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Plasma levels of leptin, omentin, collagenous repeat-containing sequence of 26-kDa protein (CORS-26) and adiponectin before and after oral glucose uptake in slim adults.

Wurm S, Neumeier M, Weigert J, Schäffler A, Buechler C - Cardiovasc Diabetol (2007)

Bottom Line: Circulating leptin was reduced 2 h after glucose uptake whereas adiponectin and resistin levels are not changed.Omentin is highly abundant in plasma and immunoblot analysis revealed no alterations when plasma levels before and 2 h after glucose intake were compared.Taken together our data indicate that only leptin is reduced by glucose uptake in insulin-sensitive probands whereas adiponectin and resistin are not altered.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Internal Medicine I, Regensburg University Hospital, D-93042 Regensburg, Germany. sylvia_wurm@hotmail.com

ABSTRACT

Background: Adipose tissue secreted proteins are collectively named adipocytokines and include leptin, adiponectin, resistin, collagenous repeat-containing sequence of 26-kDa protein (CORS-26) and omentin. Several of these adipocytokines influence insulin sensitivity and glucose metabolism and therefore systemic levels may be affected by oral glucose uptake. Whereas contradictory results have been published for leptin and adiponectin, resistin has not been extensively investigated and no reports on omentin and CORS-26 do exist.

Methods: Therefore the plasma levels of these proteins before and 120 min after an oral glucose load were analyzed in 20 highly-insulin sensitive, young adults by ELISA or immunoblot.

Results: Circulating leptin was reduced 2 h after glucose uptake whereas adiponectin and resistin levels are not changed. Distribution of adiponectin and CORS-26 isoforms were similar before and after glucose ingestion. Omentin is highly abundant in plasma and immunoblot analysis revealed no alterations when plasma levels before and 2 h after glucose intake were compared.

Conclusion: Taken together our data indicate that only leptin is reduced by glucose uptake in insulin-sensitive probands whereas adiponectin and resistin are not altered. CORS-26 was demonstrated for the first time to circulate as high molecular weight form in plasma and like omentin was not influenced by oral glucose load. Omentin was shown to enhance insulin-stimulated glucose uptake but systemic levels are not correlated to postprandial blood glucose.

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Related in: MedlinePlus

Specificity of the omentin antiserum and omentin in fasting plasma and plasma after glucose challenge. (A) In vitro translated omentin and a control protein were used to determine the specificity of the omentin antiserum. (B) Omentin was analyzed in the plasma of all probands and a representative result of the probands 1 to 4 after an overnight fast (0) and 2 h (2) after glucose uptake by immunoblot is shown. (C) Omentin in the plasma of women (F9, F10, F12, F17) and men (M4, M8, M11, M13, M14, M15, M18, M19, M20) was determined by immunoblot.
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Figure 3: Specificity of the omentin antiserum and omentin in fasting plasma and plasma after glucose challenge. (A) In vitro translated omentin and a control protein were used to determine the specificity of the omentin antiserum. (B) Omentin was analyzed in the plasma of all probands and a representative result of the probands 1 to 4 after an overnight fast (0) and 2 h (2) after glucose uptake by immunoblot is shown. (C) Omentin in the plasma of women (F9, F10, F12, F17) and men (M4, M8, M11, M13, M14, M15, M18, M19, M20) was determined by immunoblot.

Mentions: In vitro translated omentin and a control protein with a C-terminal V5 tag were used for immunoblot using an anti-V5 antibody (not shown) or anti-omentin antibody (Figure 3A). Anti-omentin antibody specifically detects omentin protein and therefore was used for immunoblots. Plasma before and after glucose uptake was analyzed and omentin with a molecular weight of 30 kDa was found similar abundant and a representative result is shown in figure 3B. Serial dilution of plasma revealed that a difference of 20 % can be detected by immunoblot and the subsequent quantification of the signals with OptiQuant software (not shown).


Plasma levels of leptin, omentin, collagenous repeat-containing sequence of 26-kDa protein (CORS-26) and adiponectin before and after oral glucose uptake in slim adults.

Wurm S, Neumeier M, Weigert J, Schäffler A, Buechler C - Cardiovasc Diabetol (2007)

Specificity of the omentin antiserum and omentin in fasting plasma and plasma after glucose challenge. (A) In vitro translated omentin and a control protein were used to determine the specificity of the omentin antiserum. (B) Omentin was analyzed in the plasma of all probands and a representative result of the probands 1 to 4 after an overnight fast (0) and 2 h (2) after glucose uptake by immunoblot is shown. (C) Omentin in the plasma of women (F9, F10, F12, F17) and men (M4, M8, M11, M13, M14, M15, M18, M19, M20) was determined by immunoblot.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC1804262&req=5

Figure 3: Specificity of the omentin antiserum and omentin in fasting plasma and plasma after glucose challenge. (A) In vitro translated omentin and a control protein were used to determine the specificity of the omentin antiserum. (B) Omentin was analyzed in the plasma of all probands and a representative result of the probands 1 to 4 after an overnight fast (0) and 2 h (2) after glucose uptake by immunoblot is shown. (C) Omentin in the plasma of women (F9, F10, F12, F17) and men (M4, M8, M11, M13, M14, M15, M18, M19, M20) was determined by immunoblot.
Mentions: In vitro translated omentin and a control protein with a C-terminal V5 tag were used for immunoblot using an anti-V5 antibody (not shown) or anti-omentin antibody (Figure 3A). Anti-omentin antibody specifically detects omentin protein and therefore was used for immunoblots. Plasma before and after glucose uptake was analyzed and omentin with a molecular weight of 30 kDa was found similar abundant and a representative result is shown in figure 3B. Serial dilution of plasma revealed that a difference of 20 % can be detected by immunoblot and the subsequent quantification of the signals with OptiQuant software (not shown).

Bottom Line: Circulating leptin was reduced 2 h after glucose uptake whereas adiponectin and resistin levels are not changed.Omentin is highly abundant in plasma and immunoblot analysis revealed no alterations when plasma levels before and 2 h after glucose intake were compared.Taken together our data indicate that only leptin is reduced by glucose uptake in insulin-sensitive probands whereas adiponectin and resistin are not altered.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Internal Medicine I, Regensburg University Hospital, D-93042 Regensburg, Germany. sylvia_wurm@hotmail.com

ABSTRACT

Background: Adipose tissue secreted proteins are collectively named adipocytokines and include leptin, adiponectin, resistin, collagenous repeat-containing sequence of 26-kDa protein (CORS-26) and omentin. Several of these adipocytokines influence insulin sensitivity and glucose metabolism and therefore systemic levels may be affected by oral glucose uptake. Whereas contradictory results have been published for leptin and adiponectin, resistin has not been extensively investigated and no reports on omentin and CORS-26 do exist.

Methods: Therefore the plasma levels of these proteins before and 120 min after an oral glucose load were analyzed in 20 highly-insulin sensitive, young adults by ELISA or immunoblot.

Results: Circulating leptin was reduced 2 h after glucose uptake whereas adiponectin and resistin levels are not changed. Distribution of adiponectin and CORS-26 isoforms were similar before and after glucose ingestion. Omentin is highly abundant in plasma and immunoblot analysis revealed no alterations when plasma levels before and 2 h after glucose intake were compared.

Conclusion: Taken together our data indicate that only leptin is reduced by glucose uptake in insulin-sensitive probands whereas adiponectin and resistin are not altered. CORS-26 was demonstrated for the first time to circulate as high molecular weight form in plasma and like omentin was not influenced by oral glucose load. Omentin was shown to enhance insulin-stimulated glucose uptake but systemic levels are not correlated to postprandial blood glucose.

Show MeSH
Related in: MedlinePlus