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Gamma protocadherin expression in the embryonic chick nervous system.

Cronin KD, Capehart AA - Int. J. Biol. Sci. (2006)

Bottom Line: Protocadherin gamma (pcdh-gamma) family expression was examined in the embryonic chick central nervous system by in situ hybridization.Transcripts were visualized in discrete regions of fore-, mid-, and hindbrain at stages 23 and 25 and in spinal cord and optic lobe at stages 27 and 43, respectively.Results suggest that pcdh-gamma may function cooperatively with other cell adhesion molecules in neuronal differentiation and establishment of neural networks in several areas of the developing brain, particularly regions involved in visual processing.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, East Carolina University, Greenville, North Carolina 27858, USA.

ABSTRACT
Protocadherin gamma (pcdh-gamma) family expression was examined in the embryonic chick central nervous system by in situ hybridization. Transcripts were visualized in discrete regions of fore-, mid-, and hindbrain at stages 23 and 25 and in spinal cord and optic lobe at stages 27 and 43, respectively. Results suggest that pcdh-gamma may function cooperatively with other cell adhesion molecules in neuronal differentiation and establishment of neural networks in several areas of the developing brain, particularly regions involved in visual processing.

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Pcdh-γ in the stage 27 spinal cord. (a) Localization of pcdh-γ transcripts in the dorsal horn, floorplate (arrow) and dorsal root ganglion (arrowhead). Staining of interneurons in the developing dorsal horn (boxed area) is shown in higher magnification in (b). Higher magnification image of floorplate pcdh-γ expression is shown in (c). (d) Section incubated with control probe shows lack of signal in spinal cord and dorsal root ganglia. Scale bars = 250 μm for panels 4a & d, 25 μm for 4b and 50 μm for 4c.
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Figure 4: Pcdh-γ in the stage 27 spinal cord. (a) Localization of pcdh-γ transcripts in the dorsal horn, floorplate (arrow) and dorsal root ganglion (arrowhead). Staining of interneurons in the developing dorsal horn (boxed area) is shown in higher magnification in (b). Higher magnification image of floorplate pcdh-γ expression is shown in (c). (d) Section incubated with control probe shows lack of signal in spinal cord and dorsal root ganglia. Scale bars = 250 μm for panels 4a & d, 25 μm for 4b and 50 μm for 4c.

Mentions: In the developing spinal cord at stage 27, pcdh-γ family transcripts were present, particularly in the dorsal horn and floorplate regions (Figure 4a-c). Expression was also observed in dorsal root ganglia (DRG). Expression in the dorsal horn and DRG is consistent with that reported for a single member of the pcdh-γA subfamily in chick, γA-like pcdh (pcdh-γA5; 14), and suggests a role for one or more members of the pcdh-γ family in interaction between interneurons and the DRG during establishment of peripheral sensory connections with the CNS. In embryonic mouse spinal cord, expression of the pcdh-γ family was widespread in gray matter, but preferentially associated with the dorsal horn in the adult 20 and appropriate spinal cord interneuron survival and synapse formation or maintenance have been demonstrated dependent on the pcdh-γ family 8, 9. In addition, pcdh-γ protein was demonstrated in early axonal and dendritic growth cones of cultured murine e12.5 spinal cord neurons, later becoming progressively restricted to the somatodendritic compartment indicating that pcdh-γ members may also participate in neuronal maturation and neurite outgrowth 20. Expression in the chick floorplate as seen with the pcdh-γ constant region in situ hybridization probe used in the present study was not observed earlier for γA-like pcdh 14, but in mouse, pcdh-γ isoforms and pcdh 15 have been reported in floorplates of brain ventricles 24 and the neural tube 25. The difference in chick neural tube localization between the common γ family probe used in the present study and isoform specific γA-like pcdh probe reported previously supports the notion that more than one pcdh-γ family member is expressed in the developing chick spinal cord. Interestingly, chick OL-pcdh is found only in the motor column of the ventral horn 12 further suggesting that multiple pcdh families may function in different spinal cord compartments.


Gamma protocadherin expression in the embryonic chick nervous system.

Cronin KD, Capehart AA - Int. J. Biol. Sci. (2006)

Pcdh-γ in the stage 27 spinal cord. (a) Localization of pcdh-γ transcripts in the dorsal horn, floorplate (arrow) and dorsal root ganglion (arrowhead). Staining of interneurons in the developing dorsal horn (boxed area) is shown in higher magnification in (b). Higher magnification image of floorplate pcdh-γ expression is shown in (c). (d) Section incubated with control probe shows lack of signal in spinal cord and dorsal root ganglia. Scale bars = 250 μm for panels 4a & d, 25 μm for 4b and 50 μm for 4c.
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Figure 4: Pcdh-γ in the stage 27 spinal cord. (a) Localization of pcdh-γ transcripts in the dorsal horn, floorplate (arrow) and dorsal root ganglion (arrowhead). Staining of interneurons in the developing dorsal horn (boxed area) is shown in higher magnification in (b). Higher magnification image of floorplate pcdh-γ expression is shown in (c). (d) Section incubated with control probe shows lack of signal in spinal cord and dorsal root ganglia. Scale bars = 250 μm for panels 4a & d, 25 μm for 4b and 50 μm for 4c.
Mentions: In the developing spinal cord at stage 27, pcdh-γ family transcripts were present, particularly in the dorsal horn and floorplate regions (Figure 4a-c). Expression was also observed in dorsal root ganglia (DRG). Expression in the dorsal horn and DRG is consistent with that reported for a single member of the pcdh-γA subfamily in chick, γA-like pcdh (pcdh-γA5; 14), and suggests a role for one or more members of the pcdh-γ family in interaction between interneurons and the DRG during establishment of peripheral sensory connections with the CNS. In embryonic mouse spinal cord, expression of the pcdh-γ family was widespread in gray matter, but preferentially associated with the dorsal horn in the adult 20 and appropriate spinal cord interneuron survival and synapse formation or maintenance have been demonstrated dependent on the pcdh-γ family 8, 9. In addition, pcdh-γ protein was demonstrated in early axonal and dendritic growth cones of cultured murine e12.5 spinal cord neurons, later becoming progressively restricted to the somatodendritic compartment indicating that pcdh-γ members may also participate in neuronal maturation and neurite outgrowth 20. Expression in the chick floorplate as seen with the pcdh-γ constant region in situ hybridization probe used in the present study was not observed earlier for γA-like pcdh 14, but in mouse, pcdh-γ isoforms and pcdh 15 have been reported in floorplates of brain ventricles 24 and the neural tube 25. The difference in chick neural tube localization between the common γ family probe used in the present study and isoform specific γA-like pcdh probe reported previously supports the notion that more than one pcdh-γ family member is expressed in the developing chick spinal cord. Interestingly, chick OL-pcdh is found only in the motor column of the ventral horn 12 further suggesting that multiple pcdh families may function in different spinal cord compartments.

Bottom Line: Protocadherin gamma (pcdh-gamma) family expression was examined in the embryonic chick central nervous system by in situ hybridization.Transcripts were visualized in discrete regions of fore-, mid-, and hindbrain at stages 23 and 25 and in spinal cord and optic lobe at stages 27 and 43, respectively.Results suggest that pcdh-gamma may function cooperatively with other cell adhesion molecules in neuronal differentiation and establishment of neural networks in several areas of the developing brain, particularly regions involved in visual processing.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, East Carolina University, Greenville, North Carolina 27858, USA.

ABSTRACT
Protocadherin gamma (pcdh-gamma) family expression was examined in the embryonic chick central nervous system by in situ hybridization. Transcripts were visualized in discrete regions of fore-, mid-, and hindbrain at stages 23 and 25 and in spinal cord and optic lobe at stages 27 and 43, respectively. Results suggest that pcdh-gamma may function cooperatively with other cell adhesion molecules in neuronal differentiation and establishment of neural networks in several areas of the developing brain, particularly regions involved in visual processing.

Show MeSH
Related in: MedlinePlus