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Synovial cytokine mRNA expression during arthritis triggered by CpG motifs of bacterial DNA.

Deng GM, Tarkowski A - Arthritis Res. (2000)

Bottom Line: Our results show that cytokines derived from macrophages play an important role in pathogenesis of arthritis triggered by CpG oligodinucleotide (CpG ODN).IL-12 is in this respect an important immunomodulator during the development of joint inflammation.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Rheumatology, University of Göteborg, Göteborg, Sweden. guo-min@immuno.gu.se

ABSTRACT
Our results show that cytokines derived from macrophages play an important role in pathogenesis of arthritis triggered by CpG oligodinucleotide (CpG ODN). IL-12 is in this respect an important immunomodulator during the development of joint inflammation.

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Related in: MedlinePlus

TNF-α production in vitro by spleen mononuclear cells from IL-12 knockout mice (IL-12–/–) in comparison with cells from their wild-type littermates (IL-12+/+), after stimulation with CpG ODN (1 μm) or lipopolysaccharides (LPS) (1 μg/ml). Values are means and SEM from three mice per group. *P < 0.05. NS = not significant.
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Figure 5: TNF-α production in vitro by spleen mononuclear cells from IL-12 knockout mice (IL-12–/–) in comparison with cells from their wild-type littermates (IL-12+/+), after stimulation with CpG ODN (1 μm) or lipopolysaccharides (LPS) (1 μg/ml). Values are means and SEM from three mice per group. *P < 0.05. NS = not significant.

Mentions: To assess why the incidence and severity of CpG ODN-triggered arthritis were decreased in IL-12 knockout mice, we compared TNF-α levels in IL-12 knockout mice with those in wild-type mice, using CpG ODN as stimulus in vitro or in vivo. The reason for this approach is that TNF-α is an essential mediator of CpG ODN-mediated arthritis [10]. TNF-α levels in supernatants from mononuclear cells stimulated with CpG ODN were lower in IL-12 knockout mice than in the wild-type control mice (Fig. 5). In contrast, the TNF-α levels were similar in supernatants from mononuclear cells stimulated with lipopolysaccharides, irrespective of IL-12 phenotype (Fig. 5). This suggests that the TNF-α response by macrophages to CpG ODN stimulation is at least partly influenced by expression of IL-12. Serum TNF-α levels collected at day 3 after intra-articular inoculation with 6 μg CpG ODN from wild-type and IL-12 knockout mice did not show detectable amounts of this cytokine.


Synovial cytokine mRNA expression during arthritis triggered by CpG motifs of bacterial DNA.

Deng GM, Tarkowski A - Arthritis Res. (2000)

TNF-α production in vitro by spleen mononuclear cells from IL-12 knockout mice (IL-12–/–) in comparison with cells from their wild-type littermates (IL-12+/+), after stimulation with CpG ODN (1 μm) or lipopolysaccharides (LPS) (1 μg/ml). Values are means and SEM from three mice per group. *P < 0.05. NS = not significant.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC17824&req=5

Figure 5: TNF-α production in vitro by spleen mononuclear cells from IL-12 knockout mice (IL-12–/–) in comparison with cells from their wild-type littermates (IL-12+/+), after stimulation with CpG ODN (1 μm) or lipopolysaccharides (LPS) (1 μg/ml). Values are means and SEM from three mice per group. *P < 0.05. NS = not significant.
Mentions: To assess why the incidence and severity of CpG ODN-triggered arthritis were decreased in IL-12 knockout mice, we compared TNF-α levels in IL-12 knockout mice with those in wild-type mice, using CpG ODN as stimulus in vitro or in vivo. The reason for this approach is that TNF-α is an essential mediator of CpG ODN-mediated arthritis [10]. TNF-α levels in supernatants from mononuclear cells stimulated with CpG ODN were lower in IL-12 knockout mice than in the wild-type control mice (Fig. 5). In contrast, the TNF-α levels were similar in supernatants from mononuclear cells stimulated with lipopolysaccharides, irrespective of IL-12 phenotype (Fig. 5). This suggests that the TNF-α response by macrophages to CpG ODN stimulation is at least partly influenced by expression of IL-12. Serum TNF-α levels collected at day 3 after intra-articular inoculation with 6 μg CpG ODN from wild-type and IL-12 knockout mice did not show detectable amounts of this cytokine.

Bottom Line: Our results show that cytokines derived from macrophages play an important role in pathogenesis of arthritis triggered by CpG oligodinucleotide (CpG ODN).IL-12 is in this respect an important immunomodulator during the development of joint inflammation.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Rheumatology, University of Göteborg, Göteborg, Sweden. guo-min@immuno.gu.se

ABSTRACT
Our results show that cytokines derived from macrophages play an important role in pathogenesis of arthritis triggered by CpG oligodinucleotide (CpG ODN). IL-12 is in this respect an important immunomodulator during the development of joint inflammation.

Show MeSH
Related in: MedlinePlus