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Plasma levels of leptin, omentin, collagenous repeat-containing sequence of 26-kDa protein (CORS-26) and adiponectin before and after oral glucose uptake in slim adults.

Wurm S, Neumeier M, Weigert J, Schäffler A, Buechler C - Cardiovasc Diabetol (2007)

Bottom Line: Circulating leptin was reduced 2 h after glucose uptake whereas adiponectin and resistin levels are not changed.Omentin is highly abundant in plasma and immunoblot analysis revealed no alterations when plasma levels before and 2 h after glucose intake were compared.Taken together our data indicate that only leptin is reduced by glucose uptake in insulin-sensitive probands whereas adiponectin and resistin are not altered.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Internal Medicine I, Regensburg University Hospital, D-93042 Regensburg, Germany. sylvia_wurm@hotmail.com

ABSTRACT

Background: Adipose tissue secreted proteins are collectively named adipocytokines and include leptin, adiponectin, resistin, collagenous repeat-containing sequence of 26-kDa protein (CORS-26) and omentin. Several of these adipocytokines influence insulin sensitivity and glucose metabolism and therefore systemic levels may be affected by oral glucose uptake. Whereas contradictory results have been published for leptin and adiponectin, resistin has not been extensively investigated and no reports on omentin and CORS-26 do exist.

Methods: Therefore the plasma levels of these proteins before and 120 min after an oral glucose load were analyzed in 20 highly-insulin sensitive, young adults by ELISA or immunoblot.

Results: Circulating leptin was reduced 2 h after glucose uptake whereas adiponectin and resistin levels are not changed. Distribution of adiponectin and CORS-26 isoforms were similar before and after glucose ingestion. Omentin is highly abundant in plasma and immunoblot analysis revealed no alterations when plasma levels before and 2 h after glucose intake were compared.

Conclusion: Taken together our data indicate that only leptin is reduced by glucose uptake in insulin-sensitive probands whereas adiponectin and resistin are not altered. CORS-26 was demonstrated for the first time to circulate as high molecular weight form in plasma and like omentin was not influenced by oral glucose load. Omentin was shown to enhance insulin-stimulated glucose uptake but systemic levels are not correlated to postprandial blood glucose.

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Specificity of the CORS-26 antiserum and CORS-26 in fasting plasma and plasma after glucose challenge. (A) Recombinant adiponectin (Apm) and CORS-26 on a silver-stained SDS-PAGE gel and upon detection by the respective antibodies using immunoblot. (B) High-molecular weight (HMW) CORS-26 was analyzed by non-denaturing SDS-PAGE and low-molecular weight (LMW) CORS-26 by denaturing SDS-PAGE and a representative result of 4 donors (1 to 4) after fasting (0) and 2 h (2) after glucose uptake is shown. The molecular mass of standard proteins in kDa is indicated on the left side of the immunoblot.
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Figure 2: Specificity of the CORS-26 antiserum and CORS-26 in fasting plasma and plasma after glucose challenge. (A) Recombinant adiponectin (Apm) and CORS-26 on a silver-stained SDS-PAGE gel and upon detection by the respective antibodies using immunoblot. (B) High-molecular weight (HMW) CORS-26 was analyzed by non-denaturing SDS-PAGE and low-molecular weight (LMW) CORS-26 by denaturing SDS-PAGE and a representative result of 4 donors (1 to 4) after fasting (0) and 2 h (2) after glucose uptake is shown. The molecular mass of standard proteins in kDa is indicated on the left side of the immunoblot.

Mentions: Specificity of the CORS-26 antibody was analyzed by immunoblot using recombinant adiponectin and recombinant CORS-26 produced in insect cells [7,12]. 100 ng of protein was separated by SDS-PAGE and silver staining of the gel revealed similar amounts of protein (Figure 2A). The membranes were hybridized with adiponectin or CORS-26 antibody and each antiserum revealed a specific signal with the respective protein (Figure 2A).


Plasma levels of leptin, omentin, collagenous repeat-containing sequence of 26-kDa protein (CORS-26) and adiponectin before and after oral glucose uptake in slim adults.

Wurm S, Neumeier M, Weigert J, Schäffler A, Buechler C - Cardiovasc Diabetol (2007)

Specificity of the CORS-26 antiserum and CORS-26 in fasting plasma and plasma after glucose challenge. (A) Recombinant adiponectin (Apm) and CORS-26 on a silver-stained SDS-PAGE gel and upon detection by the respective antibodies using immunoblot. (B) High-molecular weight (HMW) CORS-26 was analyzed by non-denaturing SDS-PAGE and low-molecular weight (LMW) CORS-26 by denaturing SDS-PAGE and a representative result of 4 donors (1 to 4) after fasting (0) and 2 h (2) after glucose uptake is shown. The molecular mass of standard proteins in kDa is indicated on the left side of the immunoblot.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC1804262&req=5

Figure 2: Specificity of the CORS-26 antiserum and CORS-26 in fasting plasma and plasma after glucose challenge. (A) Recombinant adiponectin (Apm) and CORS-26 on a silver-stained SDS-PAGE gel and upon detection by the respective antibodies using immunoblot. (B) High-molecular weight (HMW) CORS-26 was analyzed by non-denaturing SDS-PAGE and low-molecular weight (LMW) CORS-26 by denaturing SDS-PAGE and a representative result of 4 donors (1 to 4) after fasting (0) and 2 h (2) after glucose uptake is shown. The molecular mass of standard proteins in kDa is indicated on the left side of the immunoblot.
Mentions: Specificity of the CORS-26 antibody was analyzed by immunoblot using recombinant adiponectin and recombinant CORS-26 produced in insect cells [7,12]. 100 ng of protein was separated by SDS-PAGE and silver staining of the gel revealed similar amounts of protein (Figure 2A). The membranes were hybridized with adiponectin or CORS-26 antibody and each antiserum revealed a specific signal with the respective protein (Figure 2A).

Bottom Line: Circulating leptin was reduced 2 h after glucose uptake whereas adiponectin and resistin levels are not changed.Omentin is highly abundant in plasma and immunoblot analysis revealed no alterations when plasma levels before and 2 h after glucose intake were compared.Taken together our data indicate that only leptin is reduced by glucose uptake in insulin-sensitive probands whereas adiponectin and resistin are not altered.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Internal Medicine I, Regensburg University Hospital, D-93042 Regensburg, Germany. sylvia_wurm@hotmail.com

ABSTRACT

Background: Adipose tissue secreted proteins are collectively named adipocytokines and include leptin, adiponectin, resistin, collagenous repeat-containing sequence of 26-kDa protein (CORS-26) and omentin. Several of these adipocytokines influence insulin sensitivity and glucose metabolism and therefore systemic levels may be affected by oral glucose uptake. Whereas contradictory results have been published for leptin and adiponectin, resistin has not been extensively investigated and no reports on omentin and CORS-26 do exist.

Methods: Therefore the plasma levels of these proteins before and 120 min after an oral glucose load were analyzed in 20 highly-insulin sensitive, young adults by ELISA or immunoblot.

Results: Circulating leptin was reduced 2 h after glucose uptake whereas adiponectin and resistin levels are not changed. Distribution of adiponectin and CORS-26 isoforms were similar before and after glucose ingestion. Omentin is highly abundant in plasma and immunoblot analysis revealed no alterations when plasma levels before and 2 h after glucose intake were compared.

Conclusion: Taken together our data indicate that only leptin is reduced by glucose uptake in insulin-sensitive probands whereas adiponectin and resistin are not altered. CORS-26 was demonstrated for the first time to circulate as high molecular weight form in plasma and like omentin was not influenced by oral glucose load. Omentin was shown to enhance insulin-stimulated glucose uptake but systemic levels are not correlated to postprandial blood glucose.

Show MeSH
Related in: MedlinePlus