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Uptake of the glycosphingolipid sulfatide in the gastrointestinal tract and pancreas in vivo and in isolated islets of Langerhans.

Blomqvist M, Osterbye T, Månsson JE, Buschard K, Fredman P - Lipids Health Dis (2006)

Bottom Line: The glycosphingolipid sulfatide has previously been found in several mammalian tissues, but information on the uptake of exogenously administered sulfatide in different organs in vivo is limited.Exogenously administered sulfatide was found in pancreas after i.p, but not after oral administration.Our study supports a selective uptake and/or preservation of sulfatide in the gastrointestinal tract after oral administration and with emphasises on pancreatic sulfatide uptake, i.p. administration results in sulfatide at relevant location.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Neuroscience and Physiology, Psychiatry and Neurochemistry Section, The Sahlgrenska Academy at Göteborg University, Sahlgrenska University Hospital/Mölndal, SE-431 80 Mölndal, Sweden. maria.blomqvist@gu.se

ABSTRACT

Background: The glycosphingolipid sulfatide has previously been found in several mammalian tissues, but information on the uptake of exogenously administered sulfatide in different organs in vivo is limited. In pancreatic beta cells, sulfatide has been shown to be involved in insulin processing and secretion in vitro. In this study, we examined the uptake of exogenously administered sulfatide and its distribution to the pancreatic beta cells. This might encourage future studies of the function(s) of sulfatide in beta cell physiology in vivo. Radioactive sulfatide was given orally to mice whereafter the uptake of sulfatide in the gastrointestinal tract and subsequent delivery to the pancreas was examined. Sulfatide uptake in pancreas was also studied in vivo by i.p. administration of radioactive sulfatide in mice, and in vitro in isolated rat islets. Isolated tissue/islets were analysed by scintillation counting, autoradiography and thin-layer chromatography-ELISA.

Results: Sulfatide was taken up in the gastrointestinal tract for degradation or further transport to other organs. A selective uptake of short chain and/or hydroxylated sulfatide fatty acid isoforms was observed in the small intestine. Exogenously administered sulfatide was found in pancreas after i.p, but not after oral administration. The in vitro studies in isolated rat islets support that sulfatide, independently of its fatty acid length, is endocytosed and metabolised by pancreatic islets.

Conclusion: Our study supports a selective uptake and/or preservation of sulfatide in the gastrointestinal tract after oral administration and with emphasises on pancreatic sulfatide uptake, i.p. administration results in sulfatide at relevant location.

Show MeSH
Sulfatide in pancreas of ob/ob mice. Thin layer chromatography of sulfatide in whole pancreas isolated from ob/ob mice given C16:0 sulfatide. The extraction and lipid isolation procedure as well as the TLC-ELISA method are described under "Methods". Lipids corresponding to approximately 3–4 mg tissue protein were applied (one representative plate is shown). Lane 1, seminolipid (120 pmol); lane 2, sulfated lactosylceramide (20 pmol); lane 3, sulfatide (40 pmol); lane 4, C16:0 sulfatide standard (25 pmol); lane 5, 6 hours after administration; lane 6, 24 hours after administration and lane 7, sulfatide isolated from untreated ob/ob mouse.
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Figure 3: Sulfatide in pancreas of ob/ob mice. Thin layer chromatography of sulfatide in whole pancreas isolated from ob/ob mice given C16:0 sulfatide. The extraction and lipid isolation procedure as well as the TLC-ELISA method are described under "Methods". Lipids corresponding to approximately 3–4 mg tissue protein were applied (one representative plate is shown). Lane 1, seminolipid (120 pmol); lane 2, sulfated lactosylceramide (20 pmol); lane 3, sulfatide (40 pmol); lane 4, C16:0 sulfatide standard (25 pmol); lane 5, 6 hours after administration; lane 6, 24 hours after administration and lane 7, sulfatide isolated from untreated ob/ob mouse.

Mentions: The total sulfatide amount (endogenous pool plus exogenous uptake) in the pancreatic tissue of ob/ob mice was examined using TLC-ELISA. Mice receiving C16:0 sulfatide showed significantly lower pancreatic sulfatide concentrations 24 hours after administration (54 ± 9 pmol/mg protein, n = 6, p < 0.05) than 6 hours after sulfatide administration (85 ± 10 pmol/mg protein, n = 6). Thus, these results lent support to the idea of sulfatide being degraded 24 hours after administration. Further support for an uptake of C16:0 sulfatide in pancreas 6 hours after i.p administration is the occurrence of a slow migrating band on the TLC plate (Figure 3, lane 5, lower band), as compared with sulfatide isolated from pancreas of ob/ob mice previously reported [3] (Fig. 3, lane 7), where mainly long-chain sulfatide isoforms were found (upper band).


Uptake of the glycosphingolipid sulfatide in the gastrointestinal tract and pancreas in vivo and in isolated islets of Langerhans.

Blomqvist M, Osterbye T, Månsson JE, Buschard K, Fredman P - Lipids Health Dis (2006)

Sulfatide in pancreas of ob/ob mice. Thin layer chromatography of sulfatide in whole pancreas isolated from ob/ob mice given C16:0 sulfatide. The extraction and lipid isolation procedure as well as the TLC-ELISA method are described under "Methods". Lipids corresponding to approximately 3–4 mg tissue protein were applied (one representative plate is shown). Lane 1, seminolipid (120 pmol); lane 2, sulfated lactosylceramide (20 pmol); lane 3, sulfatide (40 pmol); lane 4, C16:0 sulfatide standard (25 pmol); lane 5, 6 hours after administration; lane 6, 24 hours after administration and lane 7, sulfatide isolated from untreated ob/ob mouse.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC1622747&req=5

Figure 3: Sulfatide in pancreas of ob/ob mice. Thin layer chromatography of sulfatide in whole pancreas isolated from ob/ob mice given C16:0 sulfatide. The extraction and lipid isolation procedure as well as the TLC-ELISA method are described under "Methods". Lipids corresponding to approximately 3–4 mg tissue protein were applied (one representative plate is shown). Lane 1, seminolipid (120 pmol); lane 2, sulfated lactosylceramide (20 pmol); lane 3, sulfatide (40 pmol); lane 4, C16:0 sulfatide standard (25 pmol); lane 5, 6 hours after administration; lane 6, 24 hours after administration and lane 7, sulfatide isolated from untreated ob/ob mouse.
Mentions: The total sulfatide amount (endogenous pool plus exogenous uptake) in the pancreatic tissue of ob/ob mice was examined using TLC-ELISA. Mice receiving C16:0 sulfatide showed significantly lower pancreatic sulfatide concentrations 24 hours after administration (54 ± 9 pmol/mg protein, n = 6, p < 0.05) than 6 hours after sulfatide administration (85 ± 10 pmol/mg protein, n = 6). Thus, these results lent support to the idea of sulfatide being degraded 24 hours after administration. Further support for an uptake of C16:0 sulfatide in pancreas 6 hours after i.p administration is the occurrence of a slow migrating band on the TLC plate (Figure 3, lane 5, lower band), as compared with sulfatide isolated from pancreas of ob/ob mice previously reported [3] (Fig. 3, lane 7), where mainly long-chain sulfatide isoforms were found (upper band).

Bottom Line: The glycosphingolipid sulfatide has previously been found in several mammalian tissues, but information on the uptake of exogenously administered sulfatide in different organs in vivo is limited.Exogenously administered sulfatide was found in pancreas after i.p, but not after oral administration.Our study supports a selective uptake and/or preservation of sulfatide in the gastrointestinal tract after oral administration and with emphasises on pancreatic sulfatide uptake, i.p. administration results in sulfatide at relevant location.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Neuroscience and Physiology, Psychiatry and Neurochemistry Section, The Sahlgrenska Academy at Göteborg University, Sahlgrenska University Hospital/Mölndal, SE-431 80 Mölndal, Sweden. maria.blomqvist@gu.se

ABSTRACT

Background: The glycosphingolipid sulfatide has previously been found in several mammalian tissues, but information on the uptake of exogenously administered sulfatide in different organs in vivo is limited. In pancreatic beta cells, sulfatide has been shown to be involved in insulin processing and secretion in vitro. In this study, we examined the uptake of exogenously administered sulfatide and its distribution to the pancreatic beta cells. This might encourage future studies of the function(s) of sulfatide in beta cell physiology in vivo. Radioactive sulfatide was given orally to mice whereafter the uptake of sulfatide in the gastrointestinal tract and subsequent delivery to the pancreas was examined. Sulfatide uptake in pancreas was also studied in vivo by i.p. administration of radioactive sulfatide in mice, and in vitro in isolated rat islets. Isolated tissue/islets were analysed by scintillation counting, autoradiography and thin-layer chromatography-ELISA.

Results: Sulfatide was taken up in the gastrointestinal tract for degradation or further transport to other organs. A selective uptake of short chain and/or hydroxylated sulfatide fatty acid isoforms was observed in the small intestine. Exogenously administered sulfatide was found in pancreas after i.p, but not after oral administration. The in vitro studies in isolated rat islets support that sulfatide, independently of its fatty acid length, is endocytosed and metabolised by pancreatic islets.

Conclusion: Our study supports a selective uptake and/or preservation of sulfatide in the gastrointestinal tract after oral administration and with emphasises on pancreatic sulfatide uptake, i.p. administration results in sulfatide at relevant location.

Show MeSH