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Gamma protocadherin expression in the embryonic chick nervous system.

Cronin KD, Capehart AA - Int. J. Biol. Sci. (2006)

Bottom Line: Protocadherin gamma (pcdh-gamma) family expression was examined in the embryonic chick central nervous system by in situ hybridization.Transcripts were visualized in discrete regions of fore-, mid-, and hindbrain at stages 23 and 25 and in spinal cord and optic lobe at stages 27 and 43, respectively.Results suggest that pcdh-gamma may function cooperatively with other cell adhesion molecules in neuronal differentiation and establishment of neural networks in several areas of the developing brain, particularly regions involved in visual processing.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, East Carolina University, Greenville, North Carolina 27858, USA.

ABSTRACT
Protocadherin gamma (pcdh-gamma) family expression was examined in the embryonic chick central nervous system by in situ hybridization. Transcripts were visualized in discrete regions of fore-, mid-, and hindbrain at stages 23 and 25 and in spinal cord and optic lobe at stages 27 and 43, respectively. Results suggest that pcdh-gamma may function cooperatively with other cell adhesion molecules in neuronal differentiation and establishment of neural networks in several areas of the developing brain, particularly regions involved in visual processing.

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Pcdh-γ in the dorsal metencephalon (arrow) at stage 25. Separation of future pons and cerebellum is marked by the isthmus (asterisk). Scale bar = 250 μm.
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Figure 3: Pcdh-γ in the dorsal metencephalon (arrow) at stage 25. Separation of future pons and cerebellum is marked by the isthmus (asterisk). Scale bar = 250 μm.

Mentions: At stage 25 the chick brain has entered a transitional phase between initial morphogenesis of brain compartments and establishment of the terminal cytoarchitecture comprising nuclei and fiber tracts 17. Pcdh-γ transcripts at stage 25 were largely expressed in the ependymal layer extending into the mantle zone within different sites of the fore-, mid- and hindbrain (Figures 2 and 3) consistent with a role in early differentiation of neuronal subsets. In the telencephalon at this stage of development, mitotic cells of the ependymal layer and incipient neuroblasts of the mantle zone merge over a large area 17 and expression was observed primarily in the dorsal region of the developing cerebrum (pallium). Accumulation of pcdh-γ transcripts was particularly strong within the telodiencephalic eminence (Figure 2b) which merges with the corpus striatum and forms a boundary between the tele- and diencephalon 17. In the diencephalon, pcdh-γ transcripts were also distributed primarily within the dorsal region (future dorsal thalamus and pretectal nuclei) with a notable absence of expression along its ventral length. In the mouse forebrain at mid- to late gestation and in the adult, pcdh-γ mRNA expression was shown in different regions, particularly in hippocampus and cerebral cortex 8, 20, 21, 23, 24. In chick, OL-pcdh was localized in ventral forebrain at early stages 12 and later associated particularly with visual brain nuclei in areas such as thalamus 11 while the pcdh-α family exhibited moderate and widespread expression in the forebrain of the later stage embryonic chick 13.


Gamma protocadherin expression in the embryonic chick nervous system.

Cronin KD, Capehart AA - Int. J. Biol. Sci. (2006)

Pcdh-γ in the dorsal metencephalon (arrow) at stage 25. Separation of future pons and cerebellum is marked by the isthmus (asterisk). Scale bar = 250 μm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC1622892&req=5

Figure 3: Pcdh-γ in the dorsal metencephalon (arrow) at stage 25. Separation of future pons and cerebellum is marked by the isthmus (asterisk). Scale bar = 250 μm.
Mentions: At stage 25 the chick brain has entered a transitional phase between initial morphogenesis of brain compartments and establishment of the terminal cytoarchitecture comprising nuclei and fiber tracts 17. Pcdh-γ transcripts at stage 25 were largely expressed in the ependymal layer extending into the mantle zone within different sites of the fore-, mid- and hindbrain (Figures 2 and 3) consistent with a role in early differentiation of neuronal subsets. In the telencephalon at this stage of development, mitotic cells of the ependymal layer and incipient neuroblasts of the mantle zone merge over a large area 17 and expression was observed primarily in the dorsal region of the developing cerebrum (pallium). Accumulation of pcdh-γ transcripts was particularly strong within the telodiencephalic eminence (Figure 2b) which merges with the corpus striatum and forms a boundary between the tele- and diencephalon 17. In the diencephalon, pcdh-γ transcripts were also distributed primarily within the dorsal region (future dorsal thalamus and pretectal nuclei) with a notable absence of expression along its ventral length. In the mouse forebrain at mid- to late gestation and in the adult, pcdh-γ mRNA expression was shown in different regions, particularly in hippocampus and cerebral cortex 8, 20, 21, 23, 24. In chick, OL-pcdh was localized in ventral forebrain at early stages 12 and later associated particularly with visual brain nuclei in areas such as thalamus 11 while the pcdh-α family exhibited moderate and widespread expression in the forebrain of the later stage embryonic chick 13.

Bottom Line: Protocadherin gamma (pcdh-gamma) family expression was examined in the embryonic chick central nervous system by in situ hybridization.Transcripts were visualized in discrete regions of fore-, mid-, and hindbrain at stages 23 and 25 and in spinal cord and optic lobe at stages 27 and 43, respectively.Results suggest that pcdh-gamma may function cooperatively with other cell adhesion molecules in neuronal differentiation and establishment of neural networks in several areas of the developing brain, particularly regions involved in visual processing.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, East Carolina University, Greenville, North Carolina 27858, USA.

ABSTRACT
Protocadherin gamma (pcdh-gamma) family expression was examined in the embryonic chick central nervous system by in situ hybridization. Transcripts were visualized in discrete regions of fore-, mid-, and hindbrain at stages 23 and 25 and in spinal cord and optic lobe at stages 27 and 43, respectively. Results suggest that pcdh-gamma may function cooperatively with other cell adhesion molecules in neuronal differentiation and establishment of neural networks in several areas of the developing brain, particularly regions involved in visual processing.

Show MeSH
Related in: MedlinePlus