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Kinetics of progenitor hemopoetic stem cells in sepsis: correlation with patients survival?

Tsaganos T, Giamarellos-Bourboulis EJ, Kollias S, Zervakis D, Karagianni V, Pelekanou A, Tampaki EC, Kontogiorgi M, Koroneos A, Drakoulis N, Armaganidis A, Roussos C, Giamarellou H - BMC Infect. Dis. (2006)

Bottom Line: Positive correlations were found between the absolute CD34/CD45 count and the absolute monocyte count on days 1, 5 and 7.Stem cells are increased in peripheral blood over all days of follow-up compared to healthy volunteers.Patients with counts on day 1 less than 310/microl are accompanied by increased survival compared to patients with more than 310/microl.

View Article: PubMed Central - HTML - PubMed

Affiliation: 4th Department of Internal Medicine, University of Athens, Medical School, Greece. tsag@freemail.gr

ABSTRACT

Background: Current theories underline the crucial role of pro-inflammatory mediators produced by monocytes for the pathogenesis of sepsis. Since monocytes derive from progenitor hemopoetic cells, the kinetics of stem cells was studied in peripheral blood of patients with sepsis.

Methods: Blood was sampled from 44 patients with septic syndrome due to ventilator-associated pneumonia on days 1, 3, 5 and 7 upon initiation of symptoms. Concentrations of tumour necrosis factor-alpha (TNFalpha), interleukin (IL)-6, IL-8 and G-CSF were estimated by ELISA. CD34/CD45 cells were determined after incubation with anti-CD45 FITC and anti-CD34 PE monocloncal antibodies and flow cytometric analysis. Samples from eight healthy volunteers served as controls.

Results: Median of CD34/CD45 absolute count of controls was 1.0/mul. Respective values of the total study population were 123.4, 112.4, 121.5 and 120.9/mul on days 1, 3, 5 and 7 (p < 0.0001 compared to controls). Positive correlations were found between the absolute CD34/CD45 count and the absolute monocyte count on days 1, 5 and 7. Survival was prolonged among patients with less than 310/microl CD34/CD45 cells on day 1 compared to those with more than 310/microl of CD34/CD45 cells (p: 0.022). Hazard ratio for death due to sepsis was 5.47 (p: 0.039) for CD34/CD45 cells more than 310/microl. Median IL-6 on day 1 was 56.78 and 233.85 pg/ml respectively for patients with less than 310/microl and more than 310/microl CD34/CD45 cells (p: 0.021).

Conclusion: Stem cells are increased in peripheral blood over all days of follow-up compared to healthy volunteers. Patients with counts on day 1 less than 310/microl are accompanied by increased survival compared to patients with more than 310/microl.

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Absolute blood counts of CD34/CD45 cells over daily follow-up of patients with ventilator-associated pneumonia (VAP) and sepsis, severe sepsis or septic shock. Circles denote outliers and asterisks denote extremes. ap < 0.05 compared to sepsis.
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Figure 1: Absolute blood counts of CD34/CD45 cells over daily follow-up of patients with ventilator-associated pneumonia (VAP) and sepsis, severe sepsis or septic shock. Circles denote outliers and asterisks denote extremes. ap < 0.05 compared to sepsis.

Mentions: Clinical characteristics of patients enrolled in the study are shown in Table 1. Median ± SE of CD34/CD45 cells of controls was 1.0 ± 3.2/μl. Respective values of the total study population were 123.4 ± 61.6, 112.4 ± 96.8, 121.5 ± 96.8 and 120.9 ± 30.6/μl on days 1, 3, 5 and 7 of follow-up respectively (p of comparisons of all days to controls less than 0.0001). In these days median ± SE of MFI were 6.05 ± 0.33, 6.55 ± 0.33, 6.10 ± 0.58 and 6.15 ± 0.55 respectively. Follow-up values of CD34/CD45 cells separately for patients with sepsis, severe sepsis and septic shock are shown in Figure 1.


Kinetics of progenitor hemopoetic stem cells in sepsis: correlation with patients survival?

Tsaganos T, Giamarellos-Bourboulis EJ, Kollias S, Zervakis D, Karagianni V, Pelekanou A, Tampaki EC, Kontogiorgi M, Koroneos A, Drakoulis N, Armaganidis A, Roussos C, Giamarellou H - BMC Infect. Dis. (2006)

Absolute blood counts of CD34/CD45 cells over daily follow-up of patients with ventilator-associated pneumonia (VAP) and sepsis, severe sepsis or septic shock. Circles denote outliers and asterisks denote extremes. ap < 0.05 compared to sepsis.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC1586204&req=5

Figure 1: Absolute blood counts of CD34/CD45 cells over daily follow-up of patients with ventilator-associated pneumonia (VAP) and sepsis, severe sepsis or septic shock. Circles denote outliers and asterisks denote extremes. ap < 0.05 compared to sepsis.
Mentions: Clinical characteristics of patients enrolled in the study are shown in Table 1. Median ± SE of CD34/CD45 cells of controls was 1.0 ± 3.2/μl. Respective values of the total study population were 123.4 ± 61.6, 112.4 ± 96.8, 121.5 ± 96.8 and 120.9 ± 30.6/μl on days 1, 3, 5 and 7 of follow-up respectively (p of comparisons of all days to controls less than 0.0001). In these days median ± SE of MFI were 6.05 ± 0.33, 6.55 ± 0.33, 6.10 ± 0.58 and 6.15 ± 0.55 respectively. Follow-up values of CD34/CD45 cells separately for patients with sepsis, severe sepsis and septic shock are shown in Figure 1.

Bottom Line: Positive correlations were found between the absolute CD34/CD45 count and the absolute monocyte count on days 1, 5 and 7.Stem cells are increased in peripheral blood over all days of follow-up compared to healthy volunteers.Patients with counts on day 1 less than 310/microl are accompanied by increased survival compared to patients with more than 310/microl.

View Article: PubMed Central - HTML - PubMed

Affiliation: 4th Department of Internal Medicine, University of Athens, Medical School, Greece. tsag@freemail.gr

ABSTRACT

Background: Current theories underline the crucial role of pro-inflammatory mediators produced by monocytes for the pathogenesis of sepsis. Since monocytes derive from progenitor hemopoetic cells, the kinetics of stem cells was studied in peripheral blood of patients with sepsis.

Methods: Blood was sampled from 44 patients with septic syndrome due to ventilator-associated pneumonia on days 1, 3, 5 and 7 upon initiation of symptoms. Concentrations of tumour necrosis factor-alpha (TNFalpha), interleukin (IL)-6, IL-8 and G-CSF were estimated by ELISA. CD34/CD45 cells were determined after incubation with anti-CD45 FITC and anti-CD34 PE monocloncal antibodies and flow cytometric analysis. Samples from eight healthy volunteers served as controls.

Results: Median of CD34/CD45 absolute count of controls was 1.0/mul. Respective values of the total study population were 123.4, 112.4, 121.5 and 120.9/mul on days 1, 3, 5 and 7 (p < 0.0001 compared to controls). Positive correlations were found between the absolute CD34/CD45 count and the absolute monocyte count on days 1, 5 and 7. Survival was prolonged among patients with less than 310/microl CD34/CD45 cells on day 1 compared to those with more than 310/microl of CD34/CD45 cells (p: 0.022). Hazard ratio for death due to sepsis was 5.47 (p: 0.039) for CD34/CD45 cells more than 310/microl. Median IL-6 on day 1 was 56.78 and 233.85 pg/ml respectively for patients with less than 310/microl and more than 310/microl CD34/CD45 cells (p: 0.021).

Conclusion: Stem cells are increased in peripheral blood over all days of follow-up compared to healthy volunteers. Patients with counts on day 1 less than 310/microl are accompanied by increased survival compared to patients with more than 310/microl.

Show MeSH
Related in: MedlinePlus