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Different prion disease phenotypes result from inoculation of cattle with two temporally separated sources of sheep scrapie from Great Britain.

Konold T, Lee YH, Stack MJ, Horrocks C, Green RB, Chaplin M, Simmons MM, Hawkins SA, Lockey R, Spiropoulos J, Wilesmith JW, Wells GA - BMC Vet. Res. (2006)

Bottom Line: Disease occurred in 16 cattle, nine inoculated with the pre-1975 inoculum and seven inoculated with the post-1990 inoculum, with four cattle still alive at 83 months post challenge (as at June 2006).The different inocula produced predominantly two different disease phenotypes as determined by histopathological, immunohistochemical and Western immunoblotting methods and biological characterisation on transmission to mice, neither of which was identical to BSE.The study has demonstrated that cattle inoculated with different pooled scrapie sources can develop different prion disease phenotypes, which were not consistent with the phenotype of BSE of cattle and whose isolates did not have the strain typing characteristics of the BSE agent on transmission to mice.

View Article: PubMed Central - HTML - PubMed

Affiliation: Veterinary Laboratories Agency Weybridge, New Haw, Addlestone, UK. t.konold@vla.defra.gsi.gov.uk

ABSTRACT

Background: Given the theoretical proposal that bovine spongiform encephalopathy (BSE) could have originated from sheep scrapie, this study investigated the pathogenicity for cattle, by intracerebral (i.c.) inoculation, of two pools of scrapie agents sourced in Great Britain before and during the BSE epidemic. Two groups of ten cattle were each inoculated with pools of brain material from sheep scrapie cases collected prior to 1975 and after 1990. Control groups comprised five cattle inoculated with sheep brain free from scrapie, five cattle inoculated with saline, and for comparison with BSE, naturally infected cattle and cattle i.c. inoculated with BSE brainstem homogenate from a parallel study. Phenotypic characterisation of the disease forms transmitted to cattle was conducted by morphological, immunohistochemical, biochemical and biological methods.

Results: Disease occurred in 16 cattle, nine inoculated with the pre-1975 inoculum and seven inoculated with the post-1990 inoculum, with four cattle still alive at 83 months post challenge (as at June 2006). The different inocula produced predominantly two different disease phenotypes as determined by histopathological, immunohistochemical and Western immunoblotting methods and biological characterisation on transmission to mice, neither of which was identical to BSE. Whilst the disease presentation was uniform in all scrapie-affected cattle of the pre-1975 group, the post-1990 inoculum produced a more variable disease, with two animals sharing immunohistochemical and molecular profile characteristics with animals in the pre-1975 group.

Conclusion: The study has demonstrated that cattle inoculated with different pooled scrapie sources can develop different prion disease phenotypes, which were not consistent with the phenotype of BSE of cattle and whose isolates did not have the strain typing characteristics of the BSE agent on transmission to mice.

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Tree diagram after cluster analysis of the vacuolation scores in selected neuroanatomical brain areas. A) Comparison of the profiles in cattle. B) Comparison of the profiles in mice. Natural BSE pool inoculum codes: study/inoculum.
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Figure 2: Tree diagram after cluster analysis of the vacuolation scores in selected neuroanatomical brain areas. A) Comparison of the profiles in cattle. B) Comparison of the profiles in mice. Natural BSE pool inoculum codes: study/inoculum.

Mentions: The lesion profiles of both groups inoculated with scrapie material and comparisons with corresponding data from brains of cattle i.c. and naturally infected with BSE are given in Figure 1. Both scrapie inocula appear to produce a similar lesion profile in cattle with similar vacuolation scores in most of the 17 neuroanatomical areas and only minor differences (reduced vacuolation scores in vestibular nucleus complex, cochlear nucleus, caudate nucleus and putamen in the pre-1975 group). The most significant differences from natural and experimental (i.c.) BSE were observed in areas 1, 2 and 7 (markedly decreased vacuolation scores in the nucleus of the solitary tract, the spinal tract nucleus of the trigeminal nerve and central grey matter). Cluster analysis separated the pre-1975 and post-1990 group from natural and experimental BSE (Figure 2A).


Different prion disease phenotypes result from inoculation of cattle with two temporally separated sources of sheep scrapie from Great Britain.

Konold T, Lee YH, Stack MJ, Horrocks C, Green RB, Chaplin M, Simmons MM, Hawkins SA, Lockey R, Spiropoulos J, Wilesmith JW, Wells GA - BMC Vet. Res. (2006)

Tree diagram after cluster analysis of the vacuolation scores in selected neuroanatomical brain areas. A) Comparison of the profiles in cattle. B) Comparison of the profiles in mice. Natural BSE pool inoculum codes: study/inoculum.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC1636635&req=5

Figure 2: Tree diagram after cluster analysis of the vacuolation scores in selected neuroanatomical brain areas. A) Comparison of the profiles in cattle. B) Comparison of the profiles in mice. Natural BSE pool inoculum codes: study/inoculum.
Mentions: The lesion profiles of both groups inoculated with scrapie material and comparisons with corresponding data from brains of cattle i.c. and naturally infected with BSE are given in Figure 1. Both scrapie inocula appear to produce a similar lesion profile in cattle with similar vacuolation scores in most of the 17 neuroanatomical areas and only minor differences (reduced vacuolation scores in vestibular nucleus complex, cochlear nucleus, caudate nucleus and putamen in the pre-1975 group). The most significant differences from natural and experimental (i.c.) BSE were observed in areas 1, 2 and 7 (markedly decreased vacuolation scores in the nucleus of the solitary tract, the spinal tract nucleus of the trigeminal nerve and central grey matter). Cluster analysis separated the pre-1975 and post-1990 group from natural and experimental BSE (Figure 2A).

Bottom Line: Disease occurred in 16 cattle, nine inoculated with the pre-1975 inoculum and seven inoculated with the post-1990 inoculum, with four cattle still alive at 83 months post challenge (as at June 2006).The different inocula produced predominantly two different disease phenotypes as determined by histopathological, immunohistochemical and Western immunoblotting methods and biological characterisation on transmission to mice, neither of which was identical to BSE.The study has demonstrated that cattle inoculated with different pooled scrapie sources can develop different prion disease phenotypes, which were not consistent with the phenotype of BSE of cattle and whose isolates did not have the strain typing characteristics of the BSE agent on transmission to mice.

View Article: PubMed Central - HTML - PubMed

Affiliation: Veterinary Laboratories Agency Weybridge, New Haw, Addlestone, UK. t.konold@vla.defra.gsi.gov.uk

ABSTRACT

Background: Given the theoretical proposal that bovine spongiform encephalopathy (BSE) could have originated from sheep scrapie, this study investigated the pathogenicity for cattle, by intracerebral (i.c.) inoculation, of two pools of scrapie agents sourced in Great Britain before and during the BSE epidemic. Two groups of ten cattle were each inoculated with pools of brain material from sheep scrapie cases collected prior to 1975 and after 1990. Control groups comprised five cattle inoculated with sheep brain free from scrapie, five cattle inoculated with saline, and for comparison with BSE, naturally infected cattle and cattle i.c. inoculated with BSE brainstem homogenate from a parallel study. Phenotypic characterisation of the disease forms transmitted to cattle was conducted by morphological, immunohistochemical, biochemical and biological methods.

Results: Disease occurred in 16 cattle, nine inoculated with the pre-1975 inoculum and seven inoculated with the post-1990 inoculum, with four cattle still alive at 83 months post challenge (as at June 2006). The different inocula produced predominantly two different disease phenotypes as determined by histopathological, immunohistochemical and Western immunoblotting methods and biological characterisation on transmission to mice, neither of which was identical to BSE. Whilst the disease presentation was uniform in all scrapie-affected cattle of the pre-1975 group, the post-1990 inoculum produced a more variable disease, with two animals sharing immunohistochemical and molecular profile characteristics with animals in the pre-1975 group.

Conclusion: The study has demonstrated that cattle inoculated with different pooled scrapie sources can develop different prion disease phenotypes, which were not consistent with the phenotype of BSE of cattle and whose isolates did not have the strain typing characteristics of the BSE agent on transmission to mice.

Show MeSH
Related in: MedlinePlus