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Inhibition of vascular remodelling in a porcine coronary injury model by herbal extract XS0601.

Xu H, Shi D, Chen K - Chin Med (2006)

Bottom Line: XS0601 markedly inhibited proliferation of smooth muscle cells (SMCs) and transformation of SMCs from contractile to synthetic phenotype in neointima, inhibited hyperplasia-related indices of morphometric analysis and reduce late angiographic lumen loss.The reduction of the late angiographic lumen loss resulting from vascular remodelling was greater after XS0601 treatment.XS0601 markedly reduced angiographic late lumen loss resulting from intimal hyperplasia, vascular remodelling and XS0601 may be a potential agent to prevent restenosis after PCI.

View Article: PubMed Central - HTML - PubMed

Affiliation: National Integrative Medicine Centre for Cardiovascular Diseases, China-Japan Friendship Hospital, 2 Yinghuayuan East Street, Chaoyang District, Beijing 100029, China. xuhao2005@yahoo.com.cn

ABSTRACT

Background: Arterial remodelling is a major pathologic change of restenosis after percutaneous coronary intervention (PCI). Our previous studies showed that XS0601 (consisting of Chuangxingol and paeoniflorin) had some effects on the prevention of restenosis after PCI. Therefore, the purpose of this study was to examine whether and how its mechanism was related to the regulation of the arterial remodelling after endothelial injury by balloon dilation.

Methods: Twenty Chinese mini-pigs were randomized into four groups: control, probucol, low-dose XS0601 and high-dose XS0601 group before oversized balloon injury of the left anterior descending coronary arteries. Starting from two days before balloon injury, the mini-pigs in the treated group were administered with probucol (2 g/day) and XS0601 (0.02 g/kg/day for low dose; 0.04 g/kg/day for high dose) for four weeks after balloon injury. The animals receiving balloon injury alone were used as control. Morphometric and angiographic analysis of the injured arteries were performed.

Results: The contribution of intimal hyperplasia and arterial remodelling to angiographic late lumen loss was 41% and 59% respectively. XS0601 markedly inhibited proliferation of smooth muscle cells (SMCs) and transformation of SMCs from contractile to synthetic phenotype in neointima, inhibited hyperplasia-related indices of morphometric analysis and reduce late angiographic lumen loss. The reduction of the late angiographic lumen loss resulting from vascular remodelling was greater after XS0601 treatment.

Conclusion: Both intimal hyperplasia and vascular remodelling are attributed to late lumen loss in this porcine coronary injury model. XS0601 markedly reduced angiographic late lumen loss resulting from intimal hyperplasia, vascular remodelling and XS0601 may be a potential agent to prevent restenosis after PCI.

No MeSH data available.


Related in: MedlinePlus

Comparison of PCNA labelling index in neointima of injury coronary artery among groups. In HXS, a significant reduction of the proliferative activity in the neointimal layer was indicated by the PCNA labelling index (HXS 4.87% vs. Control 10.14%, P < 0.05).
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Figure 4: Comparison of PCNA labelling index in neointima of injury coronary artery among groups. In HXS, a significant reduction of the proliferative activity in the neointimal layer was indicated by the PCNA labelling index (HXS 4.87% vs. Control 10.14%, P < 0.05).

Mentions: In HXS, administration of XS was also associated with a reduction of the proliferative activity in the neointimal layer, as indicated by the PCNA labelling index (4.87% vs.10.14%, P < 0.05) (Figures 3 and 4). Although PCNA staining measures the total cell replication activity in the artery segment, injured arteries examined by transmission electron microscopy showed that the proliferating intima was composed primarily of SMCs.


Inhibition of vascular remodelling in a porcine coronary injury model by herbal extract XS0601.

Xu H, Shi D, Chen K - Chin Med (2006)

Comparison of PCNA labelling index in neointima of injury coronary artery among groups. In HXS, a significant reduction of the proliferative activity in the neointimal layer was indicated by the PCNA labelling index (HXS 4.87% vs. Control 10.14%, P < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC1761146&req=5

Figure 4: Comparison of PCNA labelling index in neointima of injury coronary artery among groups. In HXS, a significant reduction of the proliferative activity in the neointimal layer was indicated by the PCNA labelling index (HXS 4.87% vs. Control 10.14%, P < 0.05).
Mentions: In HXS, administration of XS was also associated with a reduction of the proliferative activity in the neointimal layer, as indicated by the PCNA labelling index (4.87% vs.10.14%, P < 0.05) (Figures 3 and 4). Although PCNA staining measures the total cell replication activity in the artery segment, injured arteries examined by transmission electron microscopy showed that the proliferating intima was composed primarily of SMCs.

Bottom Line: XS0601 markedly inhibited proliferation of smooth muscle cells (SMCs) and transformation of SMCs from contractile to synthetic phenotype in neointima, inhibited hyperplasia-related indices of morphometric analysis and reduce late angiographic lumen loss.The reduction of the late angiographic lumen loss resulting from vascular remodelling was greater after XS0601 treatment.XS0601 markedly reduced angiographic late lumen loss resulting from intimal hyperplasia, vascular remodelling and XS0601 may be a potential agent to prevent restenosis after PCI.

View Article: PubMed Central - HTML - PubMed

Affiliation: National Integrative Medicine Centre for Cardiovascular Diseases, China-Japan Friendship Hospital, 2 Yinghuayuan East Street, Chaoyang District, Beijing 100029, China. xuhao2005@yahoo.com.cn

ABSTRACT

Background: Arterial remodelling is a major pathologic change of restenosis after percutaneous coronary intervention (PCI). Our previous studies showed that XS0601 (consisting of Chuangxingol and paeoniflorin) had some effects on the prevention of restenosis after PCI. Therefore, the purpose of this study was to examine whether and how its mechanism was related to the regulation of the arterial remodelling after endothelial injury by balloon dilation.

Methods: Twenty Chinese mini-pigs were randomized into four groups: control, probucol, low-dose XS0601 and high-dose XS0601 group before oversized balloon injury of the left anterior descending coronary arteries. Starting from two days before balloon injury, the mini-pigs in the treated group were administered with probucol (2 g/day) and XS0601 (0.02 g/kg/day for low dose; 0.04 g/kg/day for high dose) for four weeks after balloon injury. The animals receiving balloon injury alone were used as control. Morphometric and angiographic analysis of the injured arteries were performed.

Results: The contribution of intimal hyperplasia and arterial remodelling to angiographic late lumen loss was 41% and 59% respectively. XS0601 markedly inhibited proliferation of smooth muscle cells (SMCs) and transformation of SMCs from contractile to synthetic phenotype in neointima, inhibited hyperplasia-related indices of morphometric analysis and reduce late angiographic lumen loss. The reduction of the late angiographic lumen loss resulting from vascular remodelling was greater after XS0601 treatment.

Conclusion: Both intimal hyperplasia and vascular remodelling are attributed to late lumen loss in this porcine coronary injury model. XS0601 markedly reduced angiographic late lumen loss resulting from intimal hyperplasia, vascular remodelling and XS0601 may be a potential agent to prevent restenosis after PCI.

No MeSH data available.


Related in: MedlinePlus