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Synovial cytokine mRNA expression during arthritis triggered by CpG motifs of bacterial DNA.

Deng GM, Tarkowski A - Arthritis Res. (2000)

Bottom Line: Our results show that cytokines derived from macrophages play an important role in pathogenesis of arthritis triggered by CpG oligodinucleotide (CpG ODN).IL-12 is in this respect an important immunomodulator during the development of joint inflammation.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Rheumatology, University of Göteborg, Göteborg, Sweden. guo-min@immuno.gu.se

ABSTRACT
Our results show that cytokines derived from macrophages play an important role in pathogenesis of arthritis triggered by CpG oligodinucleotide (CpG ODN). IL-12 is in this respect an important immunomodulator during the development of joint inflammation.

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Related in: MedlinePlus

Kinetics of synovial cytokine mRNA expression in knees of mice given an intra-articular injection (6 μg) of CpG ODN. (a) IFN-γ and IL-1β; (b) TNF-α and IL-12. Each bar represents the mean number and SEM of stained cells per mm2 in synovial tissue from four mice for each interval after inoculation.
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Figure 2: Kinetics of synovial cytokine mRNA expression in knees of mice given an intra-articular injection (6 μg) of CpG ODN. (a) IFN-γ and IL-1β; (b) TNF-α and IL-12. Each bar represents the mean number and SEM of stained cells per mm2 in synovial tissue from four mice for each interval after inoculation.

Mentions: Various workers have suggested that locally released cytokines are a key mediator of the inflammation and joint destruction observed in inflammatory arthritis [11,12,13,14,15]. To assess the local induction of cytokines, we analyzed the expression of their mRNA in synovia, using hybridization in situ. Figure 1 shows a typical result. None of the cytokine mRNAs studied were detected in the synovia of mice given intra-articular injections of calf-thymus DNA or phosphate-buffered saline solution. In contrast, after intra-articular inoculation with CpG ODN, the monocyte/macrophage-derived cytokines TNF-α, IL-1β, and IL-12 were induced rapidly, being detected within the first day. The expression of TNF-α mRNA peaked on day 3 and then decreased, whereas that of IL-1β mRNA rose on day 1 and remained high from then onwards. IL-12 mRNA rose to peak values between days 3 and 21. The Th1 cytokine IFN-γ mRNA was undetectable throughout the experiment (Fig. 2a,b).


Synovial cytokine mRNA expression during arthritis triggered by CpG motifs of bacterial DNA.

Deng GM, Tarkowski A - Arthritis Res. (2000)

Kinetics of synovial cytokine mRNA expression in knees of mice given an intra-articular injection (6 μg) of CpG ODN. (a) IFN-γ and IL-1β; (b) TNF-α and IL-12. Each bar represents the mean number and SEM of stained cells per mm2 in synovial tissue from four mice for each interval after inoculation.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC17824&req=5

Figure 2: Kinetics of synovial cytokine mRNA expression in knees of mice given an intra-articular injection (6 μg) of CpG ODN. (a) IFN-γ and IL-1β; (b) TNF-α and IL-12. Each bar represents the mean number and SEM of stained cells per mm2 in synovial tissue from four mice for each interval after inoculation.
Mentions: Various workers have suggested that locally released cytokines are a key mediator of the inflammation and joint destruction observed in inflammatory arthritis [11,12,13,14,15]. To assess the local induction of cytokines, we analyzed the expression of their mRNA in synovia, using hybridization in situ. Figure 1 shows a typical result. None of the cytokine mRNAs studied were detected in the synovia of mice given intra-articular injections of calf-thymus DNA or phosphate-buffered saline solution. In contrast, after intra-articular inoculation with CpG ODN, the monocyte/macrophage-derived cytokines TNF-α, IL-1β, and IL-12 were induced rapidly, being detected within the first day. The expression of TNF-α mRNA peaked on day 3 and then decreased, whereas that of IL-1β mRNA rose on day 1 and remained high from then onwards. IL-12 mRNA rose to peak values between days 3 and 21. The Th1 cytokine IFN-γ mRNA was undetectable throughout the experiment (Fig. 2a,b).

Bottom Line: Our results show that cytokines derived from macrophages play an important role in pathogenesis of arthritis triggered by CpG oligodinucleotide (CpG ODN).IL-12 is in this respect an important immunomodulator during the development of joint inflammation.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Rheumatology, University of Göteborg, Göteborg, Sweden. guo-min@immuno.gu.se

ABSTRACT
Our results show that cytokines derived from macrophages play an important role in pathogenesis of arthritis triggered by CpG oligodinucleotide (CpG ODN). IL-12 is in this respect an important immunomodulator during the development of joint inflammation.

Show MeSH
Related in: MedlinePlus