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The development of clinical signs of rheumatoid synovial inflammation is associated with increased synthesis of the chemokine CXCL8 (interleukin-8).

Kraan MC, Patel DD, Haringman JJ, Smith MD, Weedon H, Ahern MJ, Breedveld FC, Tak PP - Arthritis Res. (2000)

Bottom Line: Paired synovial tissue samples were obtained from both clinically uninvolved (CU) and clinically involved (CI) knee joints of eight rheumatoid arthritis (RA) patients.We observed the expression of the chemokines CXCL8, CXCL9, CXCL10, CCL2 and CCL4 in CI and CU joints of RA patients.In particular, CXCL8 protein levels were specifically increased in CI joints compared with CU joints, which was confirmed by immunohistochemistry and in situ hybridization.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Clinical Immunology and Rheumatology, Department of Medicine, Academic Medical Center, Amsterdam, The Netherlands. m.c.kraan@amc.uva.nl

ABSTRACT
Paired synovial tissue samples were obtained from both clinically uninvolved (CU) and clinically involved (CI) knee joints of eight rheumatoid arthritis (RA) patients. In addition, biopsies were taken from five control subjects. We observed the expression of the chemokines CXCL8, CXCL9, CXCL10, CCL2 and CCL4 in CI and CU joints of RA patients. In particular, CXCL8 protein levels were specifically increased in CI joints compared with CU joints, which was confirmed by immunohistochemistry and in situ hybridization.

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Interleukin-8 (CXCL8) expression and mRNA levels in synovial tissue (ST) from clinically uninvolved (CU) and from clinically involved (CI) joints in paired synovial biopsies from seven patients with rheumatoid arthritis (RA) (connected, filled circles). Also shown are results from one unpaired (open circles) synovial biopsy. CXCL8 levels are expressed as integrated optical density measured with digital image analysis on ST sections stained immunohistochemically and with in situ hybridization.
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Figure 4: Interleukin-8 (CXCL8) expression and mRNA levels in synovial tissue (ST) from clinically uninvolved (CU) and from clinically involved (CI) joints in paired synovial biopsies from seven patients with rheumatoid arthritis (RA) (connected, filled circles). Also shown are results from one unpaired (open circles) synovial biopsy. CXCL8 levels are expressed as integrated optical density measured with digital image analysis on ST sections stained immunohistochemically and with in situ hybridization.

Mentions: To confirm and extend the observation that CXCL8 expression was higher in CI joints than in CU joints, immunohistologic analysis was performed. In one of the patients, the ST from the CI joint was not assessable. Expression of CXCL8 was observed in both the intimal lining layer and the synovial sublining (Fig. 2). There was markedly increased CXCL8 protein expression in six of the seven CI joints (IOD 65,961 ± 5364) compared with CU joints (IOD 28,552 ± 5749) (P < 0.05), whereas the eight CU joints showed similar results compared with the five control subjects (IOD 34,599 ± 5144) (Figs 3 and 4).


The development of clinical signs of rheumatoid synovial inflammation is associated with increased synthesis of the chemokine CXCL8 (interleukin-8).

Kraan MC, Patel DD, Haringman JJ, Smith MD, Weedon H, Ahern MJ, Breedveld FC, Tak PP - Arthritis Res. (2000)

Interleukin-8 (CXCL8) expression and mRNA levels in synovial tissue (ST) from clinically uninvolved (CU) and from clinically involved (CI) joints in paired synovial biopsies from seven patients with rheumatoid arthritis (RA) (connected, filled circles). Also shown are results from one unpaired (open circles) synovial biopsy. CXCL8 levels are expressed as integrated optical density measured with digital image analysis on ST sections stained immunohistochemically and with in situ hybridization.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC17826&req=5

Figure 4: Interleukin-8 (CXCL8) expression and mRNA levels in synovial tissue (ST) from clinically uninvolved (CU) and from clinically involved (CI) joints in paired synovial biopsies from seven patients with rheumatoid arthritis (RA) (connected, filled circles). Also shown are results from one unpaired (open circles) synovial biopsy. CXCL8 levels are expressed as integrated optical density measured with digital image analysis on ST sections stained immunohistochemically and with in situ hybridization.
Mentions: To confirm and extend the observation that CXCL8 expression was higher in CI joints than in CU joints, immunohistologic analysis was performed. In one of the patients, the ST from the CI joint was not assessable. Expression of CXCL8 was observed in both the intimal lining layer and the synovial sublining (Fig. 2). There was markedly increased CXCL8 protein expression in six of the seven CI joints (IOD 65,961 ± 5364) compared with CU joints (IOD 28,552 ± 5749) (P < 0.05), whereas the eight CU joints showed similar results compared with the five control subjects (IOD 34,599 ± 5144) (Figs 3 and 4).

Bottom Line: Paired synovial tissue samples were obtained from both clinically uninvolved (CU) and clinically involved (CI) knee joints of eight rheumatoid arthritis (RA) patients.We observed the expression of the chemokines CXCL8, CXCL9, CXCL10, CCL2 and CCL4 in CI and CU joints of RA patients.In particular, CXCL8 protein levels were specifically increased in CI joints compared with CU joints, which was confirmed by immunohistochemistry and in situ hybridization.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Clinical Immunology and Rheumatology, Department of Medicine, Academic Medical Center, Amsterdam, The Netherlands. m.c.kraan@amc.uva.nl

ABSTRACT
Paired synovial tissue samples were obtained from both clinically uninvolved (CU) and clinically involved (CI) knee joints of eight rheumatoid arthritis (RA) patients. In addition, biopsies were taken from five control subjects. We observed the expression of the chemokines CXCL8, CXCL9, CXCL10, CCL2 and CCL4 in CI and CU joints of RA patients. In particular, CXCL8 protein levels were specifically increased in CI joints compared with CU joints, which was confirmed by immunohistochemistry and in situ hybridization.

Show MeSH
Related in: MedlinePlus