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An Application of Molecular Genotyping in Mice.

Underkoffler LA, Collins JN, Choi JD, Oakey RJ - Biol Proced Online (2003)

Bottom Line: Microsatellite markers are simple sequence repeats within the mammalian genome that can be used for identifying disease loci, mapping genes of interest as well as studying segregation patterns related to meiotic nondisjunction.Molecular genotyping offers such identification at any developmental stage, which allows for a broad range of anomalies to be studied.Information on the parental origin as well as the number of chromosomes a given progeny carried was obtained in our analysis.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Human Genetics, The Children's Hospital of Philadelphia. Philadelphia, PA 19104. USA.

ABSTRACT
Microsatellite markers are simple sequence repeats within the mammalian genome that can be used for identifying disease loci, mapping genes of interest as well as studying segregation patterns related to meiotic nondisjunction. Different strains of mice have variable CA repeat lengths and PCR based methods can be used to identify them, thus allowing for specific genotypes to be assigned. Molecular genotyping offers such identification at any developmental stage, which allows for a broad range of anomalies to be studied. We studied chromosomal segregation in relation to nondisjunction in early-gestation mouse embryos using molecular genotyping. Information on the parental origin as well as the number of chromosomes a given progeny carried was obtained in our analysis.

No MeSH data available.


Related in: MedlinePlus

A denaturing polyacrylamide genotyping gel separating radioactive PCR products from various mouse strains. The polymorphic parental strains of mice are shown in lanes marked B (C57BL/6JEi-Rb(7.18)9Lub), R (Rb(2.8)2Lub / (7.18)9Lub ), C (M.m. Castaneus), D (DBA/2J) and 3 (C3H/HeJ). Genotypes in bold type-face indicate aneuploid genotypes. BR3 is a trisomy (it shows three chromosome 7 bands); DR & B3 are both uniparental disomies (UpDi) since the mother of DR was a DXR animal and the mother of B3 was a BX3 animal. No paternal chromosome 7 was present in the genotypes of these two animals. All the other genotypes indicate inheritance of chromosome 7 from both parents and confirm a normal chromosomal inheritance for these embryos.
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Figure 3: A denaturing polyacrylamide genotyping gel separating radioactive PCR products from various mouse strains. The polymorphic parental strains of mice are shown in lanes marked B (C57BL/6JEi-Rb(7.18)9Lub), R (Rb(2.8)2Lub / (7.18)9Lub ), C (M.m. Castaneus), D (DBA/2J) and 3 (C3H/HeJ). Genotypes in bold type-face indicate aneuploid genotypes. BR3 is a trisomy (it shows three chromosome 7 bands); DR & B3 are both uniparental disomies (UpDi) since the mother of DR was a DXR animal and the mother of B3 was a BX3 animal. No paternal chromosome 7 was present in the genotypes of these two animals. All the other genotypes indicate inheritance of chromosome 7 from both parents and confirm a normal chromosomal inheritance for these embryos.

Mentions: We intercrossed mice heterozygous for a (7.18) Robertsonian translocation (14) and have genotyped 1,812 embryos from 364 litters with known parental origin, strain and age (15). The key feature of the strains of mice used in these types of studies is that they are inbred strains with defined genetic markers. As a result, DNA polymorphisms between strains of mice are readily identified and the number and parent of origin of each chromosome are readily determined. Thus aneuploid progeny from crosses involving trisomy or monosomy as well as uniparental inheritance for a particular chromosome can be easily identified (Figures 2 and 3) and the frequency of nondisjunction determined.


An Application of Molecular Genotyping in Mice.

Underkoffler LA, Collins JN, Choi JD, Oakey RJ - Biol Proced Online (2003)

A denaturing polyacrylamide genotyping gel separating radioactive PCR products from various mouse strains. The polymorphic parental strains of mice are shown in lanes marked B (C57BL/6JEi-Rb(7.18)9Lub), R (Rb(2.8)2Lub / (7.18)9Lub ), C (M.m. Castaneus), D (DBA/2J) and 3 (C3H/HeJ). Genotypes in bold type-face indicate aneuploid genotypes. BR3 is a trisomy (it shows three chromosome 7 bands); DR & B3 are both uniparental disomies (UpDi) since the mother of DR was a DXR animal and the mother of B3 was a BX3 animal. No paternal chromosome 7 was present in the genotypes of these two animals. All the other genotypes indicate inheritance of chromosome 7 from both parents and confirm a normal chromosomal inheritance for these embryos.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC154566&req=5

Figure 3: A denaturing polyacrylamide genotyping gel separating radioactive PCR products from various mouse strains. The polymorphic parental strains of mice are shown in lanes marked B (C57BL/6JEi-Rb(7.18)9Lub), R (Rb(2.8)2Lub / (7.18)9Lub ), C (M.m. Castaneus), D (DBA/2J) and 3 (C3H/HeJ). Genotypes in bold type-face indicate aneuploid genotypes. BR3 is a trisomy (it shows three chromosome 7 bands); DR & B3 are both uniparental disomies (UpDi) since the mother of DR was a DXR animal and the mother of B3 was a BX3 animal. No paternal chromosome 7 was present in the genotypes of these two animals. All the other genotypes indicate inheritance of chromosome 7 from both parents and confirm a normal chromosomal inheritance for these embryos.
Mentions: We intercrossed mice heterozygous for a (7.18) Robertsonian translocation (14) and have genotyped 1,812 embryos from 364 litters with known parental origin, strain and age (15). The key feature of the strains of mice used in these types of studies is that they are inbred strains with defined genetic markers. As a result, DNA polymorphisms between strains of mice are readily identified and the number and parent of origin of each chromosome are readily determined. Thus aneuploid progeny from crosses involving trisomy or monosomy as well as uniparental inheritance for a particular chromosome can be easily identified (Figures 2 and 3) and the frequency of nondisjunction determined.

Bottom Line: Microsatellite markers are simple sequence repeats within the mammalian genome that can be used for identifying disease loci, mapping genes of interest as well as studying segregation patterns related to meiotic nondisjunction.Molecular genotyping offers such identification at any developmental stage, which allows for a broad range of anomalies to be studied.Information on the parental origin as well as the number of chromosomes a given progeny carried was obtained in our analysis.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Human Genetics, The Children's Hospital of Philadelphia. Philadelphia, PA 19104. USA.

ABSTRACT
Microsatellite markers are simple sequence repeats within the mammalian genome that can be used for identifying disease loci, mapping genes of interest as well as studying segregation patterns related to meiotic nondisjunction. Different strains of mice have variable CA repeat lengths and PCR based methods can be used to identify them, thus allowing for specific genotypes to be assigned. Molecular genotyping offers such identification at any developmental stage, which allows for a broad range of anomalies to be studied. We studied chromosomal segregation in relation to nondisjunction in early-gestation mouse embryos using molecular genotyping. Information on the parental origin as well as the number of chromosomes a given progeny carried was obtained in our analysis.

No MeSH data available.


Related in: MedlinePlus