frequently observed.Focal expression of CD4 and S-100 was also noted.Chromosome study revealed a 57-80 hyperdiploid [7]/46, XY [13] karyotype, including 3 to 4 copies of various chromosomes.

View Article: PubMed Central - HTML - PubMed

Affiliation: University of Maryland Medical Center, Department of Pathology, NBW43, 22 S Greene Street, Baltimore, MD 21201, USA. balexiev@umm.edu

ABSTRACT
This is the first case report of Histiocytic Sarcoma (HS) with predominant spindle cell component occurring in the head and neck region of a 41-year-old man. The tumor was composed of sheets of large round to oval cells with pleomorphic vesicular nuclei, prominent nucleoli and abundant eosinophilic cytoplasm. Multinucleated forms, numerous mitoses, and tumor necrosis were also noted. Sheets, fascicles, and whorls of spindle cells with spindled to ovoid vesicular nuclei, small to medium-sized distinct nucleoli, and eosinophilic cytoplasm were frequently observed. Immunohistochemical staining in the tumor cells was positive for CD163, CD68, lysozyme, CD45, and NSE. Focal expression of CD4 and S-100 was also noted. Electron microscopy demonstrated an abundance of lysosomes in the cytoplasm of tumor cells. Chromosome study revealed a 57-80 hyperdiploid [7]/46, XY [13] karyotype, including 3 to 4 copies of various chromosomes. The immunohistochemical and ultrastructural findings confirmed the diagnosis of HS.

No MeSH data available.


Related in: MedlinePlus