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Gamma protocadherin expression in the embryonic chick nervous system.

Cronin KD, Capehart AA - Int. J. Biol. Sci. (2006)

Bottom Line: Protocadherin gamma (pcdh-gamma) family expression was examined in the embryonic chick central nervous system by in situ hybridization.Transcripts were visualized in discrete regions of fore-, mid-, and hindbrain at stages 23 and 25 and in spinal cord and optic lobe at stages 27 and 43, respectively.Results suggest that pcdh-gamma may function cooperatively with other cell adhesion molecules in neuronal differentiation and establishment of neural networks in several areas of the developing brain, particularly regions involved in visual processing.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, East Carolina University, Greenville, North Carolina 27858, USA.

ABSTRACT
Protocadherin gamma (pcdh-gamma) family expression was examined in the embryonic chick central nervous system by in situ hybridization. Transcripts were visualized in discrete regions of fore-, mid-, and hindbrain at stages 23 and 25 and in spinal cord and optic lobe at stages 27 and 43, respectively. Results suggest that pcdh-gamma may function cooperatively with other cell adhesion molecules in neuronal differentiation and establishment of neural networks in several areas of the developing brain, particularly regions involved in visual processing.

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Pcdh-γ in the stage 23 hindbrain. (a) Pcdh-γ transcripts are localized primarily within the mantle zone (arrow). Anterior-most neural tissue on the right side of panel is slightly posterior to the midbrain/hindbrain boundary. The hindbrain roofplate is indicated by the arrowhead. Boxed area of mantle zone is shown in higher magnification in (b). (c) Corresponding region of ventral hindbrain shows lack of signal with sense control probe. Scale bars = 250 μm for panels 1a & c and 25 μm for 1b.
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Figure 1: Pcdh-γ in the stage 23 hindbrain. (a) Pcdh-γ transcripts are localized primarily within the mantle zone (arrow). Anterior-most neural tissue on the right side of panel is slightly posterior to the midbrain/hindbrain boundary. The hindbrain roofplate is indicated by the arrowhead. Boxed area of mantle zone is shown in higher magnification in (b). (c) Corresponding region of ventral hindbrain shows lack of signal with sense control probe. Scale bars = 250 μm for panels 1a & c and 25 μm for 1b.

Mentions: Significant expression of pcdh-γ was first detected in the chick CNS at stage 23 particularly within cells along the ventral aspect of the myelencephalon (Figure 1). Little expression of pcdh-γ was observed in different regions of the CNS at earlier stages (stages 18 and 21). At stage 23, transcripts were limited to larger cells in the hindbrain, likely neuronal cell bodies, primarily in the mantle zone. Arrangement of pcdh-γ labeled cells into identifiable nuclei (e.g., cranial nerve motor nuclei) at this stage could not be ascertained. In mouse, levels of pcdh-γ protein were low in adult hindbrain by Western blot 20, but present in the embryonic hindbrain 21 as observed in early chick in the present study. Expression of OL-protocadherin (OL-pcdh), the single member of another of the approximately seventeen different pcdh families described thus far 6, 22, was also reported in the embryonic chick hindbrain 12 with the protein localized predominately in axons along its ventral length. Strong expression of OL-pcdh protein in axon fibers and inability to localize OL-pcdh at cell junctions led to the suggestion that OL-pcdh functions primarily in axon growth or guidance 12 rather than in neuronal differentiation and synaptogenesis as proposed for pcdh-γ 21.


Gamma protocadherin expression in the embryonic chick nervous system.

Cronin KD, Capehart AA - Int. J. Biol. Sci. (2006)

Pcdh-γ in the stage 23 hindbrain. (a) Pcdh-γ transcripts are localized primarily within the mantle zone (arrow). Anterior-most neural tissue on the right side of panel is slightly posterior to the midbrain/hindbrain boundary. The hindbrain roofplate is indicated by the arrowhead. Boxed area of mantle zone is shown in higher magnification in (b). (c) Corresponding region of ventral hindbrain shows lack of signal with sense control probe. Scale bars = 250 μm for panels 1a & c and 25 μm for 1b.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC1622892&req=5

Figure 1: Pcdh-γ in the stage 23 hindbrain. (a) Pcdh-γ transcripts are localized primarily within the mantle zone (arrow). Anterior-most neural tissue on the right side of panel is slightly posterior to the midbrain/hindbrain boundary. The hindbrain roofplate is indicated by the arrowhead. Boxed area of mantle zone is shown in higher magnification in (b). (c) Corresponding region of ventral hindbrain shows lack of signal with sense control probe. Scale bars = 250 μm for panels 1a & c and 25 μm for 1b.
Mentions: Significant expression of pcdh-γ was first detected in the chick CNS at stage 23 particularly within cells along the ventral aspect of the myelencephalon (Figure 1). Little expression of pcdh-γ was observed in different regions of the CNS at earlier stages (stages 18 and 21). At stage 23, transcripts were limited to larger cells in the hindbrain, likely neuronal cell bodies, primarily in the mantle zone. Arrangement of pcdh-γ labeled cells into identifiable nuclei (e.g., cranial nerve motor nuclei) at this stage could not be ascertained. In mouse, levels of pcdh-γ protein were low in adult hindbrain by Western blot 20, but present in the embryonic hindbrain 21 as observed in early chick in the present study. Expression of OL-protocadherin (OL-pcdh), the single member of another of the approximately seventeen different pcdh families described thus far 6, 22, was also reported in the embryonic chick hindbrain 12 with the protein localized predominately in axons along its ventral length. Strong expression of OL-pcdh protein in axon fibers and inability to localize OL-pcdh at cell junctions led to the suggestion that OL-pcdh functions primarily in axon growth or guidance 12 rather than in neuronal differentiation and synaptogenesis as proposed for pcdh-γ 21.

Bottom Line: Protocadherin gamma (pcdh-gamma) family expression was examined in the embryonic chick central nervous system by in situ hybridization.Transcripts were visualized in discrete regions of fore-, mid-, and hindbrain at stages 23 and 25 and in spinal cord and optic lobe at stages 27 and 43, respectively.Results suggest that pcdh-gamma may function cooperatively with other cell adhesion molecules in neuronal differentiation and establishment of neural networks in several areas of the developing brain, particularly regions involved in visual processing.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, East Carolina University, Greenville, North Carolina 27858, USA.

ABSTRACT
Protocadherin gamma (pcdh-gamma) family expression was examined in the embryonic chick central nervous system by in situ hybridization. Transcripts were visualized in discrete regions of fore-, mid-, and hindbrain at stages 23 and 25 and in spinal cord and optic lobe at stages 27 and 43, respectively. Results suggest that pcdh-gamma may function cooperatively with other cell adhesion molecules in neuronal differentiation and establishment of neural networks in several areas of the developing brain, particularly regions involved in visual processing.

Show MeSH
Related in: MedlinePlus