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Synovial stromal cells from rheumatoid arthritis patients attract monocytes by producing MCP-1 and IL-8.

Hayashida K, Nanki T, Girschick H, Yavuz S, Ochi T, Lipsky PE - Arthritis Res. (2001)

Bottom Line: Macrophages that accumulate in the synovium of rheumatoid arthritis patients play an important role in the pathogenesis of this inflammatory disease.However, the mechanism by which macrophages are attracted into the inflamed synovium and accumulate there has not been completely delineated.These results suggest that one of the mechanisms by which macrophages accumulate in the inflamed synovium is by responding to the chemokines produced locally.

View Article: PubMed Central - PubMed

Affiliation: University of Texas, Southwestern Medical Center, Dallas, Texas, USA. lipskyp@mail.nih.gov

ABSTRACT
Macrophages that accumulate in the synovium of rheumatoid arthritis patients play an important role in the pathogenesis of this inflammatory disease. However, the mechanism by which macrophages are attracted into the inflamed synovium and accumulate there has not been completely delineated. The results of this study show that rheumatoid arthritis synovial stromal cells produce the chemokines monocyte chemotactic protein-1 and IL-8, and these have the capacity to attract peripheral monocytes. These results suggest that one of the mechanisms by which macrophages accumulate in the inflamed synovium is by responding to the chemokines produced locally.

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Related in: MedlinePlus

Monocyte migration induced by supernatants of various cell lines. An assay of monocyte migration was performed using supernatants of various cells. Each cell line (1 × 105/well) was cultured in six-well culture plates with 2 ml DMEM supplemented with 5% FBS. Supernatants were collected after 24 h of incubation, and were used for assay. Migrated cells were stained with anti-CD14 mAb, then the number of migrated CD14+ cells was assessed by flow cytometry. RA6/1, RA8/3, and Sy77 are RA SCLs, OA5/26 is a SCL from OA, and FBHG is a skin fibroblast line. The mean and SEM were calculated with data from 11 independent experiments. Statistical analysis was performed with the paired Student t test. *P < 0.05 and **P < 0.01.
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Figure 2: Monocyte migration induced by supernatants of various cell lines. An assay of monocyte migration was performed using supernatants of various cells. Each cell line (1 × 105/well) was cultured in six-well culture plates with 2 ml DMEM supplemented with 5% FBS. Supernatants were collected after 24 h of incubation, and were used for assay. Migrated cells were stained with anti-CD14 mAb, then the number of migrated CD14+ cells was assessed by flow cytometry. RA6/1, RA8/3, and Sy77 are RA SCLs, OA5/26 is a SCL from OA, and FBHG is a skin fibroblast line. The mean and SEM were calculated with data from 11 independent experiments. Statistical analysis was performed with the paired Student t test. *P < 0.05 and **P < 0.01.

Mentions: To examine whether SCLs derived from RA synovium can attract monocytes efficiently, migration of monocytes in response to culture supernatants of RA and OA SCLs and fibroblasts was examined (Fig. 2). Supernatants of all cell lines attracted significantly more monocytes than medium alone (P < 0.01). Those supernatants from RA SCLs induced the migration of significantly more monocytes than the supernatants of the OA SCL and skin fibroblasts.


Synovial stromal cells from rheumatoid arthritis patients attract monocytes by producing MCP-1 and IL-8.

Hayashida K, Nanki T, Girschick H, Yavuz S, Ochi T, Lipsky PE - Arthritis Res. (2001)

Monocyte migration induced by supernatants of various cell lines. An assay of monocyte migration was performed using supernatants of various cells. Each cell line (1 × 105/well) was cultured in six-well culture plates with 2 ml DMEM supplemented with 5% FBS. Supernatants were collected after 24 h of incubation, and were used for assay. Migrated cells were stained with anti-CD14 mAb, then the number of migrated CD14+ cells was assessed by flow cytometry. RA6/1, RA8/3, and Sy77 are RA SCLs, OA5/26 is a SCL from OA, and FBHG is a skin fibroblast line. The mean and SEM were calculated with data from 11 independent experiments. Statistical analysis was performed with the paired Student t test. *P < 0.05 and **P < 0.01.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC17828&req=5

Figure 2: Monocyte migration induced by supernatants of various cell lines. An assay of monocyte migration was performed using supernatants of various cells. Each cell line (1 × 105/well) was cultured in six-well culture plates with 2 ml DMEM supplemented with 5% FBS. Supernatants were collected after 24 h of incubation, and were used for assay. Migrated cells were stained with anti-CD14 mAb, then the number of migrated CD14+ cells was assessed by flow cytometry. RA6/1, RA8/3, and Sy77 are RA SCLs, OA5/26 is a SCL from OA, and FBHG is a skin fibroblast line. The mean and SEM were calculated with data from 11 independent experiments. Statistical analysis was performed with the paired Student t test. *P < 0.05 and **P < 0.01.
Mentions: To examine whether SCLs derived from RA synovium can attract monocytes efficiently, migration of monocytes in response to culture supernatants of RA and OA SCLs and fibroblasts was examined (Fig. 2). Supernatants of all cell lines attracted significantly more monocytes than medium alone (P < 0.01). Those supernatants from RA SCLs induced the migration of significantly more monocytes than the supernatants of the OA SCL and skin fibroblasts.

Bottom Line: Macrophages that accumulate in the synovium of rheumatoid arthritis patients play an important role in the pathogenesis of this inflammatory disease.However, the mechanism by which macrophages are attracted into the inflamed synovium and accumulate there has not been completely delineated.These results suggest that one of the mechanisms by which macrophages accumulate in the inflamed synovium is by responding to the chemokines produced locally.

View Article: PubMed Central - PubMed

Affiliation: University of Texas, Southwestern Medical Center, Dallas, Texas, USA. lipskyp@mail.nih.gov

ABSTRACT
Macrophages that accumulate in the synovium of rheumatoid arthritis patients play an important role in the pathogenesis of this inflammatory disease. However, the mechanism by which macrophages are attracted into the inflamed synovium and accumulate there has not been completely delineated. The results of this study show that rheumatoid arthritis synovial stromal cells produce the chemokines monocyte chemotactic protein-1 and IL-8, and these have the capacity to attract peripheral monocytes. These results suggest that one of the mechanisms by which macrophages accumulate in the inflamed synovium is by responding to the chemokines produced locally.

Show MeSH
Related in: MedlinePlus