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Synovial stromal cells from rheumatoid arthritis patients attract monocytes by producing MCP-1 and IL-8.

Hayashida K, Nanki T, Girschick H, Yavuz S, Ochi T, Lipsky PE - Arthritis Res. (2001)

Bottom Line: Macrophages that accumulate in the synovium of rheumatoid arthritis patients play an important role in the pathogenesis of this inflammatory disease.However, the mechanism by which macrophages are attracted into the inflamed synovium and accumulate there has not been completely delineated.These results suggest that one of the mechanisms by which macrophages accumulate in the inflamed synovium is by responding to the chemokines produced locally.

View Article: PubMed Central - PubMed

Affiliation: University of Texas, Southwestern Medical Center, Dallas, Texas, USA. lipskyp@mail.nih.gov

ABSTRACT
Macrophages that accumulate in the synovium of rheumatoid arthritis patients play an important role in the pathogenesis of this inflammatory disease. However, the mechanism by which macrophages are attracted into the inflamed synovium and accumulate there has not been completely delineated. The results of this study show that rheumatoid arthritis synovial stromal cells produce the chemokines monocyte chemotactic protein-1 and IL-8, and these have the capacity to attract peripheral monocytes. These results suggest that one of the mechanisms by which macrophages accumulate in the inflamed synovium is by responding to the chemokines produced locally.

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Monocyte migration induced by supernatants of RA SCLs stimulated with TNF-α. Assay of monocyte migration was performed using supernatants of RA SCLs stimulated with TNF-α. Each RA SCL (1 × 105/well) was cultured in six-well culture plates with 2 ml DMEM supplemented with 5% FBS with or without 2 ng/ml TNF-α. Supernatants were collected after 24 h of incubation, and were used for assay. Migrated cells were stained with anti-CD14 mAb, then the number of migrated CD14+ cells was assessed by flow cytometry. RA6/1, RA8/3, and Sy77 are RA SCLs. The mean and SEM were calculated from three independent experiments. Statistical analysis was performed with the paired Student t test. *P < 0.05 and **P < 0.01.
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Figure 7: Monocyte migration induced by supernatants of RA SCLs stimulated with TNF-α. Assay of monocyte migration was performed using supernatants of RA SCLs stimulated with TNF-α. Each RA SCL (1 × 105/well) was cultured in six-well culture plates with 2 ml DMEM supplemented with 5% FBS with or without 2 ng/ml TNF-α. Supernatants were collected after 24 h of incubation, and were used for assay. Migrated cells were stained with anti-CD14 mAb, then the number of migrated CD14+ cells was assessed by flow cytometry. RA6/1, RA8/3, and Sy77 are RA SCLs. The mean and SEM were calculated from three independent experiments. Statistical analysis was performed with the paired Student t test. *P < 0.05 and **P < 0.01.

Mentions: RA SCLs were stimulated with TNF-α, and chemokine production and the capacity of supernatants to influence monocyte migration were examined to assess the influence of inflammatory cytokines on chemokine production and monocyte trafficking. Supernatants from RA SCLs stimulated with 2 ng/ml TNF-α produced more MCP-1, IL-8, RANTES and IP-10 (Fig. 6), and attracted more monocytes (Fig. 7) than those that were unstimulated.


Synovial stromal cells from rheumatoid arthritis patients attract monocytes by producing MCP-1 and IL-8.

Hayashida K, Nanki T, Girschick H, Yavuz S, Ochi T, Lipsky PE - Arthritis Res. (2001)

Monocyte migration induced by supernatants of RA SCLs stimulated with TNF-α. Assay of monocyte migration was performed using supernatants of RA SCLs stimulated with TNF-α. Each RA SCL (1 × 105/well) was cultured in six-well culture plates with 2 ml DMEM supplemented with 5% FBS with or without 2 ng/ml TNF-α. Supernatants were collected after 24 h of incubation, and were used for assay. Migrated cells were stained with anti-CD14 mAb, then the number of migrated CD14+ cells was assessed by flow cytometry. RA6/1, RA8/3, and Sy77 are RA SCLs. The mean and SEM were calculated from three independent experiments. Statistical analysis was performed with the paired Student t test. *P < 0.05 and **P < 0.01.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC17828&req=5

Figure 7: Monocyte migration induced by supernatants of RA SCLs stimulated with TNF-α. Assay of monocyte migration was performed using supernatants of RA SCLs stimulated with TNF-α. Each RA SCL (1 × 105/well) was cultured in six-well culture plates with 2 ml DMEM supplemented with 5% FBS with or without 2 ng/ml TNF-α. Supernatants were collected after 24 h of incubation, and were used for assay. Migrated cells were stained with anti-CD14 mAb, then the number of migrated CD14+ cells was assessed by flow cytometry. RA6/1, RA8/3, and Sy77 are RA SCLs. The mean and SEM were calculated from three independent experiments. Statistical analysis was performed with the paired Student t test. *P < 0.05 and **P < 0.01.
Mentions: RA SCLs were stimulated with TNF-α, and chemokine production and the capacity of supernatants to influence monocyte migration were examined to assess the influence of inflammatory cytokines on chemokine production and monocyte trafficking. Supernatants from RA SCLs stimulated with 2 ng/ml TNF-α produced more MCP-1, IL-8, RANTES and IP-10 (Fig. 6), and attracted more monocytes (Fig. 7) than those that were unstimulated.

Bottom Line: Macrophages that accumulate in the synovium of rheumatoid arthritis patients play an important role in the pathogenesis of this inflammatory disease.However, the mechanism by which macrophages are attracted into the inflamed synovium and accumulate there has not been completely delineated.These results suggest that one of the mechanisms by which macrophages accumulate in the inflamed synovium is by responding to the chemokines produced locally.

View Article: PubMed Central - PubMed

Affiliation: University of Texas, Southwestern Medical Center, Dallas, Texas, USA. lipskyp@mail.nih.gov

ABSTRACT
Macrophages that accumulate in the synovium of rheumatoid arthritis patients play an important role in the pathogenesis of this inflammatory disease. However, the mechanism by which macrophages are attracted into the inflamed synovium and accumulate there has not been completely delineated. The results of this study show that rheumatoid arthritis synovial stromal cells produce the chemokines monocyte chemotactic protein-1 and IL-8, and these have the capacity to attract peripheral monocytes. These results suggest that one of the mechanisms by which macrophages accumulate in the inflamed synovium is by responding to the chemokines produced locally.

Show MeSH
Related in: MedlinePlus