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Synovial stromal cells from rheumatoid arthritis patients attract monocytes by producing MCP-1 and IL-8.

Hayashida K, Nanki T, Girschick H, Yavuz S, Ochi T, Lipsky PE - Arthritis Res. (2001)

Bottom Line: Macrophages that accumulate in the synovium of rheumatoid arthritis patients play an important role in the pathogenesis of this inflammatory disease.However, the mechanism by which macrophages are attracted into the inflamed synovium and accumulate there has not been completely delineated.These results suggest that one of the mechanisms by which macrophages accumulate in the inflamed synovium is by responding to the chemokines produced locally.

View Article: PubMed Central - PubMed

Affiliation: University of Texas, Southwestern Medical Center, Dallas, Texas, USA. lipskyp@mail.nih.gov

ABSTRACT
Macrophages that accumulate in the synovium of rheumatoid arthritis patients play an important role in the pathogenesis of this inflammatory disease. However, the mechanism by which macrophages are attracted into the inflamed synovium and accumulate there has not been completely delineated. The results of this study show that rheumatoid arthritis synovial stromal cells produce the chemokines monocyte chemotactic protein-1 and IL-8, and these have the capacity to attract peripheral monocytes. These results suggest that one of the mechanisms by which macrophages accumulate in the inflamed synovium is by responding to the chemokines produced locally.

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Chemokine mRNA expression by various cell lines. Chemokine mRNA expression was examined with RT-PCR. PCR products were separated in 1.5% agarose gels and analyzed after ethidium bromide staining. Each PCR was performed using 0.1 μl cDNA sample and the number of PCR cycles indicated. RA6/1, RA8/3, and Sy77 are RA SCLs, OA5/26 is a SCL from OA, and FBHG is a skin fibroblast line.
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Figure 3: Chemokine mRNA expression by various cell lines. Chemokine mRNA expression was examined with RT-PCR. PCR products were separated in 1.5% agarose gels and analyzed after ethidium bromide staining. Each PCR was performed using 0.1 μl cDNA sample and the number of PCR cycles indicated. RA6/1, RA8/3, and Sy77 are RA SCLs, OA5/26 is a SCL from OA, and FBHG is a skin fibroblast line.

Mentions: Production of proinflammatory chemokine mRNA by the various cell lines was assessed using RT-PCR (Fig. 3). RA SCLs expressed a number of proinflammatory chemokine mRNAs, including MCP-1, IL-8, MIP-1α, MIP-1β, RANTES, and IP-10. OA SCL also expressed these same chemokine mRNAs with the exception of MIP-1β. The fibroblast line expressed mRNAs for IL-8, MCP-1, and IP-10, but not RANTES, MIP-1α, and MIP-1β.


Synovial stromal cells from rheumatoid arthritis patients attract monocytes by producing MCP-1 and IL-8.

Hayashida K, Nanki T, Girschick H, Yavuz S, Ochi T, Lipsky PE - Arthritis Res. (2001)

Chemokine mRNA expression by various cell lines. Chemokine mRNA expression was examined with RT-PCR. PCR products were separated in 1.5% agarose gels and analyzed after ethidium bromide staining. Each PCR was performed using 0.1 μl cDNA sample and the number of PCR cycles indicated. RA6/1, RA8/3, and Sy77 are RA SCLs, OA5/26 is a SCL from OA, and FBHG is a skin fibroblast line.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC17828&req=5

Figure 3: Chemokine mRNA expression by various cell lines. Chemokine mRNA expression was examined with RT-PCR. PCR products were separated in 1.5% agarose gels and analyzed after ethidium bromide staining. Each PCR was performed using 0.1 μl cDNA sample and the number of PCR cycles indicated. RA6/1, RA8/3, and Sy77 are RA SCLs, OA5/26 is a SCL from OA, and FBHG is a skin fibroblast line.
Mentions: Production of proinflammatory chemokine mRNA by the various cell lines was assessed using RT-PCR (Fig. 3). RA SCLs expressed a number of proinflammatory chemokine mRNAs, including MCP-1, IL-8, MIP-1α, MIP-1β, RANTES, and IP-10. OA SCL also expressed these same chemokine mRNAs with the exception of MIP-1β. The fibroblast line expressed mRNAs for IL-8, MCP-1, and IP-10, but not RANTES, MIP-1α, and MIP-1β.

Bottom Line: Macrophages that accumulate in the synovium of rheumatoid arthritis patients play an important role in the pathogenesis of this inflammatory disease.However, the mechanism by which macrophages are attracted into the inflamed synovium and accumulate there has not been completely delineated.These results suggest that one of the mechanisms by which macrophages accumulate in the inflamed synovium is by responding to the chemokines produced locally.

View Article: PubMed Central - PubMed

Affiliation: University of Texas, Southwestern Medical Center, Dallas, Texas, USA. lipskyp@mail.nih.gov

ABSTRACT
Macrophages that accumulate in the synovium of rheumatoid arthritis patients play an important role in the pathogenesis of this inflammatory disease. However, the mechanism by which macrophages are attracted into the inflamed synovium and accumulate there has not been completely delineated. The results of this study show that rheumatoid arthritis synovial stromal cells produce the chemokines monocyte chemotactic protein-1 and IL-8, and these have the capacity to attract peripheral monocytes. These results suggest that one of the mechanisms by which macrophages accumulate in the inflamed synovium is by responding to the chemokines produced locally.

Show MeSH
Related in: MedlinePlus