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Synovial stromal cells from rheumatoid arthritis patients attract monocytes by producing MCP-1 and IL-8.

Hayashida K, Nanki T, Girschick H, Yavuz S, Ochi T, Lipsky PE - Arthritis Res. (2001)

Bottom Line: Macrophages that accumulate in the synovium of rheumatoid arthritis patients play an important role in the pathogenesis of this inflammatory disease.However, the mechanism by which macrophages are attracted into the inflamed synovium and accumulate there has not been completely delineated.These results suggest that one of the mechanisms by which macrophages accumulate in the inflamed synovium is by responding to the chemokines produced locally.

View Article: PubMed Central - PubMed

Affiliation: University of Texas, Southwestern Medical Center, Dallas, Texas, USA. lipskyp@mail.nih.gov

ABSTRACT
Macrophages that accumulate in the synovium of rheumatoid arthritis patients play an important role in the pathogenesis of this inflammatory disease. However, the mechanism by which macrophages are attracted into the inflamed synovium and accumulate there has not been completely delineated. The results of this study show that rheumatoid arthritis synovial stromal cells produce the chemokines monocyte chemotactic protein-1 and IL-8, and these have the capacity to attract peripheral monocytes. These results suggest that one of the mechanisms by which macrophages accumulate in the inflamed synovium is by responding to the chemokines produced locally.

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Chemokine receptor expression by peripheral blood monocytes. Peripheral blood SRBC rosette negative cells (1 × 105) were stained with anti-CD14 mAb and various chemokine receptor mAbs, and were analyzed by flow cytometry. CD14+ cells were gated, and chemokine receptor expression by the CD14+ monocytes is shown (solid line). Dotted lines show staining by isotype-matched control mAb. Percentage of positive cells is also shown in the histograms. Staining was from one of nine experiments. FL-1 height, FITC fluorescence; FL-2 height, PE fluorescence.
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Figure 1: Chemokine receptor expression by peripheral blood monocytes. Peripheral blood SRBC rosette negative cells (1 × 105) were stained with anti-CD14 mAb and various chemokine receptor mAbs, and were analyzed by flow cytometry. CD14+ cells were gated, and chemokine receptor expression by the CD14+ monocytes is shown (solid line). Dotted lines show staining by isotype-matched control mAb. Percentage of positive cells is also shown in the histograms. Staining was from one of nine experiments. FL-1 height, FITC fluorescence; FL-2 height, PE fluorescence.

Mentions: Chemokine receptor expression by CD14+ monocytes was examined using flow cytometry. Representative examples (Fig. 1) and a summary of staining results (Table 1) are presented. CXCR3 and CXCR4 were expressed by a high frequency of monocytes in all healthy donors. More than one-half of monocytes expressed CCR2, CCR5, CXCR1, and CXCR2 in seven of nine cases, although a minimal number of monocytes from two donors expressed these receptors. Expression of CCR6 and CXCR5 by monocytes was minimal in all subjects. CCR1 was expressed by few monocytes in three donors, whereas 20-40% of monocytes of the other donors expressed CCR1. These results suggest that CCR1, CCR2, CCR5, and CXCR1, CXCR2, CXCR3, and CXCR4 are candidates to be involved in chemokine-mediated trafficking of monocytes.


Synovial stromal cells from rheumatoid arthritis patients attract monocytes by producing MCP-1 and IL-8.

Hayashida K, Nanki T, Girschick H, Yavuz S, Ochi T, Lipsky PE - Arthritis Res. (2001)

Chemokine receptor expression by peripheral blood monocytes. Peripheral blood SRBC rosette negative cells (1 × 105) were stained with anti-CD14 mAb and various chemokine receptor mAbs, and were analyzed by flow cytometry. CD14+ cells were gated, and chemokine receptor expression by the CD14+ monocytes is shown (solid line). Dotted lines show staining by isotype-matched control mAb. Percentage of positive cells is also shown in the histograms. Staining was from one of nine experiments. FL-1 height, FITC fluorescence; FL-2 height, PE fluorescence.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC17828&req=5

Figure 1: Chemokine receptor expression by peripheral blood monocytes. Peripheral blood SRBC rosette negative cells (1 × 105) were stained with anti-CD14 mAb and various chemokine receptor mAbs, and were analyzed by flow cytometry. CD14+ cells were gated, and chemokine receptor expression by the CD14+ monocytes is shown (solid line). Dotted lines show staining by isotype-matched control mAb. Percentage of positive cells is also shown in the histograms. Staining was from one of nine experiments. FL-1 height, FITC fluorescence; FL-2 height, PE fluorescence.
Mentions: Chemokine receptor expression by CD14+ monocytes was examined using flow cytometry. Representative examples (Fig. 1) and a summary of staining results (Table 1) are presented. CXCR3 and CXCR4 were expressed by a high frequency of monocytes in all healthy donors. More than one-half of monocytes expressed CCR2, CCR5, CXCR1, and CXCR2 in seven of nine cases, although a minimal number of monocytes from two donors expressed these receptors. Expression of CCR6 and CXCR5 by monocytes was minimal in all subjects. CCR1 was expressed by few monocytes in three donors, whereas 20-40% of monocytes of the other donors expressed CCR1. These results suggest that CCR1, CCR2, CCR5, and CXCR1, CXCR2, CXCR3, and CXCR4 are candidates to be involved in chemokine-mediated trafficking of monocytes.

Bottom Line: Macrophages that accumulate in the synovium of rheumatoid arthritis patients play an important role in the pathogenesis of this inflammatory disease.However, the mechanism by which macrophages are attracted into the inflamed synovium and accumulate there has not been completely delineated.These results suggest that one of the mechanisms by which macrophages accumulate in the inflamed synovium is by responding to the chemokines produced locally.

View Article: PubMed Central - PubMed

Affiliation: University of Texas, Southwestern Medical Center, Dallas, Texas, USA. lipskyp@mail.nih.gov

ABSTRACT
Macrophages that accumulate in the synovium of rheumatoid arthritis patients play an important role in the pathogenesis of this inflammatory disease. However, the mechanism by which macrophages are attracted into the inflamed synovium and accumulate there has not been completely delineated. The results of this study show that rheumatoid arthritis synovial stromal cells produce the chemokines monocyte chemotactic protein-1 and IL-8, and these have the capacity to attract peripheral monocytes. These results suggest that one of the mechanisms by which macrophages accumulate in the inflamed synovium is by responding to the chemokines produced locally.

Show MeSH
Related in: MedlinePlus