Limits...
Phenotypic characteristics of human monocytes undergoing transendothelial migration.

Grisar J, Hahn P, Brosch S, Peterlik M, Smolen JS, Pietschmann P - Arthritis Res. (2001)

Bottom Line: We found that monocyte migration led to an enhanced expression of CD11a, CD33, CD45RO, CD54 [intercellular cell-adhesion molecule (ICAM)-1] and human leucocyte antigen-DR.Taken together, the results suggest that local cytokine production activating ECs is sufficient to enhance monocyte migration and that this, in turn, can induce changes consistent with an activated phenotype known to be interactive between antigen-presenting cells and T cells.These results have implications for our pathogenetic insights into rheumatoid arthritis.

View Article: PubMed Central - PubMed

Affiliation: Division of Rheumatology, Department of Internal Medicine III, University of Vienna, Währinger Gürtel 18-20, A-1180 Vienna, Austria. johannes.grisar@akh-wien.ac.at

ABSTRACT
In our study we characterised the immunophenotype of monocytes that migrated through an endothelial cell (EC) monolayer in vitro. We found that monocyte migration led to an enhanced expression of CD11a, CD33, CD45RO, CD54 [intercellular cell-adhesion molecule (ICAM)-1] and human leucocyte antigen-DR. The most striking increase was observed for ICAM-1 when ECs were activated with tumour necrosis factor-alpha and interleukin-1alpha. The results of our study indicate the following: (1) there is a characteristic immunophenotype on the surface of monocytes after transendothelial migration; (2) this phenotype seems to be induced by interactions between monocytes and ECs; and (3) this change is enhanced by the pretreatment of ECs with cytokines. Taken together, the results suggest that local cytokine production activating ECs is sufficient to enhance monocyte migration and that this, in turn, can induce changes consistent with an activated phenotype known to be interactive between antigen-presenting cells and T cells. These results have implications for our pathogenetic insights into rheumatoid arthritis.

Show MeSH

Related in: MedlinePlus

Endothelium enhances monocyte migration. The percentages of peripheral blood mononuclear cells (PBMCs) migrated in the absence (white columns) or presence (black columns) of endothelial cells are shown at different time points. Results are means ± SD for at least three independent experiments. Asterisks denote significant (P < 0.05) differences between the percentages of cells migrated in the absence of endothelium and in its presence.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC17829&req=5

Figure 1: Endothelium enhances monocyte migration. The percentages of peripheral blood mononuclear cells (PBMCs) migrated in the absence (white columns) or presence (black columns) of endothelial cells are shown at different time points. Results are means ± SD for at least three independent experiments. Asterisks denote significant (P < 0.05) differences between the percentages of cells migrated in the absence of endothelium and in its presence.

Mentions: In initial experiments we studied the time course of PBMC migration into plain or EC-coated collagen gels, respectively. As shown in Fig. 1, the presence of an endothelium clearly facilitated the migration of PBMCs: after 30 minutes the percentage of PBMCs that had migrated was twice as high as that in the absence of ECs. After 2 hours, about 40% of the PBMCs could be recovered from collagen gels coated with an EC layer, whereas only 24% of PBMCs had migrated into plain collagen gels. Prolonging the incubation time to 24 hours allowed further migration of PBMCs only in the absence of ECs, but did not significantly increase the extent of EC-mediated migration.


Phenotypic characteristics of human monocytes undergoing transendothelial migration.

Grisar J, Hahn P, Brosch S, Peterlik M, Smolen JS, Pietschmann P - Arthritis Res. (2001)

Endothelium enhances monocyte migration. The percentages of peripheral blood mononuclear cells (PBMCs) migrated in the absence (white columns) or presence (black columns) of endothelial cells are shown at different time points. Results are means ± SD for at least three independent experiments. Asterisks denote significant (P < 0.05) differences between the percentages of cells migrated in the absence of endothelium and in its presence.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC17829&req=5

Figure 1: Endothelium enhances monocyte migration. The percentages of peripheral blood mononuclear cells (PBMCs) migrated in the absence (white columns) or presence (black columns) of endothelial cells are shown at different time points. Results are means ± SD for at least three independent experiments. Asterisks denote significant (P < 0.05) differences between the percentages of cells migrated in the absence of endothelium and in its presence.
Mentions: In initial experiments we studied the time course of PBMC migration into plain or EC-coated collagen gels, respectively. As shown in Fig. 1, the presence of an endothelium clearly facilitated the migration of PBMCs: after 30 minutes the percentage of PBMCs that had migrated was twice as high as that in the absence of ECs. After 2 hours, about 40% of the PBMCs could be recovered from collagen gels coated with an EC layer, whereas only 24% of PBMCs had migrated into plain collagen gels. Prolonging the incubation time to 24 hours allowed further migration of PBMCs only in the absence of ECs, but did not significantly increase the extent of EC-mediated migration.

Bottom Line: We found that monocyte migration led to an enhanced expression of CD11a, CD33, CD45RO, CD54 [intercellular cell-adhesion molecule (ICAM)-1] and human leucocyte antigen-DR.Taken together, the results suggest that local cytokine production activating ECs is sufficient to enhance monocyte migration and that this, in turn, can induce changes consistent with an activated phenotype known to be interactive between antigen-presenting cells and T cells.These results have implications for our pathogenetic insights into rheumatoid arthritis.

View Article: PubMed Central - PubMed

Affiliation: Division of Rheumatology, Department of Internal Medicine III, University of Vienna, Währinger Gürtel 18-20, A-1180 Vienna, Austria. johannes.grisar@akh-wien.ac.at

ABSTRACT
In our study we characterised the immunophenotype of monocytes that migrated through an endothelial cell (EC) monolayer in vitro. We found that monocyte migration led to an enhanced expression of CD11a, CD33, CD45RO, CD54 [intercellular cell-adhesion molecule (ICAM)-1] and human leucocyte antigen-DR. The most striking increase was observed for ICAM-1 when ECs were activated with tumour necrosis factor-alpha and interleukin-1alpha. The results of our study indicate the following: (1) there is a characteristic immunophenotype on the surface of monocytes after transendothelial migration; (2) this phenotype seems to be induced by interactions between monocytes and ECs; and (3) this change is enhanced by the pretreatment of ECs with cytokines. Taken together, the results suggest that local cytokine production activating ECs is sufficient to enhance monocyte migration and that this, in turn, can induce changes consistent with an activated phenotype known to be interactive between antigen-presenting cells and T cells. These results have implications for our pathogenetic insights into rheumatoid arthritis.

Show MeSH
Related in: MedlinePlus