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Expression of insulin pathway genes during the period of caste determination in the honey bee, Apis mellifera.

Wheeler DE, Buck N, Evans JD - Insect Mol. Biol. (2006)

Bottom Line: We examined the expression of several genes of the insulin signalling pathway, which is central to regulation of growth based on nutrition.We found one insulin-like peptide expressed at very high levels in queen but not worker larvae.Also, the gene for an insulin receptor was expressed at higher levels in queen larvae during the 2nd larval instar.

View Article: PubMed Central - PubMed

Affiliation: Department of Entomology, University of Arizona, Tucson, AZ, USA. dewsants@ag.arizona.edu

ABSTRACT
Female honeybees have two castes, queens and workers. Developmental fate is determined by larval diet. Coding sequences made available through the Honey Bee Genome Sequencing Consortium allow for a pathway-based approach to understanding caste determination. We examined the expression of several genes of the insulin signalling pathway, which is central to regulation of growth based on nutrition. We found one insulin-like peptide expressed at very high levels in queen but not worker larvae. Also, the gene for an insulin receptor was expressed at higher levels in queen larvae during the 2nd larval instar. These results demonstrate that the insulin pathway is a compelling candidate for pursing the relationship between diet and downstream signals involved in caste determination and differentiation.

Show MeSH
Expression of an Am insulin/IGF receptor protein, AmInR-2. Expression is high in young grafting age larvae and reaches even higher levels in queen-destined larvae at 40 hours. Standard errors are shown only for queen and worker larvae for ease of viewing.
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fig05: Expression of an Am insulin/IGF receptor protein, AmInR-2. Expression is high in young grafting age larvae and reaches even higher levels in queen-destined larvae at 40 hours. Standard errors are shown only for queen and worker larvae for ease of viewing.

Mentions: Expression of insulin receptor gene AmIR-2 was high in undifferentiated first instar larvae, and reached an even higher level in queen larvae during the 2nd instar (Fig. 5). Worker levels were consistently low. By the 3rd instar (64 h) expression in normal queens and workers were low and similar. Switching larvae from worker to queen cells at any time (Q, WQ, and WWQ) provoked a suggestive increase in receptor expression.


Expression of insulin pathway genes during the period of caste determination in the honey bee, Apis mellifera.

Wheeler DE, Buck N, Evans JD - Insect Mol. Biol. (2006)

Expression of an Am insulin/IGF receptor protein, AmInR-2. Expression is high in young grafting age larvae and reaches even higher levels in queen-destined larvae at 40 hours. Standard errors are shown only for queen and worker larvae for ease of viewing.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC1761130&req=5

fig05: Expression of an Am insulin/IGF receptor protein, AmInR-2. Expression is high in young grafting age larvae and reaches even higher levels in queen-destined larvae at 40 hours. Standard errors are shown only for queen and worker larvae for ease of viewing.
Mentions: Expression of insulin receptor gene AmIR-2 was high in undifferentiated first instar larvae, and reached an even higher level in queen larvae during the 2nd instar (Fig. 5). Worker levels were consistently low. By the 3rd instar (64 h) expression in normal queens and workers were low and similar. Switching larvae from worker to queen cells at any time (Q, WQ, and WWQ) provoked a suggestive increase in receptor expression.

Bottom Line: We examined the expression of several genes of the insulin signalling pathway, which is central to regulation of growth based on nutrition.We found one insulin-like peptide expressed at very high levels in queen but not worker larvae.Also, the gene for an insulin receptor was expressed at higher levels in queen larvae during the 2nd larval instar.

View Article: PubMed Central - PubMed

Affiliation: Department of Entomology, University of Arizona, Tucson, AZ, USA. dewsants@ag.arizona.edu

ABSTRACT
Female honeybees have two castes, queens and workers. Developmental fate is determined by larval diet. Coding sequences made available through the Honey Bee Genome Sequencing Consortium allow for a pathway-based approach to understanding caste determination. We examined the expression of several genes of the insulin signalling pathway, which is central to regulation of growth based on nutrition. We found one insulin-like peptide expressed at very high levels in queen but not worker larvae. Also, the gene for an insulin receptor was expressed at higher levels in queen larvae during the 2nd larval instar. These results demonstrate that the insulin pathway is a compelling candidate for pursing the relationship between diet and downstream signals involved in caste determination and differentiation.

Show MeSH