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Inorganic phosphate nanorods are a novel fluorescent label in cell biology.

Patra CR, Bhattacharya R, Patra S, Basu S, Mukherjee P, Mukhopadhyay D - J Nanobiotechnology (2006)

Bottom Line: We report the first use of inorganic fluorescent lanthanide (europium and terbium) ortho phosphate [LnPO4.H2O, Ln = Eu and Tb] nanorods as a novel fluorescent label in cell biology.These nanorods, synthesized by the microwave technique, retain their fluorescent properties after internalization into human umbilical vein endothelial cells (HUVEC), 786-O cells, or renal carcinoma cells (RCC).The cellular internalization of these nanorods and their fluorescence properties were characterized by fluorescence spectroscopy (FS), differential interference contrast (DIC) microscopy, confocal microscopy, and transmission electron microscopy (TEM).

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, Mayo Clinic Cancer Center, Mayo Clinic, Rochester, Minnesota, USA. patra.chittaranjan@mayo.edu

ABSTRACT
We report the first use of inorganic fluorescent lanthanide (europium and terbium) ortho phosphate [LnPO4.H2O, Ln = Eu and Tb] nanorods as a novel fluorescent label in cell biology. These nanorods, synthesized by the microwave technique, retain their fluorescent properties after internalization into human umbilical vein endothelial cells (HUVEC), 786-O cells, or renal carcinoma cells (RCC). The cellular internalization of these nanorods and their fluorescence properties were characterized by fluorescence spectroscopy (FS), differential interference contrast (DIC) microscopy, confocal microscopy, and transmission electron microscopy (TEM). At concentrations up to 50 microg/ml, the use of [3H]-thymidine incorporation assays, apoptosis assays (TUNEL), and trypan blue exclusion illustrated the non-toxic nature of these nanorods, a major advantage over traditional organic dyes.

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TEM images of as-synthesized (A-B) EuPO4·H2O nanorods and (C-D) TbPO4·H2O nanorods with different magnifications, respectively.
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Figure 1: TEM images of as-synthesized (A-B) EuPO4·H2O nanorods and (C-D) TbPO4·H2O nanorods with different magnifications, respectively.

Mentions: The morphologies of LnPO4·H2O [Ln = Eu and Tb] nanomaterials were further characterized by transmission electron microscopy (TEM) at different magnifications (Figure 1A–D). The TEM images of as-synthesized products clearly showed that EuPO4·H2O material (Figure 1A–B) entirely consists of nanorods [6 to 8 nm in diameter and 100 to 300 nm in length] and TbPO4·H2O products (Figure 1C–D) were a mixture of two rod types in micrometer size (small rods at 0.5 to 1.5 μm in length and 6 to 8 nm in width and bigger rods at 1.1 to 2.2 μm in length and 80 to 130 nm in width).


Inorganic phosphate nanorods are a novel fluorescent label in cell biology.

Patra CR, Bhattacharya R, Patra S, Basu S, Mukherjee P, Mukhopadhyay D - J Nanobiotechnology (2006)

TEM images of as-synthesized (A-B) EuPO4·H2O nanorods and (C-D) TbPO4·H2O nanorods with different magnifications, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC1636656&req=5

Figure 1: TEM images of as-synthesized (A-B) EuPO4·H2O nanorods and (C-D) TbPO4·H2O nanorods with different magnifications, respectively.
Mentions: The morphologies of LnPO4·H2O [Ln = Eu and Tb] nanomaterials were further characterized by transmission electron microscopy (TEM) at different magnifications (Figure 1A–D). The TEM images of as-synthesized products clearly showed that EuPO4·H2O material (Figure 1A–B) entirely consists of nanorods [6 to 8 nm in diameter and 100 to 300 nm in length] and TbPO4·H2O products (Figure 1C–D) were a mixture of two rod types in micrometer size (small rods at 0.5 to 1.5 μm in length and 6 to 8 nm in width and bigger rods at 1.1 to 2.2 μm in length and 80 to 130 nm in width).

Bottom Line: We report the first use of inorganic fluorescent lanthanide (europium and terbium) ortho phosphate [LnPO4.H2O, Ln = Eu and Tb] nanorods as a novel fluorescent label in cell biology.These nanorods, synthesized by the microwave technique, retain their fluorescent properties after internalization into human umbilical vein endothelial cells (HUVEC), 786-O cells, or renal carcinoma cells (RCC).The cellular internalization of these nanorods and their fluorescence properties were characterized by fluorescence spectroscopy (FS), differential interference contrast (DIC) microscopy, confocal microscopy, and transmission electron microscopy (TEM).

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, Mayo Clinic Cancer Center, Mayo Clinic, Rochester, Minnesota, USA. patra.chittaranjan@mayo.edu

ABSTRACT
We report the first use of inorganic fluorescent lanthanide (europium and terbium) ortho phosphate [LnPO4.H2O, Ln = Eu and Tb] nanorods as a novel fluorescent label in cell biology. These nanorods, synthesized by the microwave technique, retain their fluorescent properties after internalization into human umbilical vein endothelial cells (HUVEC), 786-O cells, or renal carcinoma cells (RCC). The cellular internalization of these nanorods and their fluorescence properties were characterized by fluorescence spectroscopy (FS), differential interference contrast (DIC) microscopy, confocal microscopy, and transmission electron microscopy (TEM). At concentrations up to 50 microg/ml, the use of [3H]-thymidine incorporation assays, apoptosis assays (TUNEL), and trypan blue exclusion illustrated the non-toxic nature of these nanorods, a major advantage over traditional organic dyes.

No MeSH data available.


Related in: MedlinePlus