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The development of clinical signs of rheumatoid synovial inflammation is associated with increased synthesis of the chemokine CXCL8 (interleukin-8).

Kraan MC, Patel DD, Haringman JJ, Smith MD, Weedon H, Ahern MJ, Breedveld FC, Tak PP - Arthritis Res. (2000)

Bottom Line: Paired synovial tissue samples were obtained from both clinically uninvolved (CU) and clinically involved (CI) knee joints of eight rheumatoid arthritis (RA) patients.We observed the expression of the chemokines CXCL8, CXCL9, CXCL10, CCL2 and CCL4 in CI and CU joints of RA patients.In particular, CXCL8 protein levels were specifically increased in CI joints compared with CU joints, which was confirmed by immunohistochemistry and in situ hybridization.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Clinical Immunology and Rheumatology, Department of Medicine, Academic Medical Center, Amsterdam, The Netherlands. m.c.kraan@amc.uva.nl

ABSTRACT
Paired synovial tissue samples were obtained from both clinically uninvolved (CU) and clinically involved (CI) knee joints of eight rheumatoid arthritis (RA) patients. In addition, biopsies were taken from five control subjects. We observed the expression of the chemokines CXCL8, CXCL9, CXCL10, CCL2 and CCL4 in CI and CU joints of RA patients. In particular, CXCL8 protein levels were specifically increased in CI joints compared with CU joints, which was confirmed by immunohistochemistry and in situ hybridization.

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Representative examples of synovial tissue sections stained for interleukin-8 (CXCL8) protein expression [immunohistochemistry, CXCL8 monoclonal antibody (mAb)] and CXCL8 mRNA expression (in-situ hybridization, anti-sense CXCL8 riboprobe) in normal synovium and in synovial tissue from paired clinically uninvolved knee joints and clinically involved knee joints of rheumatoid arthritis (RA) patients and control sections with a sense probe or aspecific mAb.
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Figure 2: Representative examples of synovial tissue sections stained for interleukin-8 (CXCL8) protein expression [immunohistochemistry, CXCL8 monoclonal antibody (mAb)] and CXCL8 mRNA expression (in-situ hybridization, anti-sense CXCL8 riboprobe) in normal synovium and in synovial tissue from paired clinically uninvolved knee joints and clinically involved knee joints of rheumatoid arthritis (RA) patients and control sections with a sense probe or aspecific mAb.

Mentions: To confirm and extend the observation that CXCL8 expression was higher in CI joints than in CU joints, immunohistologic analysis was performed. In one of the patients, the ST from the CI joint was not assessable. Expression of CXCL8 was observed in both the intimal lining layer and the synovial sublining (Fig. 2). There was markedly increased CXCL8 protein expression in six of the seven CI joints (IOD 65,961 ± 5364) compared with CU joints (IOD 28,552 ± 5749) (P < 0.05), whereas the eight CU joints showed similar results compared with the five control subjects (IOD 34,599 ± 5144) (Figs 3 and 4).


The development of clinical signs of rheumatoid synovial inflammation is associated with increased synthesis of the chemokine CXCL8 (interleukin-8).

Kraan MC, Patel DD, Haringman JJ, Smith MD, Weedon H, Ahern MJ, Breedveld FC, Tak PP - Arthritis Res. (2000)

Representative examples of synovial tissue sections stained for interleukin-8 (CXCL8) protein expression [immunohistochemistry, CXCL8 monoclonal antibody (mAb)] and CXCL8 mRNA expression (in-situ hybridization, anti-sense CXCL8 riboprobe) in normal synovium and in synovial tissue from paired clinically uninvolved knee joints and clinically involved knee joints of rheumatoid arthritis (RA) patients and control sections with a sense probe or aspecific mAb.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC17826&req=5

Figure 2: Representative examples of synovial tissue sections stained for interleukin-8 (CXCL8) protein expression [immunohistochemistry, CXCL8 monoclonal antibody (mAb)] and CXCL8 mRNA expression (in-situ hybridization, anti-sense CXCL8 riboprobe) in normal synovium and in synovial tissue from paired clinically uninvolved knee joints and clinically involved knee joints of rheumatoid arthritis (RA) patients and control sections with a sense probe or aspecific mAb.
Mentions: To confirm and extend the observation that CXCL8 expression was higher in CI joints than in CU joints, immunohistologic analysis was performed. In one of the patients, the ST from the CI joint was not assessable. Expression of CXCL8 was observed in both the intimal lining layer and the synovial sublining (Fig. 2). There was markedly increased CXCL8 protein expression in six of the seven CI joints (IOD 65,961 ± 5364) compared with CU joints (IOD 28,552 ± 5749) (P < 0.05), whereas the eight CU joints showed similar results compared with the five control subjects (IOD 34,599 ± 5144) (Figs 3 and 4).

Bottom Line: Paired synovial tissue samples were obtained from both clinically uninvolved (CU) and clinically involved (CI) knee joints of eight rheumatoid arthritis (RA) patients.We observed the expression of the chemokines CXCL8, CXCL9, CXCL10, CCL2 and CCL4 in CI and CU joints of RA patients.In particular, CXCL8 protein levels were specifically increased in CI joints compared with CU joints, which was confirmed by immunohistochemistry and in situ hybridization.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Clinical Immunology and Rheumatology, Department of Medicine, Academic Medical Center, Amsterdam, The Netherlands. m.c.kraan@amc.uva.nl

ABSTRACT
Paired synovial tissue samples were obtained from both clinically uninvolved (CU) and clinically involved (CI) knee joints of eight rheumatoid arthritis (RA) patients. In addition, biopsies were taken from five control subjects. We observed the expression of the chemokines CXCL8, CXCL9, CXCL10, CCL2 and CCL4 in CI and CU joints of RA patients. In particular, CXCL8 protein levels were specifically increased in CI joints compared with CU joints, which was confirmed by immunohistochemistry and in situ hybridization.

Show MeSH
Related in: MedlinePlus