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The development of clinical signs of rheumatoid synovial inflammation is associated with increased synthesis of the chemokine CXCL8 (interleukin-8).

Kraan MC, Patel DD, Haringman JJ, Smith MD, Weedon H, Ahern MJ, Breedveld FC, Tak PP - Arthritis Res. (2000)

Bottom Line: Paired synovial tissue samples were obtained from both clinically uninvolved (CU) and clinically involved (CI) knee joints of eight rheumatoid arthritis (RA) patients.We observed the expression of the chemokines CXCL8, CXCL9, CXCL10, CCL2 and CCL4 in CI and CU joints of RA patients.In particular, CXCL8 protein levels were specifically increased in CI joints compared with CU joints, which was confirmed by immunohistochemistry and in situ hybridization.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Clinical Immunology and Rheumatology, Department of Medicine, Academic Medical Center, Amsterdam, The Netherlands. m.c.kraan@amc.uva.nl

ABSTRACT
Paired synovial tissue samples were obtained from both clinically uninvolved (CU) and clinically involved (CI) knee joints of eight rheumatoid arthritis (RA) patients. In addition, biopsies were taken from five control subjects. We observed the expression of the chemokines CXCL8, CXCL9, CXCL10, CCL2 and CCL4 in CI and CU joints of RA patients. In particular, CXCL8 protein levels were specifically increased in CI joints compared with CU joints, which was confirmed by immunohistochemistry and in situ hybridization.

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Chemokine levels in tissue extracts from clinically uninvolved (CU) compared to clinically involved (CI) joints in paired (connected, filled circles) synovial biopsies from eight patients with RA. Also shown are results from unpaired (open circles) synovial biopsies. Chemokine levels are expressed as ng/mg of tissue extract.
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Figure 1: Chemokine levels in tissue extracts from clinically uninvolved (CU) compared to clinically involved (CI) joints in paired (connected, filled circles) synovial biopsies from eight patients with RA. Also shown are results from unpaired (open circles) synovial biopsies. Chemokine levels are expressed as ng/mg of tissue extract.

Mentions: Differences in chemokine expression between control, CI, and CU joints were screened by determining protein expression in synovial extracts. The CXC chemokines CXCL8, CXCL9, and CXCL10, and the CC chemokines CCL2 and CCL4 could be detected in rheumatoid ST. In three of the eight patients, CXCL8 expression was detected in the CU joint (0.1 ± 0.06 ng of chemokine/mg of tissue extract; mean ± SEM) and it was increased in the CI joint (0.3 ± 0.13 ng/mg), but CXCL8 was not detectable in the two control patients that were tested (Fig. 1). CCL2 expression was detected in five of six CU joints (0.9 ± 0.5 ng/mg), two of four patients showed increased expression in the CI joint (1.0 ± 0.2 ng/mg), and one of two controls had detectable levels (0.1 ± 0.0 ng/mg) (Fig. 1). CXCL9 expression was detected in four of the six CU joints (9.1 ± 8.3 ng/mg), two of four patients showed increased expression in CI joints (8.0 ± 2.7 ng/mg), and CXCL9 was not detectable in two controls (Fig. 1). CXCL10 expression was detected in two of the six CU joints (0.2 ± 0.2 ng/mg), whereas two of four patients showed increased expression in CI joints (0.4 ± 0.2 ng/mg), and CXCL10 was not detectable in two controls (Fig. 1). CCL4 expression was detected in three of six CU joints (0.1 ± 0.1 ng/mg); one of four patients showed increased expression in the CI joint (0.2 ± 0.1 ng/mg), and CCL4 was not detectable in two controls (Fig. 1). Differences between CI knees, CU knees and control patients were not statistically significant, possibly owing to the small numbers of patients.


The development of clinical signs of rheumatoid synovial inflammation is associated with increased synthesis of the chemokine CXCL8 (interleukin-8).

Kraan MC, Patel DD, Haringman JJ, Smith MD, Weedon H, Ahern MJ, Breedveld FC, Tak PP - Arthritis Res. (2000)

Chemokine levels in tissue extracts from clinically uninvolved (CU) compared to clinically involved (CI) joints in paired (connected, filled circles) synovial biopsies from eight patients with RA. Also shown are results from unpaired (open circles) synovial biopsies. Chemokine levels are expressed as ng/mg of tissue extract.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC17826&req=5

Figure 1: Chemokine levels in tissue extracts from clinically uninvolved (CU) compared to clinically involved (CI) joints in paired (connected, filled circles) synovial biopsies from eight patients with RA. Also shown are results from unpaired (open circles) synovial biopsies. Chemokine levels are expressed as ng/mg of tissue extract.
Mentions: Differences in chemokine expression between control, CI, and CU joints were screened by determining protein expression in synovial extracts. The CXC chemokines CXCL8, CXCL9, and CXCL10, and the CC chemokines CCL2 and CCL4 could be detected in rheumatoid ST. In three of the eight patients, CXCL8 expression was detected in the CU joint (0.1 ± 0.06 ng of chemokine/mg of tissue extract; mean ± SEM) and it was increased in the CI joint (0.3 ± 0.13 ng/mg), but CXCL8 was not detectable in the two control patients that were tested (Fig. 1). CCL2 expression was detected in five of six CU joints (0.9 ± 0.5 ng/mg), two of four patients showed increased expression in the CI joint (1.0 ± 0.2 ng/mg), and one of two controls had detectable levels (0.1 ± 0.0 ng/mg) (Fig. 1). CXCL9 expression was detected in four of the six CU joints (9.1 ± 8.3 ng/mg), two of four patients showed increased expression in CI joints (8.0 ± 2.7 ng/mg), and CXCL9 was not detectable in two controls (Fig. 1). CXCL10 expression was detected in two of the six CU joints (0.2 ± 0.2 ng/mg), whereas two of four patients showed increased expression in CI joints (0.4 ± 0.2 ng/mg), and CXCL10 was not detectable in two controls (Fig. 1). CCL4 expression was detected in three of six CU joints (0.1 ± 0.1 ng/mg); one of four patients showed increased expression in the CI joint (0.2 ± 0.1 ng/mg), and CCL4 was not detectable in two controls (Fig. 1). Differences between CI knees, CU knees and control patients were not statistically significant, possibly owing to the small numbers of patients.

Bottom Line: Paired synovial tissue samples were obtained from both clinically uninvolved (CU) and clinically involved (CI) knee joints of eight rheumatoid arthritis (RA) patients.We observed the expression of the chemokines CXCL8, CXCL9, CXCL10, CCL2 and CCL4 in CI and CU joints of RA patients.In particular, CXCL8 protein levels were specifically increased in CI joints compared with CU joints, which was confirmed by immunohistochemistry and in situ hybridization.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Clinical Immunology and Rheumatology, Department of Medicine, Academic Medical Center, Amsterdam, The Netherlands. m.c.kraan@amc.uva.nl

ABSTRACT
Paired synovial tissue samples were obtained from both clinically uninvolved (CU) and clinically involved (CI) knee joints of eight rheumatoid arthritis (RA) patients. In addition, biopsies were taken from five control subjects. We observed the expression of the chemokines CXCL8, CXCL9, CXCL10, CCL2 and CCL4 in CI and CU joints of RA patients. In particular, CXCL8 protein levels were specifically increased in CI joints compared with CU joints, which was confirmed by immunohistochemistry and in situ hybridization.

Show MeSH
Related in: MedlinePlus