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Synovial stromal cells from rheumatoid arthritis patients attract monocytes by producing MCP-1 and IL-8.

Hayashida K, Nanki T, Girschick H, Yavuz S, Ochi T, Lipsky PE - Arthritis Res. (2001)

Bottom Line: Macrophages that accumulate in the synovium of rheumatoid arthritis patients play an important role in the pathogenesis of this inflammatory disease.However, the mechanism by which macrophages are attracted into the inflamed synovium and accumulate there has not been completely delineated.These results suggest that one of the mechanisms by which macrophages accumulate in the inflamed synovium is by responding to the chemokines produced locally.

View Article: PubMed Central - PubMed

Affiliation: University of Texas, Southwestern Medical Center, Dallas, Texas, USA. lipskyp@mail.nih.gov

ABSTRACT
Macrophages that accumulate in the synovium of rheumatoid arthritis patients play an important role in the pathogenesis of this inflammatory disease. However, the mechanism by which macrophages are attracted into the inflamed synovium and accumulate there has not been completely delineated. The results of this study show that rheumatoid arthritis synovial stromal cells produce the chemokines monocyte chemotactic protein-1 and IL-8, and these have the capacity to attract peripheral monocytes. These results suggest that one of the mechanisms by which macrophages accumulate in the inflamed synovium is by responding to the chemokines produced locally.

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Chemokine production by RA SCL stimulated with TNF-α. Production of MCP-1, IL-8, RANTES, IP-10, and MIP-1β was measured by ELISA. Various cell lines (1 × 105/ml) were cultured for 24 h in 2 ml DMEM containing 5% FBS with or without 2 ng/ml TNF-α, and supernatants were collected. The content of (A) MCP-1, (B) IL-8, (C) RANTES and (D) IP-10 are shown; MIP-1β was not detected (less than 10 pg/ml). RA6/1, RA8/3, and Sy77 are RA SCLs. The mean and SEM were calculated from triplicates of one representative experiment out of three with similar results. Statistical analysis was performed with the Student t test. *P < 0.05 and **P < 0.01.
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Figure 6: Chemokine production by RA SCL stimulated with TNF-α. Production of MCP-1, IL-8, RANTES, IP-10, and MIP-1β was measured by ELISA. Various cell lines (1 × 105/ml) were cultured for 24 h in 2 ml DMEM containing 5% FBS with or without 2 ng/ml TNF-α, and supernatants were collected. The content of (A) MCP-1, (B) IL-8, (C) RANTES and (D) IP-10 are shown; MIP-1β was not detected (less than 10 pg/ml). RA6/1, RA8/3, and Sy77 are RA SCLs. The mean and SEM were calculated from triplicates of one representative experiment out of three with similar results. Statistical analysis was performed with the Student t test. *P < 0.05 and **P < 0.01.

Mentions: RA SCLs were stimulated with TNF-α, and chemokine production and the capacity of supernatants to influence monocyte migration were examined to assess the influence of inflammatory cytokines on chemokine production and monocyte trafficking. Supernatants from RA SCLs stimulated with 2 ng/ml TNF-α produced more MCP-1, IL-8, RANTES and IP-10 (Fig. 6), and attracted more monocytes (Fig. 7) than those that were unstimulated.


Synovial stromal cells from rheumatoid arthritis patients attract monocytes by producing MCP-1 and IL-8.

Hayashida K, Nanki T, Girschick H, Yavuz S, Ochi T, Lipsky PE - Arthritis Res. (2001)

Chemokine production by RA SCL stimulated with TNF-α. Production of MCP-1, IL-8, RANTES, IP-10, and MIP-1β was measured by ELISA. Various cell lines (1 × 105/ml) were cultured for 24 h in 2 ml DMEM containing 5% FBS with or without 2 ng/ml TNF-α, and supernatants were collected. The content of (A) MCP-1, (B) IL-8, (C) RANTES and (D) IP-10 are shown; MIP-1β was not detected (less than 10 pg/ml). RA6/1, RA8/3, and Sy77 are RA SCLs. The mean and SEM were calculated from triplicates of one representative experiment out of three with similar results. Statistical analysis was performed with the Student t test. *P < 0.05 and **P < 0.01.
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Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC17828&req=5

Figure 6: Chemokine production by RA SCL stimulated with TNF-α. Production of MCP-1, IL-8, RANTES, IP-10, and MIP-1β was measured by ELISA. Various cell lines (1 × 105/ml) were cultured for 24 h in 2 ml DMEM containing 5% FBS with or without 2 ng/ml TNF-α, and supernatants were collected. The content of (A) MCP-1, (B) IL-8, (C) RANTES and (D) IP-10 are shown; MIP-1β was not detected (less than 10 pg/ml). RA6/1, RA8/3, and Sy77 are RA SCLs. The mean and SEM were calculated from triplicates of one representative experiment out of three with similar results. Statistical analysis was performed with the Student t test. *P < 0.05 and **P < 0.01.
Mentions: RA SCLs were stimulated with TNF-α, and chemokine production and the capacity of supernatants to influence monocyte migration were examined to assess the influence of inflammatory cytokines on chemokine production and monocyte trafficking. Supernatants from RA SCLs stimulated with 2 ng/ml TNF-α produced more MCP-1, IL-8, RANTES and IP-10 (Fig. 6), and attracted more monocytes (Fig. 7) than those that were unstimulated.

Bottom Line: Macrophages that accumulate in the synovium of rheumatoid arthritis patients play an important role in the pathogenesis of this inflammatory disease.However, the mechanism by which macrophages are attracted into the inflamed synovium and accumulate there has not been completely delineated.These results suggest that one of the mechanisms by which macrophages accumulate in the inflamed synovium is by responding to the chemokines produced locally.

View Article: PubMed Central - PubMed

Affiliation: University of Texas, Southwestern Medical Center, Dallas, Texas, USA. lipskyp@mail.nih.gov

ABSTRACT
Macrophages that accumulate in the synovium of rheumatoid arthritis patients play an important role in the pathogenesis of this inflammatory disease. However, the mechanism by which macrophages are attracted into the inflamed synovium and accumulate there has not been completely delineated. The results of this study show that rheumatoid arthritis synovial stromal cells produce the chemokines monocyte chemotactic protein-1 and IL-8, and these have the capacity to attract peripheral monocytes. These results suggest that one of the mechanisms by which macrophages accumulate in the inflamed synovium is by responding to the chemokines produced locally.

Show MeSH
Related in: MedlinePlus